Age-related changes in S100beta protein immunoreactivity in the periaqueductal gray (PAG) in rats

• Autorzy: Rycerz K. , Krawczyk A. , Jaworska-Adamu J. , Krawczyk-Marc I. , Szalak R.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate and compare S100β protein immunoreactivity in astrocytes of the periaqueductal gray in young and aged rats and to evaluate the morphology of these cells. Furthermore, the amount, astrocyte surface areas, and digital immunostaining intensity of the protein were morphometrically analysed. The research was conducted on 100-day-old and 3-year-old male rats. Midbrain sections, containing PAG, were obtained from the animals. To detect S100β protein in astrocytes, the peroxydase-antiperoxydase immunohistochemical reaction with the S100β antibody was performed. Diaminobenzidine was used as a chromogen. For this method, specificity control was carried out. S100β-immunopositive astrocytes from the dorsal, dorsolateral, and ventrolateral parts of PAG were observed and photographed with a light microscope equipped with a digital camera. Morphometric analyses were performed. In 3-year-old rats, astrocytes in all parts of PAG were characterised by similar S100β immunoreaction intensity as those in 100-day-old animals. Astrocyte nuclei were round or oval-shaped, and showed very weak, moderate or intensive immunostaining. Astrocytes in all parts of PAG were irregularly spread. Morphometric analyses confirmed the results of microscopic examination. There were no statistically significant differences in the number of glial cells between the three parts of PAG. The surface areas of astrocytes in all parts did not differ significantly. Digital immunostaining intensity analysis revealed slight differences in the dorsal and ventrolateral parts of PAG between the two age groups of rats. Our results and the available literature data indicate that S100β protein expression in young and aged animals may differ in various brain areas and depends on many factors.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 03
• Opis fizyczny: p.158-162,fig.,ref.

Twórcy

autor

Rycerz K.

• Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland

autor

Krawczyk A.

• Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland

autor

Jaworska-Adamu J.

• Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland

autor

Krawczyk-Marc I.

• Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland

autor

Szalak R.

• Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland

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