PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 20 | 2 |

Tytuł artykułu

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

Wydawca

-

Rocznik

Tom

20

Numer

2

Opis fizyczny

p.273-275,fig.,ref.

Twórcy

autor
  • Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
autor
  • Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
autor
  • Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Slovak Academy of Sciences, Kosice, Slovakia
autor
  • Institute of Parasitology and Institute of Zoology, Slovak Academy of Sciences, Kosice, Slovakia

Bibliografia

  • 1. Literak I, Vanko R, Dolejska M, Cizek A, Karpiskova R. Antibiotic resistant Escherichia coli and Salmonella in Russian rooks (Corvusfrugilegus) wintering in the Czech Republic. Letters Appl Microbiol.2007; 45(6): 616–621.
  • 2. Mosansky L, Trnka A. The rook (Corvus frugilegus), pp. 581–584. In: Danko S, Darolova A, Kristin A. (eds.), Birds distribution in Slovakia.Veda, Bratislava 2002, 688 pp.
  • 3. Danko S. The results of own ringed birds in eastern Slovakia in 1966– 1999. Tichodroma 2000; 13: 205–226.
  • 4. Kuban V, Matousek B. Results of bird ringing by William Kuban in Slovakia in the years 1960–1999. Tichodroma 1999; 12: 136–215.
  • 5. Literak I, Micudova M, Tausova D, Cizek A, Dolejska M, Papousek I, Prochazka J, Vojtech J, Borleis F, Guardone L, Guenther S, Hordowski J,Lejas C, Meissner W, Marcos BF, Tucakov M. Microbial Drug Resistence2012; DOI:10.1089/mdr.2012.0075.
  • 6. Gregova G, Kmetova M, Kmet V, Venglovsky J, Feher A. Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse.Ann Agric Environ Med. 2012; 19(1):75–77.
  • 7. CLSI (Clinical Laboratory Standards Institute): Performance standards for antimicrobial disk and dilution. Susceptibility tests for bacteriaisolated from animals; approved standard (3rd edn). CLSI DocumentM13-A3 28, 2008; 1–99.
  • 8. Gattringer R, Niks M, Ostertag R, Schwarz K, Medvedovic H, Graninger W, Georgopoulos A. Evaluation of Miditech automated colorimetricMIC reading for antimicrobial susceptibility testing. J AntimicrobChemother. 2002; 49(4): 651–659.
  • 9. Carattoli A, Garcia-Fernandez A, Varesi P, Fortini D, Gerardi S, Penni A, Mancini C, Giordano A. Molecular Epidemiology of Escherichia coliProducing Extended-Spectrum ß-Lactamases Isolated in Rome, Italy.J Clin Microbiol. 2008; 46(1): 103–108.
  • 10. Pérez-Pérez PJ, Hanson ND. Detection of Plasmid-Mediated AmpC beta-Lactamase Genes in Clinical Isolates by Using Multiplex PCR.J Clin Microbiol. 2002; 40(6) 2153– 2162.
  • 11. Robicsek A, Strahilevitz J, Sahm DF, Jacoby GA, Hooper DC. Qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from theUnited States. Antimicrob Agents Chemother. 2006; 50(8): 2872–2874.
  • 12. Mazel D, Dychinco B, Webb V. A, Davies J. Antibiotic resistance in the ECOR collection: Integrons and identification of a novel aad gene.Antimicrob Agents Chemother. 2000; 44(6): 1568–1574.
  • 13. Johnson JR, Stell AL. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and hostcompromise. J Infect Dis. 2000; 181(1): 261–272.
  • 14. Foley SL, Home SM, Giddings CW, Robinson M, Nolan LK. Iss from a virulent avian Escherichia coli. Avian Diseases 2000; 44(1): 185–191.
  • 15. Dozois CM, Dho-Moulin M, Brée A, Fairbrother JM, Desautels C, Curtiss III. R. Relation between the Tsh autotransporter andpathogenicity of avian Escherichia coli and localization and analysisof the tsh genetic region. Infect Immun. 2000; 68(7): 4145–4154.
  • 16. Germon P, Chen YH., He L, Blanco J E, Brée A, Schouler SH. IbeA, a virulence factor of avian pathogenic Escherichia coli. Microbiology 2005; 151(4): 1179–1186.
  • 17. Le Bouguénec C, Archambaud M, Labigne A. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J Clin Microbiol. 1992; 30(5): 1189–1193.
  • 18. Carattoli A. Animal reservoirs for extended spectrum beta lactamase producers. Clin Microbiol Infect. 2008; 14(Suppl.1): 117–123.
  • 19. EFSA report. The European Union Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2010. EFSA Journal 2012; 10(3): 1–233.
  • 20. Drugdova Z, Kmet V, Bujnakova D. Virulence factors in Escherichia coli isolated from chicken meat in Slovakia. J Food Nutr Res. 2010;49(1): 10–13.
  • 21. Toma C, Higa N, Iyoda S, Rivas M, Iwanaga M. The long polar fimbriae genes identified in Shiga toxin-producing Escherichia coli are presentin other diarrheagenic E. coli and in the standard E. coli collection ofreference (ECOR) strains. Res Microbiol. 2006; 157(2): 153–161.
  • 22. Folk C, Beklova M. Die Winternahrung der Saatkrähe – Corvus frugilegus L. – im städtischen Milieu. Folia Zool. 1971; 20(4): 357-363.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-f79c6018-0e08-4e5c-9be1-04b3b1586a2b
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.