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2017 | 20 | 3 |
Tytuł artykułu

Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4/91 by TaqMan real-time PCR

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 x 10³ copies of the Ma5 strain, and 3.852 x 10³ copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
20
Numer
3
Opis fizyczny
p.599-601,ref.
Twórcy
autor
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
autor
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
autor
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
autor
  • Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
Bibliografia
  • Boursnell ME, Brown TD, Foulds IJ, Green PF, Tomley FM, Binns MM (1987) Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol 68: 57-77.
  • Cavanagh D, Gelb J (2008) Infectious bronchitis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE (eds). Diseases of Poultry 12th ed., Blackwell Publishing Professional, Ames, Iowa, USA, pp 117-135.
  • Cavanagh D, Davis PJ, Cook JK, Li D, Kant A, Koch G (1992) Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathol 21: 33-43.
  • Cook JK, Orbell SJ, Woods MA, Huggins MB (1999) Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes. Avian Pathol 28: 477-485.
  • Koch G, Hartog L, Kant A, van Roozelaar DJ (1990) Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. J Gen Virol 71: 1929-1935.
  • Ladman BS, Loupos AB, Gelb J Jr. (2006) Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization. Avian Pathol 35: 127-133.
  • Meir R, Maharat O, Farnushi Y, Simanov L (2010) Development of a real-time TaqMan RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation. J Virol Methods 163: 190-194.
  • Sjaak de Wit JJ, Cook JK, van der Heijden HM (2011) Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol 40: 223-235.
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739.
  • Wickramasinghe IN, van Beurden SJ, Weerts EA, Verheije MH (2014) The avian coronavirus spike protein. Virus Res 194: 37-48.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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