The heavy-metal resistance determinant of newly isolated bacterium from a nickel-contaminated soil in southwest Slovakia

• Autorzy: Remenar M. , Kamlarova A. , Harichova J. , Zamocky M. , Ferianc P.
• Języki publikacji: EN

Abstrakty

EN

A bacterial isolate MR-CH-I2 [KC809939] isolated from soil contaminated mainly by high nickel concentrations in southwest Slovakia was previously found carrying nccA-like heavy-metal resistance determinant, marked as MR-CH-I2-HMR [KF218096]. According to phylogenetic analysis of short (696 bp) 16S rDNA (16S rRNA) sequences this bacterium was tentatively assigned to Uncultured beta proteobacterium clone GC0AA7ZA05PP1 [JQ913301]. nccA-like gene product was on the same base of its partial (581 bp) sequences tentatively assigned to CzcA family heavy metal efflux pump [YP_001899332] from Ralstonia picketii 12J with 99% similarity. In this study the bacterium MR-CH-I2 and its heavy-metal resistance determinant were more precisely identified. This bacterial isolate was on the base of phylogenetic analysis of almost the whole (1,500 bp) 16S rDNA (16S rRNA) sequence, MR-CH-I2 [MF102046], and sequence for gyrB gene and its product respectively, MR-CH-I2-gyrB [MF134666], assigned to R. picketii 12J [CP001068] with 99 and 100% similarities, respectively. In addition, the whole nccA-like heavy-metal resistance gene sequence (3,192 bp), marked as MR-CH-I2-nccA [KR476581], was obtained and on the base of phylogenetic analysis its assignment was confirmed to MULTISPECIES: cation efflux system protein CzcA [WP_004635342] from Burkholderiaceae with 98% similarity. Furthermore, although the bacterium carried one high molecular plasmid of about 50 kb in size, nccA-like gene was not located on this plasmid. Finally, the results from RT-PCR analysis showed that MR-CH-I2-nccA gene was significantly induced only by the addition of nickel.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 2
• Opis fizyczny: p.191-201,fig.,ref.

Twórcy

autor

Remenar M.

• Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia

autor

Kamlarova A.

• Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia

autor

Harichova J.

• Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia

autor

Zamocky M.

• Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia
• Metalloprotein Research Group, Division of Biochemistry, Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria

autor

Ferianc P.

• Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia

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Identyfikatory

DOI

10.21307/pjm-2018-022