The estimation of antifungal activity of essential oil and hydrosol obtained from wrinkled-leaf mint (Mentha crispa L.)

• Autorzy: Wojcik-Stopczynska B. , Jakowienko P. , Wysocka G.

Warianty tytułu

PL

Ocena aktywności antygrzybiczej olejku eterycznego i hydrozolu uzyskanych z mięty kędzierzawej (Mentha crispa L.)

• Języki publikacji: EN

Abstrakty

EN

The effects of essential oil and hydrosol extracted from dried aerial parts of wrinkled-leaf mint (Mentha crispa L.), were determined against Aspergillus fumigatus, Aspergillus parasiticus, Botrytis cinerea, Cladosporium herbarum, Fusarium oxysporum and Penicillium cyclopium growth. The influence of oil was estimated by disc diffusion agar method and expressed (in mm) as inhibition growth zones of tested strains. Furthermore, the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of oil were determined by microdilution method in liquid medium. Chromatography analysis showed that the main components of oil were: piperitenone (29.9%), pulegone (13.9%) and 1,8-cineole (10.5%). The oil proved the highest inhibitory action against B. cinerea (MFC≤0.32 μl·ml-1, inhibition zone 50.0 mm) and C. herbarum (MFC=0.63 μl·ml-1, inhibition zone 59.3mm), and the least – against A. parasiticus and F. oxysporum, (inhibition zones respectively 7.0 and 12.7 mm, MIC=5.0 μl·ml-1, and MFC=10.0 μl·ml-1). Activity of hydrosol was expressed as a percent of inhibition growth of fungi which was calculated on the ground of diameters of colonies growing on agar medium with addition of 10 and 15% hydrosol and diameters of colonies on control medium. Data showed that antifungal activity of hydrosol was weak. It in higher amounts inhibited the growth of A. fumigatus (by 8.7 and 15.9%, respectively, at a concentration in medium of 10 and 15%) and stimulated growth of B. cinerea and P. cyclopium.

PL

Określono działanie olejku eterycznego i hydrozolu, otrzymanych z wysuszonych, nadziemnych pędów mięty kędzierzawej (Mentha crispa L.), na wzrost grzybów: Aspergillus fumigatus, Aspergillus parasiticus, Botrytis cinerea, Cladosporium herbarum, Fusarium oxysporum i Penicillium cyclopium. Wpływ olejku zbadano metodą krążkową, mierząc (w mm) strefy zahamowania wzrostu grzybów. Ponadto wyznaczono dla olejku minimalne stężenie hamujące (MIC) oraz grzybobójcze (MFC), stosując metodę mikrorozcieńczeń w podłożu płynnym. Analiza chromatograficzna wykazała, że głównymi składnikami olejku były: piperytenon (29,9%), pulegon (13,9%) i 1,8-cyneol (10,5%). Olejek wykazywał najwyższą aktywność wobec B. cinerea (MFC ≤0,32 μl·ml-1, strefa zahamowania 50,0 mm) oraz C. herbarum (MFC=0,63 μl·ml-1, strefa zahamowania 59,3 mm), natomiast najmniej ograniczał wzrost A. parasiticus i F. oxysporum (strefy zahamowania odpowiednio 7,0 i 12,7 mm, MIC=5,0 μl·ml-1, a MFC=10,0 μl·ml-1). Aktywność hydrozolu wyrażano jako procent zahamowania wzrostu grzybów, który obliczano na podstawie wielkości średnic kolonii rosnących na podłożu agarowym z dodatkiem 10 i 15% hydrozolu oraz średnic kolonii na podłożu kontrolnym. Badania wykazały, że działanie przeciwgrzybicze hydrozolu było słabe. Najbardziej hamował on wzrost A. fumigatus (o 8,7 i 15,9%, odpowiednio przy stężeniu w podłożu 10 i 15%), a stymulował wzrost B. cinerea i P. cyclopium.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2012
• Tom: 58
• Numer: 1
• Opis fizyczny: p.5-15,ref.

Twórcy

autor

Wojcik-Stopczynska B.

• Department of Horticulture, Laboratory of Storage and Processing, West Pomeranian University of Technology in Szczecin, Slowackiego 17, 71-434 Szczecin, Poland

autor

Jakowienko P.

autor

Wysocka G.

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