Physiological and molecular response of annual Medicago species to juglone

• Autorzy: Torabi Z. , Rafiei F. , Shabani L. , Danesh Shahraki A.
• Języki publikacji: EN

Abstrakty

EN

The current study was conducted to evaluate the physiological and molecular response of self-regenerating annual Medicago species (M. polymorpha and M. lupulina) to juglone exposure. A randomized complete block design was performed in which two treatment groups consisted of a control and juglone (10-4 M) allotted to main plots and genotypes assigned to subplots. A significant increase in the concentration of GSH and the GSH/ GSSG ratio was observed in both annual Medicago species in response to juglone exposure. However, such response was greater in M. lupulina than M. polymorpha. The activity of all antioxidant enzymes (CAT, APX, GST, and GPOX) was significantly increased by juglone. In response to juglone, the expression of WRKY was significantly decreased. The transcription of CBF4, Zpt2-2, CAT, and GST genes was highly induced by juglone in annual Medicago species. Higher expression of CBF4, Zpt2-2, CAT and GST genes in M. lupulina which showed more juglone-tolerance can be associated with higher tolerance against allelochemical stress. It can be concluded that M. polymorpha is juglone-sensitive because it started to show chlorosis of leaves after a week subjecting to juglone. Though M. lupulina seems to be more juglone-tolerant at least in short term, long-term exposure of juglone should also be examined to find out its suitability to grow in walnut orchards. Considering the presence of cross-talk between different stresses, we can propose that M. lupulina is more tolerant to other abiotic stresses compared to M. polymorpha.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 248 [9 p.], fig.,ref.

Twórcy

autor

Torabi Z.

• Department of Plant Breeding and Biotechnology, College of Agriculture, Shahrekord University, Shahrekord, Iran

autor

Rafiei F.

• Department of Plant Breeding and Biotechnology, College of Agriculture, Shahrekord University, Shahrekord, Iran

autor

Shabani L.

• Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Iran

autor

Danesh Shahraki A.

• Department of Agronomy, College of Agriculture, Shahrekord University, Shahrekord, Iran

Bibliografia

• Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol. III, 3rd edn. Verlag Chemie, Deerfield Beach, pp 273-286
• Agarwal PK, Jha B (2010) Transcription factors in plants and ABA dependent and independent abiotic stress signaling. Biol Plantarum 54:201–212
• Bais HP, Vepachedu R, Gilroy S, Callaway RM, Vivanco JM (2003) Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science 301:1377–1380
• Becher M, Talke IN, Krall L, Kramer U (2004) Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri. Plant J 37:251–268
• Behdad A, Maghamni A (2013) On the coexistence mechanisms of a perennial grass with an allelopathic shrub in a semiarid rangeland. Int J Agri Crop Sci 5:2001–2008
• Carmagnol F, Sinet PM, Rapin J, Jerome H (1981) Glutathione-Stransferase of human red blood cells; assay, values in normal subjects and in two pathological circumstances: hyper bilirubinemia and impaired renal function. Clin Chim Acta 117:209–217
• Chi WC, Fu SF, Huang TL, Chen YA, Chen CC, Huang HJ (2011) Identification of transcriptome profiles and signaling pathways for the allelochemical juglone in rice roots. Plant Mol Biol 77:591–607
• Cruz-Ortega R, Ayala-Cordero G, Luisa A (2002) Allelochemical stress produced by the aqueous leachate of Callicarpa acuminata: effects on roots of bean, maize, and tomato. Physiol Plantarum 116:20–27
• de Lorenzo L, Merchan F, Blanchet S, Megı´as M, Frugier F, Crespi M, Sousa C (2007) Differential expression of the TFIIIA regulatory pathway in response to salt stress between Medicago truncatula genotypes. Plant Physiol 145:1521–1532
• Ding J, Sun Y, Xiao CL, Shi K, Zhou YH, Yu JQ (2007) Physiological basis of different allelopathic reactions of cucumber and figleaf gourd plants to cinnamic acid. J Exp Bot 58:3765–3773
• Dixon DP, Cummins L, Cole DJ, Edwards R (1998) Glutathionemediated detoxification systems in plants. Curr Opin Plant Biol 1:258–266
• Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trend Plant Sci 15:573–581
• Esterbauer H, Grill D (1978) Seasonal variation of glutathione and glutathione reductase in needles of Picea abies. Plant Physiol 61:119–121
• Garg N, Manchanda G (2009) ROS generation in plants: boon or bane? Plant Biosys 143:81–96
• Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
• Golisz A, Sugano M, Fujii Y (2008) Microarray expression profiling of Arabidopsis thaliana L. in response to allelochemicals identified in buckwheat. J Exp Bot 59:3099–3109
• Guilfoyle TJ, Hagen G (2007) Auxin response factors. Curr Opin Plant Biol 10:453–460
• Karami M, Rafiei F, Shiran B, Khodambashi M (2015) Comparative response of annual Medicago spp to salinity. Russ J Plant Physiol 62:1–8
• Kim SY, Lim JH, Park MR, Kim YJ, Park TI, Seo YW, Choi KJ, Yun SJ (2005) Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. J Biochem Mol Biol 38:218–224
• Kodaira KS, Qin F, Tran LSP, Maruyama K, Kidokoro S, Fujita Y, Yamaguchi-Shinozaki K (2011) Arabidopsis Cys2/His2 zincfinger proteins AZF1 and AZF2 negatively regulate abscisic acidrepressive and auxin-inducible genes under abiotic stress conditions. Plant Physiol 157:742–756
• Lara-Nunez A, Romero-Romero T, Ventura JL, Blancas V, Anaya AL, Cruz-Ortega R (2006) Allelochemical stress causes inhibition of growth and oxidative damage in Lycopersicon esculentum Mill. Plant, Cell Environ 29:2009–2016
• Li D, Zhang Y, Hu X, Shen X, Ma L, Su Z, Dong J (2011) Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses. BMC Plant Biol 11:109–128
• Lin CC, Kao CH (1999) NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings. Plant Soil 216:147–153
• May MJ, Leaver CJ (1993) Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension culture. Plant Physiol 103:157–166
• Merchan F, de Lorenzo L, Rizzo SG, Niebel A, Manyani H, Frugier F, Sousa C, Crespi M (2007) Identification of regulatory pathways involved in the reacquisition of root growth after salt stress in Medicago truncatula. Plant J 51:1–17
• Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S, Lee H, Stevenson B, Zhu JK (2006) Gain- and loss-of-function mutations in Zat10 enhance the tolerance of plants to abiotic stress. FEBS Lett 580:6537–6542
• Mittova V, Tal M, Volokita M, Guy M (2003) Up-regulation of the leaf mitochondrial and peroxisomal antioxidative systems in response to salt-induced oxidative stress in the wild salt-tolerant tomato species Lycopersicon pennellii. Plant, Cell Environ 26:845–856
• Mullineaux PM, Rausch T (2005) Glutathione, photosynthesis and the redox regulation of stress-responsive gene expression. Photosynth Res 86:459–474
• Nakano Y, Asada K (1981) Purification of ascorbate peroxidase in spinach chloroplasts its inactivation in ascorbate-depleted medium and reactivation by mono-dehydroascorbate radical. Plant Cell Physiol 28:131–140
• Oracz K, Voegele A, Tarkowska D, Jacquemoud D, Turečkova V, Urbanova T, Strnad M, Sliwinska E, Leubner-Metzger G (2012) Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture. Plant Cell Physiol 53:81–95
• Polle A, Rennenberg H (1992) Field studies on Norway spruce trees at high altitudes. II. Defence systems against oxidative stress in needles. New Phytol 121:635–642
• Rafiei F, Torabi Z, Ebrahimie S (2015) Type A response regulators are involved in the plant-microbe interaction. Plant Omic J 8:178–182
• Roxas VP, Lodhi SA, Garrett DK, Mahan JR, Allen RD (2000) Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol 41:1229–1234
• Rushton PJ, Somssich IE, Ringler P, Shen QJ (2010) WRKY transcription factors. Trends Plant Sci 15:247–258
• Rushton DL, Tripathi P, Rabara RC, Lin J, Ringler P, Boken AK, Langum TJ, Smidt L, Boomsma DD, Emme NJ, Chen X, Finer JJ, Shen QJ, Rushton PJ (2012) WRKY transcription factors: Key components in abscisic acid signalling. Plant Biotech J 10:2–11
• Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nature Prot 3:1101–1108
• Sekmen AH, Türkan I, Takio S (2007) Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salt-tolerant Plantago maritima and salt-sensitive Plantago media. Physiol Plantarum 131:399–411
• Singh HP, Batish DR, Arora Kaur S, Kohli K (2006) Alpha-Pinene inhibits growth and induces oxidative stress in roots. Ann Bot 98:1261–1269
• Sytykiewicz H (2011) Expression Patterns of Glutathione Transferase Gene (GstI) in Maize Seedlings Under Juglone-Induced Oxidative Stress. Int J Mol Sci 12:7982–7995
• Taji T, Seki M, Satou M, Sakurai T, Kobayashi M, Ishiyama K, Narusaka Y, Narusaka M, Zhu JK, Shinozaki K (2004) Comparative genomics in salt tolerance between Arabidopsis and Arabidopsis-related halophyte salt cress using Arabidopsis microarray. Plant Physiol 135:1697–1709
• Tausz M, Sircelj H, Grill D (2004) The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid? J Exp Bot 55:1955–1962
• Teriz I (2008) Allelopathic effects of Juglone and decomposed walnut leaf juice on muskmelon and cucumber seed germination and seedling growth. Afric J Biotech 7:1870–1874
• Tivoli B, Baranger A, Sivasithamparam K, Barbetti MJ (2006) Annual medicago: from a model crop challenged by a spectrum of necrotrophic pathogens to a model plant to explore the nature of disease resistance. Annal Bot 98:1117–1128
• Turan S, Tripathy BC (2013) Salt and genotype impact on antioxidative enzymes and lipid peroxidation in two rice cultivars during de-etiolation. Protoplasma 250:209–222
• Voegele A, Graeber K, Oracz K, Tarkowska D, Jacquemoud D, Turečkova V, Urbanova T, Strnad M, Leubner-Metzger G (2012) Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A. J Exp Bot 63:5337–5350
• Weber M, Harada E, Vess C, Roepenack-Lahaye EV, Clemens S (2004) Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri roots identifies nicotianamine synthase, a ZIP transporter and other genes as potentialmetal hyperaccumulation factors. Plant J 37:269–281
• Yu JQ, Ye SF, Zhang MF, Hu WH (2003) Effects of root exudates and aqueous root extracts of cucumber (Cucumis sativus), and allelo-chemicals on photosynthesis and antioxidant enzymes in cucumber. Biochem Syst Ecol 31:129–139

Identyfikatory

DOI

10.1007/s11738-015-1999-0