Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1

• Autorzy: Socha W. , Rola J. , Urban-Chmiel R. , Zmudzinski J.F.
• Języki publikacji: EN

Abstrakty

EN

Bovine herpesvirus-1 (BoHV-1), a causative agent of Infectious Bovine Rhinotracheitis (IBR), is responsible for high economic losses in cattle farming industry. The use of testing methods that allow early detection of BoHV-1-infected animals is a key element of each program of IBR eradication. The aim of the study was to design and evaluate two variants of LAMP isothermal tests with SYBR Green fluorescence probes, specific to the genes encoding gD and gE glycoproteins of BoHV-1. LAMP gE BoHV-1 assay was able to distinguish between gE- and gE+ strains of the virus. Both LAMP gD and gE assays were specific to BoHV-1 and did not react with other related to BoHV-1 alphaherpesviruses. Sensitivity of LAMP gD was 2x10⁴ copies of the viral genome whereas for LAMP gE it was 2x10⁵. Diagnostic sensitivity calculated for LAMP gD was 64.7% whereas for LAMP gE it was 80%. Diagnostic specificity for LAMP gD and LAMP gE was 78.9% and 89.3%, respectively. LAMP assay can be a rapid and simple method of diagnosis of acute BoHV-1 infections and discrimination of gE⁻ strains. However, relatively low diagnostic sensitivity of the method can limit its use in routine diagnostics.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 3
• Opis fizyczny: p.619-622,fig.,ref.

Twórcy

autor

Socha W.

• Department of Virology, National Veterinary Research Institute, Pulawy, Poland

autor

Rola J.

• Department of Virology, National Veterinary Research Institute, Pulawy, Poland

autor

Urban-Chmiel R.

• Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland

autor

Zmudzinski J.F.

• Department of Virology, National Veterinary Research Institute, Pulawy, Poland

Bibliografia

• Belknap EB, Walters LM, Kelling C, Ayers VK, Norris J, McMillen J, Hayhow C, Cochran M, Reddy DN, Wright J, Collins JK (1999) Immunogenicity and protective efficacy of a gE, gG and US2 gene-deleted bovine herpesvirus-1 (BHV-1) vaccine. Vaccine 17: 2297-2305.
• El-Kholy AA, Abdelrahman K, Soliman H (2014) Rapid detection of BoHV-1 genomic DNA by loop – mediated isothermal amplification assay. J Virol Methods 204: 81-85.
• Fuchs M, Hübert P, Detterer J, Rziha HJ (1999) Detection of bovine herpesvirus type 1 in blood from naturally infected cattle by using a sensitive PCR that discriminates between wild-type virus and virus lacking glycoprotein E. J Clin Microbiol 37: 2498-2507.
• GotoM., Honda E., OguraA., NomotoA.,Hanaki KI (2009) Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques 46: 167-172.
• Karthik K, Rathore R, Thomas P, Arun TR, Viswas KN, Dhama K, Agarwal RK (2014) New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination. MethodsX 1: 137-143.
• Pawar SS, Meshram CD, Singh NK, Sonwane AA, Saini M, Rautmare SS, Muglikar DM, Mishra BP, Gupta PK (2014) Rapid detection of bovine herpesvirus 1 in bovine semen by loop-mediated isothermal amplification (LAMP) assay. Arch Virol 159: 641-648.
• Raaperi K, Orro T, Viltrop A (2014) Epidemiology and control of bovine herpesvirus 1 infection in Europe. Vet J 201: 249-256.
• Wernike K, Hoffmann B, Kalthoff D, König P, Beer M (2011) Development and validation of a triplex real-time PCR assay for the rapid detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of Bovine herpesvirus type 1. J Virol Methods 174: 77-84.
• Wiedmann M, Brandon R, Wagner P, Dubovi EJ, Batt CA (1993) Detection of bovine herpesvirus-1 in bovine semen by a nested PCR assay. J Virol Methods, 44: 129-139.
• Zanoli LM, Spoto G (2012) Isothermal amplification methods for the detection of nucleic acids in microfluidic devices. Biosensors (Basel) 3: 18-43.

Identyfikatory

DOI

10.1515/pjvs-2017-0078