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2012 | 21 | 4 |

Tytuł artykułu

Changes in microbial communities of nitrifying immobilized biomass: the role of operational conditions

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Our research determined the impact of operational conditions on the quantity of total and ammonia-oxidizing bacteria (AOB) in immobilized biomass. The experiment was conducted for two kinds of wastewater differing in organic carbon concentration at HRTs of 1.5 h, 1.0 h, and 0.5 h. The evaluation of bacteria number was accomplished by using the real-time PCR targeting ammonia monooxygenase (amoA) and 16S rDNA genes. In the absence of organic carbon in the influent, the shortening of HRT from 1.5 h to 0.5 h positively affected the AOB number in immobilized biomass, which reached even 30%. But their activity was limited. In the presence of organic carbon in the influent, the shortening of HRT below 1.5 h resulted in a decline of the abundance of ammonia-oxidizing bacteria and nitrification efficiency.

Wydawca

-

Rocznik

Tom

21

Numer

4

Opis fizyczny

p.1107-1112,fig.,ref.

Twórcy

autor
  • Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-709 Olsztyn, Poland

Bibliografia

  • 1. ANDREOTTOLA G., FOLADORI P., RAGAZZI M., TATANO F. Experimental comparison between MBBR and activated sludge system for the treatment of municipal wastewater. Water Sci. Technol. 41, 375, 2000.
  • 2. ZHANG B., SUN B., JI M., LIU H., LIU X. Quantification and comparison of ammonia-oxidizing bacterial communities in MBRs treating various types of wastewater. Bioresource Technol. 101, 3054, 2010.
  • 3. ROSTRON W.M., STUCKLEY D.C., YOUNG A.A. Nitrification of high strength ammonia wastewaters: comparative study of immobilisation media. Water Res. 35, 1169, 2001.
  • 4. XING X.H., JUN B.H., YANAGIDA M., TANJI Y., UNNO H. Effect of C/N values on microbial simultaneous removal of carbonaceous and nitrogenous substances in wastewater by single continuous-flow fluidized-bed biore- actor containing porous carrier particles. Biochem. Eng. J. 5, 29, 2000.
  • 5. KARIMINIAAE-HAMEDAANI H.R., KANDA K., KATO F. Wastewater treatment with bacteria immobilized onto a ceramic carrier in an aerated system. J. Biosci. Bioeng. 95, 128, 2003.
  • 6. MILNER M.G., CURTIS T.P., DAVENPORT R.J. Presence and activity of ammonia-oxidising bacteria detected amongst the overall bacterial diversity along a physico-chemical gradient of a nitrifying wastewater treatment plant. Water Res. 42, 2863, 2008.
  • 7. XIA S., LI J., WANG R., LI J., ZHANG Z. Tracking composition and dynamics of nitrification and denitrification microbial community in a biofilm reactor by PCR-DGGE and combining FISH with flow cytometry. Biochem. Eng. J. 49, 370, 2010.
  • 8. SIRIPONG S., RITTMANN B.E. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants. Water Res. 41, 1110, 2007.
  • 9. NOGUEIRA R., MELO L.F., PURKHOLD U., WUERTZ S., WAGNER M. Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon. Water Res. 36,469, 2002.
  • 10. ROWAN A.K., SNAPE J.R., FEARNSIDE D., BARER M.R., CURTIS T.P., HEAD I.M. Composition and diversity of ammonia-oxidizing bacterial communities in wastewater treatment reactors of different design treating identical wastewater. FEMS Microbiol. Ecol. 43, 195, 2003.
  • 11. KUO D.H.W., ROBINSON K.G., LAYTON A.C., MEYERS A.J., SAYLER G.S. Real-time PCR quantification of ammonia-oxidizing bacteria (AOB): solids retention time (SRT) impacts during activated sludge treatment of industrial wastewater. Environ. Eng. Sci. 23, 507, 2006.
  • 12. CYDZIK-KWIATKOWSKA A., WOJNOWSKA- BARYŁA I. The impact of organic carbon and ammonia load in wastewater on ammonia-oxidizing bacteria community in activated sludge. Pol. J. Microbiol. 57, 241, 2008.
  • 13. ZIELIŃSKA M., CYDZIK-KWIATKOWSKA A., WOJNOWSKA-BARYŁA I. The effect of some technological parameters on the diversity of immobilized microorganisms. Arch. Environ. Protect. 35, 45, 2009.
  • 14. ZIELIŃSKA M., WOJNOWSKA-BARYŁA I. Nitrification by biomass immobilized in porous carriers. J. Environ. Eng. Sci. 6, 463, 2007.
  • 15. COELHO M.A.Z., RUSSO C., ARAUJO O.Q.F. Optimization of sequencing batch reactor for biological nitrogen removal. Water Res. 34, 2809, 2000.
  • 16. APHA-AWWA-WEF. Standard methods for the examination of water and wastewater. 18th Edition. 1992.
  • 17. LANE D.J. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (Ed) Nucleic acid techniques in bacterial systematics. Wiley: Great Britain, pp. 205-248,1991.
  • 18. ROTTHAUWE J.H., WITZEL K.P., LIESACK W. The ammonia monooxygenase structural gene amoA as a ftinctional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl. Environ. Microb. 63, 4704,1997.
  • 19. OKANO Y., HRISTOVA K.R., LEUTENEGGER C.M., JACKSON L.E., DENISON R.F., GEBREYESUS B., LEBAUER D., SCOW K.M. Application of real-time PCR to study effects of ammonium on population size of ammonia-oxidizing bacteria in soil. Appl. Environ. Microb. 70, 1008, 2004.
  • 20. SUZUKI M.T., TAYLOR L.T., DELONG E.F. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays. Appl. Environ. Microb. 66, 4605, 2000.
  • 21. PHOLCHAN M.K., BAPTISTA J.C., DAVENPORT R.J., CURTIS T.P. Systematic study of the effect of operating variables on reactor performance and microbial diversity in laboratory-scale activated sludge reactors. Water Res. 44, 1341,2010.
  • 22. MORGENROTH E., OBERMAYER A., ARNOLD E., BRÜHL A., WAGNER M., WILDERER P.A. Effect of long-term idle periods on the performance of sequencing batch reactors. Water Sci. Technol. 41, 105, 2000.
  • 23. ZHANG B., SUN B., JI M., LIU H. Population dynamic succession and quantification of ammonia-oxidizing bacteria in a membrane bioreactor treating municipal wastewater. J. Hazard. Mater. 165, 796, 2009.
  • 24. GEETS J., COOMAN M., WITTEBOLLE L., HEYLEN K., VANPARYS B., DEVOS P., VERSTRAETE W., BOON N. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge. Appl. Microbiol. Biot. 75, 211, 2007.
  • 25. ZHU S., CHEN S. Effects of organic carbon on nitrification rate in fixed film biofilters. Aquacult. Eng. 25, 1, 2001.
  • 26. HANAKI K., WANTAWIN G., OHGAKI S. Effects of the activity of heterotrophs on nitrification in a suspendedgrowth reactor. Water Res. 24, 289, 1990.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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