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2014 | 70 | 01 |

Tytuł artykułu

Occurrence of Yersinia enterocolitica O:9 in feces from cows seropositive for brucellosis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The diagnosis of brucellosis is mainly based on serological tests. All animals classified as serologically positive are obligatorily slaughtered and subjected to bacteriological examination. B. abortus has not been reported in Poland since the eradication of bovine brucellosis in 1980. On the other hand, B. suis biovar 2 is sporadically isolated from cattle. In accordance with the instructions of the Chief Veterinary Officer, samples of feces from animals slaughtered following positive serological results for brucellosis have been examined for the presence of Yersinia enterocolitica O:9 since 2011. Because of the similarity of its O-polysaccharide component of S-LPS with that of Brucella, this microorganism is considered to be a major contributory factor of false positive serological reactions (FPSR). The paper presents the results of the bacteriological and molecular examination of feces from 26 cows seropositive for brucellosis and 30 healthy cows, negative for brucellosis, for the presence of Y. enterocolitica O:9. Y. enterocolitica O:9 was found in 7 of the positive cows, whereas all samples from cows negative for brucellosis were free from this bacteria. These results indicate that Y. enterocolitica O:9 may be responsible for some of the positive results for brucellosis in cattle.

Wydawca

-

Rocznik

Tom

70

Numer

01

Opis fizyczny

p.42-45,ref.

Twórcy

autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

  • 1. Annex no. 1 to Instruction no. GIWz.401/BB-16/2010 of the Chief Veterinary Officer, Warsaw 2011.
  • 2. Caroff M., Bundle D. R., Perry M. B.: Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9. Eur. J. Biochem. 1984, 139, 195-200.
  • 3. Commission Decision 2009/600/EC of 5 August 2009 amending Decision 2003/467/EC as regards the declaration that certain Member States and regions thereof are officially free of bovine brucellosis (OJ L204, 6.8.2009, pp. 39-42).
  • 4. Cook D. R., Noble J. W.: Isolation of Brucella suis from cattle. Aus. Vet. J. 1984, 61, 263-264.
  • 5. Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (OJ No. 121, 29.7.1964, pp. 1977/64).
  • 6. Ewalt D. R., Payeur J. P., Rhyan J. C., Geer P. L.: Brucella suis biovar 1 in naturally infected cattle: a bacteriological, serological and histological study. J. Vet. Diagn. Invest. 1997, 9, 417-420.
  • 7. Garin-Bastuji B., Hummel N., Gerbier G., Cau C., Pouillot R., Da Costa M., Fontaine J. J.: Nonspecific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O:9. Vet. Microbiol. 1999, 66, 223-233.
  • 8. Gerbier G., Garin-Bastuji B., Pouillot R., Very P., Cau C., Berr V., Dufour B., Moutou F.: False positive serological reactions in bovine brucellosis: evidence of the role of Yersinia enterocolitica serotype O:9 in a field trial. Vet. Res. 1997, 28, 375-383.
  • 9. Lubeck P. S., Skurnik M., Akrens P., Hoorfar J.: A Multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on perosamine syntethase gene. Adv. Exp. Med. Biol. 2003, 529, 451-455.
  • 10. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE, Paris, France 2010.
  • 11. Olsen S. C., Hennager S. G.: Immune response and Protection against Experimental Brucella suis Biovar 1 Challenge in Nonvaccinated or B. abortus Strain RB51-Vaccinated Cattle. Clin. Vaccine Immunol. 2010, 17, 1891-1895.
  • 12. Szulowski K., Iwaniak W., Pilaszek J.: Porcine brucellosis in Poland: problems accompanying serological surveys conducted in 1995-2000. Bull. Vet. Inst. Pulawy 2001, 45, 153-161.
  • 13. Szulowski K., Iwaniak W., Weiner M., Złotnicka J.: Brucella suis biovar 2 isolations from cattle in Poland. Ann. Agric. Environ. Med. 2013, vol. 20, no 4, 677-680.
  • 14. Wannet W. J. B., Reessink M., Brunings H. A., Maas H. M. E.: Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive Duplex PCR. J. Clin. Microbiol. 2001, 39, 4483-4486.
  • 15. Weiner M., Iwaniak W., Złotnicka J., Szulowski K.: Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarization assay. Bull. Vet. Inst. Pulawy 2010, 54, 485-488.
  • 16. Weiner M., Szulowski K., Iwaniak W.: Isolation of Yersinia enterocolitica O:9 from cows positive in serological examination of bovine brucellosis. Bull. Vet. Inst. Pulawy 2012, 56, 473-477.
  • 17. Weiner M., Szulowski K., Iwaniak W.: The porcine brucellosis – evidence of the role of Yersinia enterocolitica O:9 in occurence of false positive serological reactions. Polish J. Vet. Sci. 2013, 16, 129-130.
  • 18. Weynants V., Tibor A., Denoel P. A., Saegerman C., Godfroid J., Thiange P., Letesson J. J.: Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet. Microbiol. 1996, 48, 101-112.
  • 19. Wrathall A. E., Broughton E. S., Gill K. P., Goldsmith G. P.: Serological reactions to Brucella species in British pigs. Vet. Rec. 1993, 132, 449-454.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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