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2015 | 37 | 10 |
Tytuł artykułu

Molecular cloning and characterization of a pathogenesis-related protein SmPR10-1 from Salvia miltiorrhiza

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A full-length cDNA, SmPR10-1, encoding a pathogenesis-related class 10 protein, was isolated from Salvia miltiorrhiza, a well-known Chinese herbal plant. From the coding region of nucleotide sequence, the amino acid sequence can be predicted which contained conserved domain (K–A–X–E–X–Y) in the C-terminal helix found in most members of the PR10 protein family. Phylogenetic tree analysis indicated that the SmPR10-1 protein showed a sequence similarity which was much higher to dicot proteins than to other PR10 proteins. Prokaryotic expression of SmPR10-1 protein in fusion with a His-tag produced a 22 kDa protein in E. coli BL21 (DE3), which exhibited ribonuclease activity in vitro. The purified protein was used to assay antifungal activity and the results showed SmPR10-1 only inhibited the growth of Phytophthora infestans. The RT-PCR results showed that the SmPR10-1 was expressed in high transcript level in leaves, stalks and low levels in roots. This protein seems to involve in the plant active defense response through activation of the methyl jasmonate signaling pathway rather than salicylic acid or abscisic acid pathway.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
10
Opis fizyczny
Article: 205 [8 p.], fig.,ref.
Twórcy
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
  • School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
  • School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
autor
  • State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f3a8211e-2608-47b9-b2e1-3ef3bd1375db
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