PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 16 | 2 |
Tytuł artykułu

Dexamethasone, but not meloxicam, suppresses proliferation of bovine CD25plus CD4plus and CD25-CD4plus T cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Recently, we found that dexamethasone caused a depletion of CD25⁻ CD4⁺ T cells, but it increased the number of CD25highCD4⁺ and CD25lowCD4⁺ T cells. We also determined meloxicam-induced increase in the number of CD25highCD4⁺ T cells. In view of this, and taking into consideration the latest reports indicating that meloxicam shows an anti-proliferative effect on bovine peripheral blood mononuclear cells, it was considered purposeful to determine the effect of both drugs on proliferation of bovine CD25highCD4⁺, CD25lowCD4⁺ and CD25⁻ CD4⁺ T cells. Flow cytometry analysis and 5-bromo-2’-deoxyuridine incorporation assay were applied to detect the cell proliferation. It was demonstrated that dexamethasone, but not meloxicam, significantly reduced cell proliferation within all three evaluated CD4⁺ T cell subpopulations. Thus, the depletion of CD25⁻ CD4⁺ T cells by treatment with dexamethasone can partly be the effect of the anti-proliferative action of the drug, however, dexamethasone-induced increase in the number of CD25highCD4⁺ and CD25lowCD4⁺ T cells cannot be the result of enhanced proliferation of these cells.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
16
Numer
2
Opis fizyczny
p.219-222,fig.,ref.
Twórcy
autor
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland
Bibliografia
  • Blache C, Adriouch S, Calbo S, Drouot L, Dulauroy S, Arnoult C, Le Corre S, Six A, Seman M, Boyer O (2009) Cutting edge: CD4-independent development of functional FoxP3⁺ regulatory T cells. J Immunol 183: 4182-4186.
  • Chen X, Oppenheim JJ, Winkler-Pickett RT, Ortaldo JR, Howard OM (2006) Glucocorticoid amplifies IL-2-dependent expansion of functional FoxP3⁺CD4⁺CD25⁺ T regulatory cells in vivo and enhances their capacity to suppress EAE. Eur J Immunol 36: 2139-2149.
  • Maeda Y, Tanaka R, Ohtsuka H, Matsuda K, Tanabe T, Oikawa M (2011) Comparison of the immunosuppressive effects of dexamethasone, flunixin meglumine, and meloxicam on the in vitro response of calf peripheral blood mononuclear cells. J Vet Med Sci 73: 957-960.
  • Makita S, Kanai T, Oshima S, Uraushihara K, Totsuka T, Sawada T, Nakamura T, Koganei K, Fukushima T, Watanabe M (2004) CD4⁺CD25bright T cells in human intestinal lamina propria as regulatory cells. J Immunol 173: 3119-3130.
  • Maślanka T, Jaroszewski JJ (2012) In Vitro effects of dexamethasone on bovine CD25⁺CD4⁺ and CD25⁻CD4⁺ cells. Res Vet Sci 93: 1367-1379.
  • Maślanka T, Jaroszewski JJ. In Vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines and apoptosis of bovine CD25⁺CD4⁺ and CD25⁻CD4⁺ cells. J Vet Sci, in press.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f3a5e074-a820-4f38-b375-8ae609e61b93
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.