The effect of ensiling in round bales on the content of nitrogen fractions in lucerne and red clover protein

• Autorzy: Fijalkowska M. , Lipinski K. , Pysera B. , Wierzbowska J. , Antoszkiewicz Z. , Sienkiewicz S. , Stasiewicz M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine changes in nitrogen fractions during the ensiling of lucerne and red clover herbage in bales. Protein nitrogen and non-protein nitrogen compounds in herbage and silage were distinguished. Furthermore, buffer soluble nitrogen compounds (BSN) were determined and divided into buffer soluble protein and non-protein nitrogen (BSPN and NPBSN, respectively). In addition, peptide nitrogen and amino acid nitrogen were distinguished within the NPBSN fraction, while nitrogen compounds were divided into those of low solubility (NDIN) and completely insoluble (ADIN). The research findings confirmed the effect of a legume plant species on changes in the content of nitrogen fractions during the ensiling in round bales. The ensiling of lucerne herbage grossly decreased the protein nitrogen fraction while raising the total soluble fraction of nitrogen. Nitrogen in the form of soluble proteins, NDIN and ADIN was the least affected. The study demonstrated a different response of the two legume plant species to ensiling with respect to all fractions. A high proportion of protein nitrogen in round bale wilted red clover silage was accompanied by a large contribution of insoluble nitrogen fractions. A large share of the protein nitrogen fraction in red clover silage does not necessarily mean a higher nutritional value of the protein because it may contain a large amount of protein compounds that are non-hydrolysable by bacterial and intestinal enzymes.

Słowa kluczowe

EN

ensiling; round bale; nitrogen content; nitrogen fraction; lucerne; red clover; protein

• Wydawca: -
• Czasopismo: Journal of Elementology
• Rocznik: 2015
• Tom: 20
• Numer: 2
• Opis fizyczny: p.285-291,ref.

Twórcy

autor

Fijalkowska M.

• Chair of Animal Nutrition and Feed Science, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Lipinski K.

• Chair of Animal Nutrition and Feed Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Pysera B.

• Chair of Animal Nutrition and Feed Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Wierzbowska J.

• Chair of Agricultural Chemistry and Environment Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Antoszkiewicz Z.

• Chair of Animal Nutrition and Feed Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Sienkiewicz S.

• Chair of Agricultural Chemistry and Environment Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Stasiewicz M.

• Chair of Animal Nutrition and Feed Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

Bibliografia

• AOAC . 2005. Official Methods of Analysis. 15th ed. Assoc. Official. Anal. Chem. Washington. DC. USA
• Brzóska F., Pieszka M., Sala K. 1999. The effect of the dry matter content and fermentative additives on the chemical composition and protein breakdown of alfalfa silages. Rocz. Nauk. Zoot., 26(3): 231-242. (in Polish)
• Guo X.S., Ding W.R., Han J.G., Zhou H. 2008. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim. Feed Sci. Tech., 142(1-2): 89-98. DOI : :10.1016/j.anifeedsci.2007.07.005
• Givens D.I., Rulquin H. 2004. Utilization by ruminants of nitrogen compounds in silage-based diets. Anim. Feed Sci. Tech., 114(1-4): 1-18. DOI: 10.1016/j.anifeedsci.2003.09.005
• Hancock D.W., Coll ins M. 2006. Forage preservation method influences alfalfa nutritive value and feeding characteristics. Crop Sci., 46(2): 688-694.
• Hedqvist H., Udén P. 2006. Measurement of soluble protein degradation in the rumen. Anim. Feed Sci. Tech., 126 (1-2): 1-21. DOI: 10.1016/j.anifeedsci.2005.05.011
• Jones B.A., Muck R.E., Hatfield R.D. 1995. Red clover extracts inhibit legume proteolysis. J. Sci. Food Agric., 67(3): 329-333. DOI:10.1002/jsfa.2740670309
• Lee M. R. F., Scott M.B., Tweed J.K.S., Minchin F.R., Davies D.R. 2008. Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculant (Lactobacillus plantarum L54). Anim. Feed Sci. Tech., 144(1-2): 125-136. http://dx.doi. org/10.1016/j.anifeedsci.2007.09.035
• Licitra G., Hernandez T.M., Van Soest P.J. 1996. Standarization of procedures for nitrogen fractionation of ruminant feeds. Anim. Feed Sci. Tech., 57(4): 347-358.
• Nowak J. 2000. Influence of the type and moisture content of ensiling green fodder on round bale silage quality. Post. Nauk Rol., 5: 119-135. (in Polish)
• Owens V.N., Alb recht K.A., Muck R.E., Duke S.H. 1999. Protein degradation and fermentation characteristics of red clover and alfalfa silage harvested with varying levels of total non-structural carbohydrates. Crop Sci., 39(6): 1873-1880.
• Papadopoulos Y.A., McKersie B.D. 1983. A comparison of protein degradation during wilting and ensiling of six forage species. Can. J. Plant Sci., 63(4): 903-912.
• Purwin C., Łaniewska-Trokenheim Ł., Warmińska-Radyko I., Tywończuk J. 2006. The quality of silage - microbiological, health and production aspects. Med. Wet., 62(8): 865-869. (in Polish)
• Purwin C., Pysera B., Tokarczyk M., Sederevicius A., Savickis S., Traidaraitė A. 2009. Production results of dairy cows fed grass and alfalfa silage with a different degree of wilting. VetMed. Zoot., 46(68): 60-66.
• Purwin C., Pysera B., Sederevičius A., Makauskas S., Traidaraitė A., Lipiński K. 2010. Effect of silage made from different plant raw materials with the addition of fermentation inhibitor on the production results of dairy cows. Vet. Med. Zoot., 51(73): 44-51.
• Purwin C., Fijałkowska M., Pysera B., Lipiński K., Sienkiewicz S., Piwczyński D., Puzio N. 2014. Nitrogen fractions and amino acids content in alfalfa and red clover immediately after cutting and after wilting in the field. J. Elem., 19(3): 723-734. DOI: 10.5601/ jelem.2014.19.2.692
• Sull ivan M.L., Hatfield R.D. 2006. Polyphenol oxidase and o–diphenols inhibit postharvest proteolysis in red clover and alfalfa. Crop Sci., 46(2): 662-670. DOI:10.2135/cropsci2005.06-0132
• Thomas T.A. 1977. An automated procedure for the determination of soluble carbohydrates in herbage. J. Sci. Food Agric., 28(7): 639-642.
• Van Soest P.J., Robertson J.B., Lewis B.A. 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74(10): 3583-3597.
• Winters A.L., Fychan R., Jones R. 2001. Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance. Grass Forage Sci., 56 (2): 181-192.

Identyfikatory

DOI

10.5601/jelem.2014.19.4.643