Physiological and molecular characterization of salt response of Arabidopsis thaliana NOK2 ecotype

• Autorzy: Kaddour R. , M`rah S. , Karray-Bouraoui N. , Lambert C. , Berthomieu P. , Lachaal M.
• Języki publikacji: EN

Abstrakty

EN

Arabidopsis thaliana is a glycophyte capable to tolerate mild salinity. Although salt sensitivity of this species, a variability of this characteristic was revealed between different ecotypes. This study presents the physiological and molecular characteristics of salt response of two ecotypes, NOK2 and Columbia (Col). Seedlings were cultivated in hydroponics in the presence of 0 or 50 mM NaCl during 25 days. Rosette leaf samples were collected after 19, 22, and 25 days for determination of physiological parameters, and after 18 days for study of DNA polymorphism. Salt treatment decreased rosette dry matter, leaf number, leaf hydration, and leaf surface area. All these effects were significantly more visible in Col than in NOK2. Moreover, the NOK2 leaves accumulated less Na⁺ and more K⁺ than those of Col. DNA polymorphism between the two ecotypes was analyzed with codominant molecular markers based on PCR amplification, namely, microsatellites, cleaved amplified polymorphism sequence (CAPS), and single nucleotide polymorphism markers (SNP). Among the 35 tested markers, 17 showed a clear polymorphism and were distributed on the five Arabidopsis chromosomes ending with a genetic map construction. These results could play an important role in the future establishment of cartography of candidate gene controlling the K⁺/Na⁺ selectivity of ion transport in leaves, a component of plant salt tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 3
• Opis fizyczny: p.503-510,fig.,ref.

Twórcy

autor

Kaddour R.

• Physiologie et Biochimie de la Tole´rance au Sel des Plantes, Faculte´ des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

M`rah S.

• Physiologie et Biochimie de la Tole´rance au Sel des Plantes, Faculte´ des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Karray-Bouraoui N.

• Physiologie et Biochimie de la Tole´rance au Sel des Plantes, Faculte´ des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

autor

Lambert C.

• Biochimie & Physiologie Mole´culaire des Plantes, SupAgro/INRA, 34060 Montpellier Cedex 1, France

autor

Berthomieu P.

• Biochimie & Physiologie Mole´culaire des Plantes, SupAgro/INRA, 34060 Montpellier Cedex 1, France

autor

Lachaal M.

• Physiologie et Biochimie de la Tole´rance au Sel des Plantes, Faculte´ des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia

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