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2007 | 29 | 3 |
Tytuł artykułu

Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean (Phaseolus vulgaris L.)

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The most abundant form in which phosphorus occurs in seeds is phytate (myo-inositol hexakisphosphate), mostly known as an antinutrient for animals, given its ability to complex proteins and minerals, despite its antioxidant and anticarcinogenic properties. However, phytate synthesis is still poorly understood, both in terms of its regulation and metabolic route, and relatively few works have addressed the control mechanism of phytate accumulation during seed development. Aiming at understanding the control mechanism of phytate synthesis, we examined myo-inositol-3-phosphate synthase (MIPS) (EC 5.5.1.4) activity and gene expression during seed development of common bean. Phytate concentration was low at the initial stage of seed development, coinciding with a period of the most intense seed metabolism, but followed by a period of high enzymatic activity and gene expression of MIPS when a decrease in its specific activity and transcription was detected throughout seed development until 20 days after flowering; however, the specific activity of MIPS dropped more expressively than the gene expression, matching with higher phytate concentration. Hence, we show that there is evidence of one control point regulating phytate synthesis with MIPS enzyme.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
29
Numer
3
Opis fizyczny
p.265-271,fig.,ref.
Twórcy
  • Centro Agroveterinario, Universidade do Estado de Santa Catarina, Av. Luiz de Camoes, 2090, Lages, SC CEP 88520-000, Brazil
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
autor
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, SP, Brazil
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f26bae79-97b6-42bc-ba87-187e3e0de8b9
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