Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 29 | 3 |
Tytuł artykułu

Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean (Phaseolus vulgaris L.)

Warianty tytułu
Języki publikacji
The most abundant form in which phosphorus occurs in seeds is phytate (myo-inositol hexakisphosphate), mostly known as an antinutrient for animals, given its ability to complex proteins and minerals, despite its antioxidant and anticarcinogenic properties. However, phytate synthesis is still poorly understood, both in terms of its regulation and metabolic route, and relatively few works have addressed the control mechanism of phytate accumulation during seed development. Aiming at understanding the control mechanism of phytate synthesis, we examined myo-inositol-3-phosphate synthase (MIPS) (EC activity and gene expression during seed development of common bean. Phytate concentration was low at the initial stage of seed development, coinciding with a period of the most intense seed metabolism, but followed by a period of high enzymatic activity and gene expression of MIPS when a decrease in its specific activity and transcription was detected throughout seed development until 20 days after flowering; however, the specific activity of MIPS dropped more expressively than the gene expression, matching with higher phytate concentration. Hence, we show that there is evidence of one control point regulating phytate synthesis with MIPS enzyme.
Słowa kluczowe
Opis fizyczny
  • Centro Agroveterinario, Universidade do Estado de Santa Catarina, Av. Luiz de Camoes, 2090, Lages, SC CEP 88520-000, Brazil
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Centro Energia Nuclear na Agricultura, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, SP, Brazil
  • Abu-Abied M, Holland D (1994) Two newly isolated genes from citrus exhibit a different pattern of diurnal expression and light response. Plant Mol Biol 26:165–173
  • Barnett JEG, Brice RE, Corina DL (1970) A colorimetric determination of inositol monophosphate as an assay for D-glucose 6-phosphate-1L-myo-inositol 1-phosphate cyclase. Biochem J 119:183–186
  • Bernhart DN, Wreath AR (1955) Colorimetric determination of phosphorus by modified phosphomolybdate method. Anal Chem 27:440–441
  • Biswas S, Maiti SB, Chakrabarti S, Biswas BB (1978) Purification and characterization of myoinositol hexaphosphate adenosine diphosphate phosphotransferase from Phaseolus aureus. Arch Biochem Biophys 185:557–566
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein–dye binding. Anal Biochem 72:248–254
  • Brearley CA, Hanke DE (1996) Metabolic evidence for the order of addition of individual phosphate esters to the myoinositol moiety of inositol hexakisphosphate in the duckweed Spirodela polyrhiza L. Biochem J 314:227–233
  • Cheryan M (1980) Phytic acid interations in food systems. Crit Rev Food Sci Nutr 13:297–335
  • Coelho CMM, Santos JCP, Tsai SM, Vitorello VA (2002) Seed phytate content and phosphorus uptake and distribution in dry bean genotypes. Braz J Plant Physiol 14:51–58
  • Coelho CMM, Tsai SM, Vitorello VA (2005) Dynamics of inositolphosphate pools (tris-, tetrakis- and pentakisphosphate) in relation to the rate of phytate synthesis during seed development in common bean (Phaseolus vulgaris L.). J Plant Physiol 162:1–9
  • Fracs CCD, Thiellement H, Vienne DD (1985) Analysis of leaf proteins by two-dimensional gel electrophoresis. Plant Physiol 78:178–182
  • Hegeman CE, Good LL, Grabau EA (2001) Expression of D-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis. Plant Physiol 125:1941–1948
  • Johnson MD (1994) The Arabidopsis thaliana myo-inositol-1-phosphate synthase (EC Plant Physiol 105:1023–1024
  • Johnson MD, Wang X (1996) Differentially expressed forms of 1-L-myo-inositol-1-phosphate synthase (EC in Phaseolus vulgaris. J Biol Chem 271:17215–17218
  • Lackey KH, Pope PM, Johnson MD (2003) Expression of 1L-myoinositol-1-phosphate synthase in organelles. Plant Physiol 132:2240–2247
  • Latta M, Eskin M (1980) A simple method for phytate determination. J Agric Food Chem 28:1313–1315
  • Loewus FA, Murthy PPN (2000) Myo-inositol metabolism in plants. Plant Sci 150:1–19
  • Lonnerdal B (2002) Phytic acid-trace element (Zn, Cu, Mn) interactions. Int J Dev Biol 37:749–758
  • Lott JNA, Randal PJ, Goodchild DJ, Craig S (1985) Occurrence of globoid crystals in cotyledonary bodies of Pisum sativum as influenced changes in experimentally induced changes in Mg, Ca and K contents of seeds. Aust J Plant Physiol 12:341–353
  • Majerus PW, Connoly TM, Bansal VS, Inhorn RC, Ross TS, Lips D (1988) Inositol phosphates: synthesis and degradation (Minireview). J Biol Chem 263:3051–3054
  • Majumder AL, Biswas BB (1973) Metabolism of inositol phosphates: 5. Biosynthesis of inositol phosphates during ripening of mung bean (Phaseolus aureus) seeds. Indian J Exp Biol 11:120–123
  • Majumder AL, Chatterjee A, Dastigar KG, Majee M (2003) Diversification and evolution of L-myo-inositol 1-phosphate synthase. FEBS Lett 553:3–10
  • Nunes ACS, Cuneo F, Amaya-Farfán J, Capdeville Gd, Rech EL, Aragão FJL (2005) RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. Planta 223:45–51
  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2003–2007
  • Phillippy BQ (1998) Identification of inositol 1,3,4-trisphosphate 5-kinase and inositol 1,3,4,5-tetrakisphosphate 6-kinase in immature soybean seeds. Plant Physiol 116:291–297
  • Raboy V (1990) The biochemistry and genetics of phytic acid synthesis in higher plants. In: Morre EJ, Boss WS, Loewus FA (eds) Inositol metabolism in plants. Wiley, New York pp 55–76
  • Raboy V, Dickinson DB (1987) The timing and rate of phytic acid accumulation in developing soybean seeds. Plant Physiol 85:841–844
  • Raboy V, Noaman MM, Taylor GA, Pickett SG (1991) Grain phytic acid and protein are highly correlated in winter wheat. Crop Sci 31:631–635
  • Shi J, Wang H, Wu Y, Hazebroek J, Meeley RB, Ertl DS (2003) The maize low-phytic acid mutant lpa2 is caused by mutation in an inositol phosphate kinase gene. Plant Physiol 131:507–515
  • Shi J, Wang H, Hazebroek J, Ertl DS, Harp T (2005) The maize low-phytic acid three encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in development seed. Plant J 42:708–719
  • Sparvoli F, Fileppi M (2005) Isolation and characterization of the seed myo-inositol-3-phosphate synthase gene from common bean. Biologia e Biotecnologia Agrária, 20133
  • Stevenson-Paulik J, Odom AR, York JD (2002) Molecular and biochemical characterization of two plant inositol polyphosphate 6-/3-/5-kinases. J Biol Chem 277:42711–42718
  • Wilson MP, Majerus PW (1997) Characterization of a cDNA encoding Arabidopsis thaliana inositol 1,3,4-trisphosphate 5/6-kinase. Biochem Biophys Res Commun 232:678–681
  • Yoshida KT, Wada T, Koyama H, Mizobuchi-Fukuoka R, Naito S (1999) Temporal and spatial patterns of accumulation of the transcript of myo-inositol-1-phosphate synthase and phytin-containing particles during seed development in rice. Plant Physiol 119:65–72
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.