Phenotyping of transgenic mice: comparison of intellicage-system to automatic monitoring of behavior in social context with standard tests

• Autorzy: Kiryk A. , Kaczmarek L.
• Języki publikacji: EN

Abstrakty

EN

There is a growing need for new strategies allowing for screening, monitoring and phenotyping of genetically modifi ed animals. One of the instruments is IntelliCage system, an automated testing device allowing to investigate behavior in social groups of mice in repetitive conditions, without excessive contact with experimenter. We compared results of 4 strains of genetically modifi ed mice tested individually in standard battery of tests with the animal performance in social context in IntelliCage. The overall behavior of tested strains, namely D2 knock-out mice lacked adult neurogenesis, GLT1 knock-out mice deprived of glial glutamate transporter, APP.V717I transgenic mice expressing the mutant human amyloid precursor protein and Dicer mice with forebrain specifi c and inducible inactivation of Dicer gene, were at fi rst analyzed in sensorimotor tests, open fi eld and elevated plus maze. Activity, exploration and anxiety level were then investigated in IntelliCage system and we found that all the tests were able to detect differences in phenotype. Furthermore, learning and memory tests were conducted in IntelliCage, e.g. place preference and confronted with traditional learning tasks, such as Morris water maze. Again, IntelliCage as well as standard tests differentiated mice with mutation from their wild-type siblings. We also found on example of APP.V717I transgenic mice prospects for investigation the effect of the social interaction on learning in IntelliCage system.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.371

Twórcy

autor

Kiryk A.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Kaczmarek L.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland