U937 variant cells as a model of apoptosis without cell disintegration

• Autorzy: Stasilojc G. , Pinto S. , Wyszkowska R. , Wejda M. , Slominska E. M. , Filipska M. , Koszalka P. , Swierczynski J. , O`Connor J. E. , Bigda J. J.
• Języki publikacji: EN

Abstrakty

EN

The variant cell line U937V was originally identified by a higher sensitivity to the cytocidal action of tumor necrosis factor alpha (TNFα) than that of its reference cell line, U937. We noticed that a typical morphological feature of dying U937V cells was the lack of cellular disintegration, which contrasts to the formation of apoptotic bodies seen with dying U937 cells. We found that both TNFα, which induces the extrinsic apoptotic pathway, and etoposide (VP-16), which induces the intrinsic apoptotic pathway, stimulated U937V cell death without cell disintegration. In spite of the distinct morphological differences between the U937 and U937V cells, the basic molecular events of apoptosis, such as internucleosomal DNA degradation, phosphatidylserine exposure on the outer leaflet of the plasma membrane, caspase activation and cytochrome c release, were evident in both cell types when stimulated with both types of apoptosis inducer. In the U937V cells, we noted an accelerated release of cytochrome c, an accelerated decrease in mitochondrial membrane potential, and a more pronounced generation of reactive oxygen species compared to the reference cells. We propose that the U937 and U937V cell lines could serve as excellent comparison models for studies on the mechanisms regulating the processes of cellular disintegration during apoptosis, such as blebbing (zeiosis) and apoptotic body formation.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2013
• Tom: 18
• Numer: 2
• Opis fizyczny: p.249-262,fig.,ref.

Twórcy

autor

Stasilojc G.

• Department of Cell Biology, Intercollegiate Faculty of Technology, UG-MUG, Medical University of Gdansk, Poland

autor

Pinto S.

autor

Wyszkowska R.

autor

Wejda M.

autor

Slominska E. M.

autor

Filipska M.

autor

Koszalka P.

autor

Swierczynski J.

autor

O`Connor J. E.

autor

Bigda J. J.

Bibliografia

• 1. Viorritto, I.C.B., Nikolov, N.P. and Siegel, R.M. Autoimmunity versus tolerance: can dying cells tip the balance? Clin. Immunol. 122 (2007) 125-134.
• 2. Gurumurthy, S., Vasudevan, K.M. and Rangnekar, V.M. Regulation of apoptosis in prostate cancer. Cancer Metastasis Rev. 20 (2001) 225-243.
• 3. Böhm, I. and Schild, H. Apoptosis: the complex scenario for a silent cell death. Mol. Imaging Biol. 5 (2003) 2-14.
• 4. Bras, M., Queenan, B. and Susin, S.A. Programmed cell death via mitochondria: different modes of dying. Biochemistry (Mosc) 70 (2005) 231-239.
• 5. Krysko, D.V., VandenBerghe, T., D’Herde, K. and Vandenabeele, P. Apoptosis and necrosis: detection, discrimination and phagocytosis. Methods 44 (2008) 205-221.
• 6. Oliveira, J.B. and Gupta, S. Disorders of apoptosis: mechanisms for autoimmunity in primary immunodeficiency diseases. J. Clin. Immunol. 28 Suppl. 1, (2008) S20-8.
• 7. Jana, N.R. NSAIDs and apoptosis. Cell Mol. Life Sci. 65 (2008) 1295-1301.
• 8. Festjens, N., VandenBerghe, T. and Vandenabeele, P. Necrosis, a wellorchestrated form of cell demise: signalling cascades, important mediators and concomitant immune response. Biochim. Biophys. Acta 1757 (2006) 1371-1387.
• 9. Groninger, E., Meeuwsen-De Boer, G.J., De Graaf, S.S.N., Kamps, W.A. and De Bont, E.S.J.M. Vincristine induced apoptosis in acute lymphoblastic leukaemia cells: a mitochondrial controlled pathway regulated by reactive oxygen species? Int. J. Oncol. 21 (2002) 1339-1345.
• 10. Liao, P. and Lieu, C. Cell cycle specific induction of apoptosis and necrosis by paclitaxel in the leukemic U937 cells. Life Sci. 76 (2005) 1623-1639.
• 11. Cerella, C., Scherer, C., Cristofanon, S., Henry, E., Anwar, A., Busch, C., Montenarh, M., Dicato, M., Jacob, C. and Diederich, M. Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4). Apoptosis 14 (2009) 641-654.
• 12. Van Hoof, C. and Goris, J. Phosphatases in apoptosis: to be or not to be, PP2A is in the heart of the question. Biochim. Biophys. Acta 1640 (2003) 97-104.
• 13. Hail, N.J. Mitochondria: A novel target for the chemoprevention of cancer. Apoptosis 10 (2005) 687-705.
• 14. Hori, T., Kondo, T., Tabuchi, Y., Takasaki, I., Zhao, Q., Kanamori, M., Yasuda, T. and Kimura, T. Molecular mechanism of apoptosis and gene expressions in human lymphoma U937 cells treated with anisomycin. Chem. Biol. Interact. 172 (2008) 125-140.
• 15. Ho, S., Chen, W., Chiu, H., Lai, C., Guo, H. and Wang, Y. Combination treatment with arsenic trioxide and irradiation enhances apoptotic effects in U937 cells through increased mitotic arrest and ROS generation. Chem. Biol. Interact. 179 (2009) 304-313.
• 16. Kaszubowska, L., Engelmann, H., Gotartowska, M., Iliszko, M. and Bigda, J. Identification of two U937 cell sublines exhibiting different patterns of response to tumour necrosis factor. Cytokine 13 (2001) 365-370.
• 17. Shin, D.Y., Kim, G.Y., Li, W., Choi, B.T., Kim, N.D., Kang, H.S. and Choi, Y.H. Implication of intracellular ROS formation, caspase-3 activation and Egr-1 induction in platycodon D-induced apoptosis of U937 human leukemia cells. Biomed. Pharmacother. 63 (2009) 86-94.
• 18. Python, F., Goebel, C. and Aeby, P. Assessment of the U937 cell line for the detection of contact allergens. Toxicol. Appl. Pharmacol. 220 (2007) 113-124.
• 19. Denecker, G., Vercammen, D., Declercq, W. and Vandenabeele, P. Apoptotic and necrotic cell death induced by death domain receptors. Cell Mol. Life Sci. 58 (2001) 356-370.
• 20. Takahashi, A., Masuda, A., Sun, M., Centonze, V.E. and Herman, B. Oxidative stress-induced apoptosis is associated with alterations in mitochondrial caspase activity and Bcl-2-dependent alterations in mitochondrial pH (pHm). Brain Res. Bull. 62 (2004) 497-504.
• 21. Smart, D.J., Halicka, H.D., Schmuck, G., Traganos, F., Darzynkiewicz, Z. and Williams, G.M. Assessment of DNA double-strand breaks and gammaH2AX induced by the topoisomerase II poisons etoposide and mitoxantrone. Mutat. Res. 641 (2008) 43-47.
• 22. Sundstrom, C. and Nilsson, K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17 (1976) 565-577.
• 23. Ndozangue-Touriguine, O., Hamelin, J. and Bréard, J. Cytoskeleton and apoptosis. Biochem. Pharmacol. 76 (2008) 11-18.
• 24. Izyumov, D.S., Avetisyan, A.V., Pletjushkina, O.Y., Sakharov, D.V., Wirtz, K.W., Chernyak, B.V. and Skulachev, V.P. "Wages of fear": transient threefold decrease in intracellular ATP level imposes apoptosis. Biochim. Biophys. Acta 1658 (2004) 141-147.
• 25. Orlando, K.A., Stone, N.L. and Pittman, R.N. Rho kinase regulates fragmentation and phagocytosis of apoptotic cells. Exp. Cell Res. 312 (2006) 5-15.
• 26. Salvioli, S., Barbi, C., Dobrucki, J., Moretti, L., Pinti, M., Pedrazzi, J., Pazienza, T.L., Bobyleva, V., Franceschi, C. and Cossarizza, A. Opposite role of changes in mitochondrial membrane potential in different apoptotic processes. FEBS Lett. 469 (2000) 186-190.
• 27. Chung, Y.M., Bae, Y.S. and Lee, S.Y. Molecular ordering of ROS production, mitochondrial changes, and caspase activation during sodium salicylate-induced apoptosis. Free Radic. Biol. Med. 34 (2003) 434-442.
• 28. Watabe, M. and Nakaki, T. ATP depletion does not account for apoptosis induced by inhibition of mitochondrial electron transport chain in human dopaminergic cells. Neuropharmacology 52 (2007) 536-541.
• 29. Krysko, D.V., D'Herde, K. and Vandenabeele, P. Clearance of apoptotic and necrotic cells and its immunological consequences. Apoptosis 11 (2006) 1709-1726.
• 30. Raina, A.K., Hochman, A., Zhu, X., Rottkamp, C.A., Nunomura, A., Siedlak, S.L., Boux, H., Castellani, R.J., Perry, G. and Smith, M.A. Abortive apoptosis in Alzheimer's disease. Acta Neuropathol. 101 (2001) 305-310.
• 31. Schrijvers, D.M., Martinet, W., De Meyer, G.R.Y., Andries, L., Herman, A.G. and Kockx, M.M. Flow cytometric evaluation of a model for phagocytosis of cells undergoing apoptosis. J. Immunol. Methods 287 (2004) 101-108.
• 32. Petrovski, G., Zahuczky, G., Katona, K., Vereb, G., Martinet, W., Nemes, Z., Bursch, W. and Fésüs, L. Clearance of dying autophagic cells of different origin by professional and non-professional phagocytes. Cell Death Differ. 14 (2007) 1117-1128.

Uwagi

rekord w opracowaniu