Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China

• Autorzy: Li R.-f. , Tian X.-g. , Liu Y. , Xu J. , Liu D.-y.
• Języki publikacji: EN

Abstrakty

EN

Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 1
• Opis fizyczny: p.65-73,fig.,ref.

Twórcy

autor

Li R.-f.

• College of Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China

autor

Tian X.-g.

• Department of Respiration, Central Hospital of Zhoukou city, Zhoukou 466000, China

autor

Liu Y.

• Department of Respiration, Central Hospital of Zhoukou city, Zhoukou 466000, China

autor

Xu J.

• Medical Research Center, Xinxiang Medical College, Xinxiang 453003, China

autor

Liu D.-y.

• College of Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China

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Identyfikatory

DOI

10.1515/pjvs-2016-0009