The role of the sympathecic nervous system in amphetamine-induced modulation of natural killer cytotoxicity in rats

• Autorzy: Glac W. , Badtke P. , Orlikowska A. , Tokarski J. , Wrona D.
• Języki publikacji: EN

Abstrakty

EN

It is well known that the main site of the action of amphetamine (AMPH) are the catecholaminergic neurons located both in central nervous system and in the sympathetic nervous system (SNS). To analyse the potential role of the SNS in the mechanism of AMPHinduced changes in natural killer cells cytotoxicity (NKCC) rats were sympathectomized by 6-hydroxydopamine (6-OHDA, 3 × 50 mg/kg, i.p.) prior to AMPH (1 mg/kg, i.p.) administration. In a separate experiment, rats were pretreated with a α-adrenergic receptor antagonist phentolamine (5 mg/kg, i.p.), β-adrenergic receptor antagonist propranolol (5 mg/kg, i.p.) or both. NKCC (51Cr-release assay) and the number of LGL (NK cells) were evaluated in the peripheral blood and spleen. In the peripheral blood AMPH-induced stimulation of NKCC was completely blocked by 6-OHDA. The increase in LGL number in the peripheral blood evoked by AMPH was partially inhibited by sympathectomy. In the spleen both effects of AMPH i.e. reduction of NKCC and decrease in LGL number were completely reversed by 6-OHDA. β-antagonist attenuated the AMPH-induced changes in NKCC and LGL number in the peripheral blood and spleen. In contrast, changes in NKCC and LGL number were not affected by α-blockade. These data clearly show that both AMPH-induced stimulation of the peripheral blood NKCC and suppression of spleen NKCC are mediated by the SNS. Furthermore, catecholamines elevated by AMPH modulate the NKCC via β-adrenergic, but not α-adrenergic mechanism.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.350

Twórcy

autor

Glac W.

• Department of Animal Physiology, University of Gdansk, Gdansk, Poland

autor

Badtke P.

• Department of Animal Physiology, University of Gdansk, Gdansk, Poland

autor

Orlikowska A.

• Department of Animal Physiology, University of Gdansk, Gdansk, Poland

autor

Tokarski J.

• Department of Animal Physiology, University of Gdansk, Gdansk, Poland

autor

Wrona D.

• Department of Animal Physiology, University of Gdansk, Gdansk, Poland