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2007 | 29 | 3 |
Tytuł artykułu

In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An efficient, rapid and reproducible plant regeneration protocol was successfully developed for Cassia angustifolia using nodal explants excised from 14-day-old aseptic seedlings. Of the two cytokinins, 6-benzyladenine (BA) and thidiazuron (TDZ) evaluated as supplements to Murashige and Skoog (MS) medium, TDZ at an optimal concentration of 5.0 µM was effective in inducing multiple shoots. The highest rate of shoot multiplication was achieved on MS medium supplemented with 5.0 µM TDZ and 1.0 µM indole-3-acetic acid (IAA) at pH 5.8. The regenerated shoots when subcultured on hormone free MS medium considerably increased the rate of shoot multiplication and shoot length by end of fourth subculture passage. Rooting was achieved on the isolated shoots using MS medium with 60 µM indole -3- butyric acid (IBA) and 1% activated charcoal for 1 week and subsequently transferring the shootlets to half strength MS liquid media without IBA and activated charcoal. The in vitro raised plantlets with welldeveloped shoot and roots were successfully established in earthen pots containing garden soil and grown in greenhouse.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
29
Numer
3
Opis fizyczny
p.233-238,fig.,ref.
Twórcy
autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India
autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India
Bibliografia
  • Agrawal V, Sardar PR (2003) In vitro organogenesis and histomorphological investigations in Senna (Cassia angustifolia)—a medicinally valuable shrub. Physiol Mol Biol Plants 9:131–140
  • Agrawal V, Sardar PR (2006) In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli. Biol Plant 50:118–122
  • Ajithkumar D, Seeni S (1998) Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep 17:422–426
  • Anis M, Husain MK, Shahzad A (2005) In vitro plantlet regeneration of Pterocarpus marsupium Roxb., an endangered leguminous tree. Curr Sci 88:861–863
  • Anonymous (1992) The wealth of India: a dictionary of Indian raw materials and industrial products, vol 3. CSIR, New Delhi, pp 354–363
  • Caboni ED, Angeli S, Chiappetta A, Innocenti AM, Van Onckelen H, Damiano C (2002) Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process. Plant Cell Tissue Organ Cult 70:199–206
  • Faisal M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Biol Plant 50:437–440
  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80:187–190
  • Feyissa T, Welander M, Negash L (2005) Micropropagation of Hagenia abyssinica (Bruce) J.F. Gmel: a multipurpose tree. Plant Cell Tissue Organ Cult 80:119–127
  • Franz G (1993) The senna drug and its chemistry. Pharmocology 47:2–6
  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119
  • Kanyand M, Peterson CM (1997) The differentiation of emergences into adventitious shoots in peanut, Arachis hypogaea (L.). Plant Sci 126:87–95
  • Koroch K, Juliani HP, Kapteyn J, Simon JE (2003) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79–83
  • Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L. high frequency induction of direct shoot formation in intact seedlings by N6-benzyl aminopurine and thidiazuron. Planta 186:384–389
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
  • Murthy BNS, Victor J, Singh RPS, Fletcher RA, Saxena PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regul 19:233–240
  • Parliman BJ, Evans PT, Rupert EA (1982) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex. L., the venus fly trap for rapid asexual propagation. HortScience 107:305–310
  • Pence VC (1999) The application of biotechnology for the conservation of endangered plants. In: Benson EE (ed) Plant conservation biotechnology, vol 15. Taylor & Francis, London, pp 227–241
  • Shailendra V, Joshi N, Tak K, Purohit SD (2005) In vitro adventitious shoot bud differentiation and plantlet regeneration in Feronia limonia L. (swingle). In Vitro Cell Dev Biol Plant 41:296–302
  • Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult 66:9–16
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f012d8cb-ff15-4f24-a75e-e737bfb6cbc4
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