Adventitious bud regeneration from leaf explants of Platanus occidentalis L. and genetic stability assessment

• Autorzy: Sun Y. , Zhao Y. , Wang X. , Qiao G. , Chen G. , Yang Y. , Zhou J. , Jin L. , Zhuo R.
• Języki publikacji: EN

Abstrakty

EN

The aim of this work is to develop a method of plant regeneration from leaf explants of Platanus occidentalis L. successfully. Woody plant medium (HortScience 16:453–459, 1981) and Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium were used as induced and rooted basal medium, respectively. The effects of combinations of 6-BA, IBA, NAA and KT with different concentrations on adventitious bud regeneration from P. occidentalis leaf explants were compared. The results showed that the highest shoot regeneration frequency (90%) and maximum number (13.72 ± 0.44) of shoots per explant was recorded on WPM medium supplemented with 22.20 mmol l⁻¹ 6-BA and 0.49 mmol l⁻¹ IBA. A 40-day-old explants were much more productive for shoot formation than others in this study. The regenerated shoots were cultured on MS medium supplemented with 1.33 mmol l⁻¹ 6-BA, 0.16 mmol l⁻¹ NAA and 2% (w/v) adenine, after 2-week shoots were transferred to 1/2 MS medium supplemented with 0.49 mmol l⁻¹ IBA for rooting. Hardened plantlets via acclimatization were transferred to pots and transplanted to the soil finally. To ascertain whether tissue culture had effects on the genetic stability of plantlets regenerated, the genetic diversity was assessed using RAPD marker. A total of 96 bands ranging from 0.5 to 2.2 kb with an average of 6.4 bands per primer, were obtained using 15 primers. Amplified products exhibited few of polymorphic patterns across all the plants of P. occidentalis and the overall frequency of detection of somaclonal polymorphisms was lower than 0.0104%.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 1
• Opis fizyczny: p.33-41,fig.,ref.

Twórcy

autor

Sun Y.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China
• College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China

autor

Zhao Y.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China

autor

Wang X.

• College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China

autor

Qiao G.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China

autor

Chen G.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China
• College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China

autor

Yang Y.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China

autor

Zhou J.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China

autor

Jin L.

• College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China

autor

Zhuo R.

• Key Lab of Tree Genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang, Hongzhou, Zhejiang 311400, China

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