Effect of salt stress on growth parameters, enzymatic antioxidant system, and lipid peroxidation in wild chicory (Cichorium intybus L.)

• Autorzy: Sergio L. , De Paola A. , Cantore V. , Pieralice M. , Cascarano N. A. , Bianco V. V. , Di Venere D.
• Języki publikacji: EN

Abstrakty

EN

Efficient utilization of saline land for food cultivation can increase agricultural productivity and rural income. To obtain information on the salt tolerance/susceptibility of wild chicory (Cichorium intybus L.), the influence of salinity (0–260 mM NaCl) on chicory seed germination and that of two salinity levels of irrigationwater (100 and 200 mMNaCl) on plant growth, antioxidative enzyme activity, and accumulation of proline and malondialdehyde (MDA) were investigated. The trials were performed outdoors, in pots placed under a protective glass covering, for two consecutive years. Seeds showed a high capacity to germinate in saline conditions. The use of 100 mM NaCl solution resulted in 81 % germination, whereas seed germinability decreased below 40 % using salt concentrations above 200 mM NaCl. Wild chicory showed tolerance to medium salinity (100 mM NaCl), whereas a drastic reduction in biomass was observed when 200 mM NaCl solution was used for irrigation. MDA, present in higher amounts in leaves than in roots, decreased in both tissues under increasing salinity. Proline content increased remarkably with the level of salt stress, more so in roots than in leaves. In salt stress conditions, the activity of antioxidant enzymes (APX, CAT, POD, SOD)was enhanced. The electrophoretic patterns of the studied enzymes showed that the salinity of irrigation water affected only the intensity of bands, but did not activate new isoforms. Our results suggest that wild chicory is able to grow in soil with moderate salinity by activating antioxidative responses both in roots and leaves.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2349-2358,fig.,ref.

Twórcy

autor

Sergio L.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

autor

De Paola A.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

autor

Cantore V.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

autor

Pieralice M.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

autor

Cascarano N. A.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

autor

Bianco V. V.

• Departamento of Sciences of Plant Production (DSPV), University of Bari "Aldo Moro", Via Amendola 165/A, 70126 Bari, Italy

autor

Di Venere D.

• CNR, Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, 70126 Bari, Italy

Bibliografia

• Acevedo A, Paleo AD, Federico ML (2001) Catalase deficiency reduces survival and pleiotropically affects agronomic performance in field-grown barley progeny. Plant Sci 160:847–855
• Aebi H (1974) Catalase, in: Bergmeyer H (ed.) Methods of Enzymatic Analysis. Academic Press, New York 2:673–684
• Arshi A, Abdin MZ, Iqbal M (2006) Effect of CaCl₂ on growth performance, photosynthetic efficiency and nitrogen assimilation of Cichorium intybus L. grown under NaCl stress. Acta Physiol Plant 28:137–147
• Arshi A, Ahmad A, Aref IM, Iqbal M (2010) Effect of calcium against salinity-induced inhibition in growth, ion accumulation and proline contents in Cichorium intybus L. J Environ Biol 31:939–944
• Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
• Bergamo P, Fedele E, Balestrieri M, Abrescia P, Ferrara L (1998) Measurement of malondialdehyde levels in food by highperformance liquid chromatography with fluorometric detection. J Agric Food Chem 46:2171–2176
• Beyer W, Fridovich I (1987) Assaying for superoxide dismutase activity; some large consequences of minor changes in condition. Anal Biochem 161:559–566
• Bianco VV (2009) Wild chicory: folklore, valorization, food, officinal and ornamental use. Ital J Agron Riv Agron 4 (4 Suppl):143–151
• Bor M, Özdemir F, Türkan I (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Sci 164:77–84
• Bradford MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–253
• Breusegem FV, Vranova E, Dat JF, Inze D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414
• Conti F, Abbate G, Alessandrini A, Blasi C (2005) An annotated checklist of the Italian vascular flora. Palombi (ed.), Rome Cucci G, De Caro A, Ciciretti L, Leoni B (1994) Salinity and germination of some vegetable crops. Acta Hortic 362:305–309
• Davey MW, Stals E, Panis B, Keulemans J, Swennen RL (2005) High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 347:201–207
• Di Venere D, Sergio L, Linsalata V, Pieralice M, Cardinali A, Cascarano N, Bianco VV (2009) Antioxidant properties of wild edible herbaceous species. Ital J Agron Riv Agron 4(4 Suppl):635–640
• Du Z, Bramlage WJ (1992) Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40:1566–1570
• Ellouzi H, Hamed KB, Cela J, Munné-Bosch S, Abdelly C (2011) Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Physiol Plant 142:128–143
• Eraslan F, Inal A, Savasturk O, Gunes A (2007) Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity. Sci Hort 114:5–10
• Gossett DR, Millhollon EP, Lucas MC (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34:706–714
• Gossett DR, Banks SW, Millhollon EP, Lucas MC (1996) Antioxidant responses to NaCl stress in a control and NaCl-tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine and exogenous glutathione. Plant Physiol 112:803–809
• Grassmann J, Hippeli S, Elstner EF (2002) Plant’s defence and its benefits for animals and medicine: role of phenolics and terpenoids in avoiding oxygen stress. Plant Physiol Biochem 40:471–478
• Hamilton EW, Heckathorn SA (2001) Mitochondrial adaptations to NaCl. Complex I is protected by anti-oxidants and small heat shock Proteins, whereas complex II is protected by proline and betaine. Plant Physiol 126:1266–1274
• Hasegawa PH, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
• Heimler D, Isolani L, Vignolini P, Romani A (2009) Polyphenol content and antiradical activity of Cichorium intybus L. from biodynamic and conventional farming. Food Chem 114:765–770
• Jin X, Huang Y, Zeng F, Zhou M, Zhang G (2009) Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley. Acta Physiol Plant 31:1103–1109
• Katsuhara M, Otsuka T, Ezaki B (2005) Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis. Plant Sci 169:369–373
• Kavi Kishor PB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRSS, Rao Sreenath Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
• Kim DS, Lee IS, Jang CS, Kang SY, Park IS, Song HS, Seo YW (2005) High amino acid accumulating 5-methyltryptophanresistant rice mutants may include an increased antioxidative response system. Physiol Plant 123:302–313
• Koca H, Bor M, Özdemir F, Türkan I (2007) The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ Exp Bot 60:344–351
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the hand of bacteriophage. Nature 22:680–685
• Lebeda A, Dolezal K (1995) Peroxidase isozyme polymorphism as a potential marker for detection of field resistance in Cucumis sativus to cucumber downy mildew, Pseudoperonospora cubensis Berk. et Curt. Rostov. J Plant Dis Prot 102(5):467–471
• Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160(10):1157–1164
• Magné C, Larher F (1992) High sugar content of extracts interferes with colorimetric determination of amino acids and free proline. Anal Biochem 200:115–118
• Meloni DA, Olive MA, Martinaze CA, Cambaia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76
• Miszalski Z, Slesak I, Niewiadomska E, Baczek-Kwinta R, Lüttge U, Ratajczak R (1998) Subcellular localization and stress response of superoxide dismutase isoforms from leaves in the C3-CAM intermediate halophyte Mesembryanthemum crystallinum L. Plant, Cell Environ 21:169–179
• Mittler R, Zilinskas B (1993) Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Anal Biochem 212:540–546
• Mittova V, Tal M, Volokita M, Guy M (2002) Salt stress induces upregulation of an efficient chloroplast antioxidant system in the salt-tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiol Plant 115:393–400
• Mittova V, Guy M, Tal M, Volokita M (2004) Salinity upregulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J Exp Bot 55:1105–1113
• Möller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu Rev Plant Physiol Plant Mol Biol 52:561–591
• Muckenschnabel I, Goodman BA, Williamson B, Lyon GD, Deighton N (2002) Infection of leaves of Arabidopsis thaliana by Botrytis cinerea: changes in ascorbic acid, free radicals and lipid peroxidation products. J Exp Bot 53:207–214
• Neel JPS, Alloush GA, Belesky DP, Clapham WM (2002) Influence of rhizosphere ionic strength on mineral composition, dry matter yield and nutritive value of forage chicory. J Agron Crop Sci 188:398–407
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
• Ruiz JM, Blasco B, Rivero RM, Romero L (2005) Nicotine-free and salt tolerant tobacco plants obtained by grafting to salinityresistant rootstocks of tomato. Physiol Plant 124:465–475
• Sehmer L, Alaoui-Sosse B, Dizengremel P (1995) Effect of salt stress on growth and on the detoxifying pathway of peduncolate oak seedlings (Quercus robur L.). J Plant Physiol 147:144–151
• Sun Y, Elwell JH, Oberley LW (1988) A simultaneous visualization of the antioxidant enzymes glutathione peroxidase and catalase on polyacrylamide gel. Free Radic Res Commun 5:67–75
• Tomiyama K, Stahmann MA (1964) Alteration of oxidative enzymes in potato tuber tissue by infection with Phytophtora infestans. Plant Physiol 39:483–490
• Tuteja N (2007) Mechanisms of high salinity tolerance in plants. Methods Enzymol 428:419–438
• Zushi K, Matsuzoe N (2009) Seasonal and cultivar differences in saltinduced changes in antioxidant system in tomato. Sci Hortic 120:181–187

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