Focal cortical stroke in the cat visual cortex

• Autorzy: Szczerba M. , Urban P. , Foik A. , Kublik E. , Ghazaryar A. , Borowska J. , Sadowska M. , Jablonka J. , Waleszczyk W.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUNS AND AIMS: The study develops methodology of photothrombotic stroke (PtS) induction in cats visual cortex for future studies of post-stroke visual recovery. Considering its well known organisation and similarity to the human, cat visual system is a good model for spontaneous and supported brain reorganisation after ischemia. METHODS: Photothrombosis was used as a model of focal ischemic cortical stroke. Standardisation of the methodology of cortical PtS was performed on four experimental cats with parallel local field potential (LFP) recordings in and around the stroke core – before, during and after the infarct. Intravenously injected Bengal Rose was locally irradiated by cold light via an optic bundle placed on the skull, thinned skull or directly on dura surface. Different light source parameters and irradiation time were tested. Postoperatively isolated brains were preserved and frozen, cut and stained. Final position of the electrodes was monitored on dried 50 μm slices. The cytochrome oxidase (CO) activity and Nissl staining were used to monitor the state of the tissue injury. RESULTS: The aimed unilateral stroke was performed in the dorsal zone of the left marginal gyrus over the visual cortex on a border of the cortical areas 17 and 18. CO visualizes the areas of lower mitochondrial activity in the illuminated tissue of the irradiated cortex in comparison to the contralateral intact homotopic areas. The spontaneous LFP dynamic decreased for at least three hours within the irradiated cortex but not in opposite hemisphere or surrounding tissue. CONCLUSIONS: The 25 minutes of the unilateral irradiation directly to the dura surface with the light temperature 2750K resulted in the most accurate lesion covering all the width of the marginal gyrus and partially the sulcus area not spreading on adjacent gyri or further blood vessels. The infarct reaches the white matter without its pronounced injury. Supported by:ERA-NET:REVIS&MNiSW0292/IP1/2013/72.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S97

Twórcy

autor

Szczerba M.

• Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Urban P.

• Faculty of Physics, University of Warsaw, Warsaw, Poland

autor

Foik A.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences Warsaw, Poland, Warsaw, Poland

autor

Kublik E.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences Warsaw, Poland, Warsaw, Poland

autor

Ghazaryar A.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences Warsaw, Poland, Warsaw, Poland

autor

Borowska J.

• College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, Warsaw, Poland

autor

Sadowska M.

• College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, Warsaw, Poland

autor

Jablonka J.

• Laboratory of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Waleszczyk W.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences Warsaw, Poland, Warsaw, Poland