Transcriptomes of Indian barnyard millet and barnyardgrass reveal putative genes involved in drought adaptation and micronutrient accumulation

• Autorzy: Jayakodi M. , Madheswaran M. , Adhimoolam K. , Perumal S. , Manickam D. , Kandasamy T. , Yang T.-J. , Natesan S.
• Języki publikacji: EN

Abstrakty

EN

Indian barnyard millet (Echinochloa frumentacea) is a rich source of dietary fiber, minerals and protein. The lack of genetic resources has slowed the discovery of genes involved in its nutrient accumulation and climate resilience. Here, we present the first transcriptomes of E. frumentacea [97,065 transcripts, including 65,276 protein-coding transcripts, over 90% of which have been functionally annotated, and 31,789 long noncoding RNA (lncRNA) transcripts] and its wild relative E. crus-galli (93,725 transcripts, including 68,480 protein-coding transcripts, 89% of which have been annotated). Comparative transcriptome analysis identified 4159 protein-coding and 2258 lncRNA transcripts in Indian barnyard millet that showed either up- or down-regulated expression when compared with E. crus-galli, and 3489 protein-coding transcripts unique to Indian barnyard millet were also detected. Additional analysis revealed that photosynthesis is likely crucial in the drought adaptation of Indian barnyard millet. We then identified possible genes regulation responsible for drought tolerance and Fe and Zn accumulation. Moreover, based on the simple sequence repeat (SSR)-containing sequence, 30 SSR primer pairs were arbitrarily selected, synthesized and used to screen the 30 E. frumentacea accessions. Of these, 10 SSR primers were polymorphic. Collectively, our results enhance the knowledge of micronutrient accumulation and drought tolerance in Indian barnyard millet, as well as of the genetic diversity of Echinochloa species.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2019
• Tom: 41
• Numer: 05
• Opis fizyczny: Article 66 [11p.], fig.,ref.

Twórcy

autor

Jayakodi M.

• Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151‑921, Republic of Korea

autor

Madheswaran M.

• Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu 625104, India

autor

Adhimoolam K.

• Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu 625104, India

autor

Perumal S.

• Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada

autor

Manickam D.

• Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu 625104, India

autor

Kandasamy T.

• Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu 625104, India

autor

Yang T.-J.

• Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151‑921, Republic of Korea

autor

Natesan S.

• Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu 625104, India
• Centre for Plant Molecular Biology and Biotechnology, Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, India

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Identyfikatory

DOI

10.1007/s11738-019-2855-4