Influence of didecyl dimethyl ammonium bromide on the survival of Salmonella on eggshells

• Autorzy: Radkowski M. , Uradzinski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the research was to determine the influence of selected concentrations of didecyl dimethyl ammonium bromide (DDAB) on the survival rate of Salmonella strains on eggshells. 1,260 chicken eggs on the day they were laid were used. Salmonella enterica serotypes Enteritidis, Tyhimurium, and Agona were inoculated into 9 ml nutrient broth and incubated for 24 h at 37°C. Then 10-fold dilutions of the culture were made and 0.1 ml of each dilution was inoculated on the shell of each egg. Then, each egg was immersed in 200 ml of 0.025%, 0.05%, or 1.0% water solution of DDAB. The eggs were kept for 2 min in the 0.05% and 1% solution and for 2, 5, and 10 min in the 0.025% solution. The results proved that the detection of Salmonella depends on the inoculum of those bacteria on the egg surface. At the contamination rate of 10⁵ cells on the surface of the egg and immersing it for 2 min in 0.025% DDAB solution, there were no Salmonella found. At the contamination rate of 10⁶ CFU of Salmonella and immersing for 5 min in 0.025% DDAB solution, no Salmonella organisms were found. Previous research by the authors has indicated that it is possible to kill Salmonella on the surface of eggs by immersing them in 0.025% DDAB.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.633-636,ref.

Twórcy

autor

Radkowski M.

• Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

autor

Uradzinski J.

• Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

Bibliografia

• 1. Aksu H., Bostan K., Aydin A., Yildirm M., Keles O.: Disinfection of eggshells contaminated with Salmonella Enteritidis. Medycyna Wet 2006, 62, 641-643.
• 2. Anonymous. Salmmonella annual summary 2002. US. Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Bacterial and Mycotic Disease, Foodborne and Diarrhed Diseasea Branch, Atlanta, 2002.
• 3. Announcement of the Minister of Agriculture and Rural Development of 30 June 2000 on a list of pharmaceuticals and medical material used exclusively in animals and approved for trade in Poland. M.P. 2000 No. 26, Item 544, 2000.
• 4. Braden C.R.: Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clin Infect Dis 2006, 43, 512-517.
• 5. Baumann A., Sadkowska-Todys M.: Foodborne infections intoxications in Poland in 2006. Epidemiol Rev 2008, 62, 275-286.
• 6. Catelano C.R., Knabel S.: Destruction of Salmonella Enteritidis by high pH and rapid chilling during simulated commercial egg processing. J Food Prot 1994, 57, 592-595.
• 7. Centers for Disease Control and Prevention. Outbreaks of Salmonella serotype Enteritidis infection associated with eating shell eggs - United States, 1999-2001. Morb Mortal Wkly Rep 2003, 51, 1149-1152.
• 8. Czerwiński M., Czarkowski M.P., Baumann A.: Salmonellosis in Poland in 2006. Epidemiol Rev 2008, 62, 301-310.
• 9. Dominguez A., Torner N., Ruiz L., Martinez A., Bartolome R., Sulleiro E., Teixido A., Plasencia A.: Foodborne Salmonella-caused outbreaks in Catalonia (Spain), 1990 to 2003. J Food Prot 2007, 70, 209-213.
• 10. Duguid J. P., North R. A.E.: Eggs and Salmonella food poisoning: an evaluation. J Med Microbiol 1991, 34, 65-67.
• 11. Himathongkham S., Rieman H., Ernest R.: Efficacy of disinfection of shell eggs externally contaminated with Salmonella Enteritidis. Implications for egg testing. Int J Food Microbiol 1999, 49, 161-167.
• 12. Kist M.J., Freitag S.: Serovar specific risk factors and clinical features of Salmonella enterica sp. serovar Enteritidis: a study in South-West Germany. Epidemiol Infect 2000, 124, 383-392.
• 13. Lievonen S., Havulinna A.S., Maijala R.: Egg consumption patterns and Salmonella risk in Finland. J Food Prot 2004, 67, 2416-2423.
• 14. Little C.L., Surman-Lee M., Greenwood M., Bolton F.J., Elson R., Mitchell R.T., Nichols G.N., Sagoo S.K., Threlfall E.J., Ward L.R., Gillespie I.A., O'-Brien S.O.: Public health investigations of Salmonella Enteritidis in catering raw shell eggs, 2002-2004. Lett Appl Microbiol 2007, 44, 595-601.
• 15. Methner U.: Salmonella control in poultry meat and egg production. Fleischwirtschaft 2001, 81, 85-88.
• 16. Murchie L., Whyte P., Bin Xia., Horigan S., Kelly L., Madden R.H.: Prevalence of Salmonella in grade a whole eggs in the island of Ireland. J Food Prot 2007, 70, 1238- 1240.
• 17. Polish Standard.: PN-ISO 6579. Microbiology - methods for the detection of Salmonella. 1998.
• 18. Poppof M.Y., Le Minor L.: Antigenic formulas of the Salmonella serovars. WHO Collaborating Centre for Reference and Research on Salmonella. Institut Pasteur, Paris, France, 1997.
• 19. Radkowski M.: Occurrence of Salmonella spp. in hen eggs. Medycyna Wet 1990, 46, 331-333.
• 20. Radkowski M.: The effect of some factors on the survival of Salmonella spp. in eggs and in egg powder. Agricult Tech Olst Veterinaria 1994, No. 21.
• 21. Radkowski M.: Occurrence of Salmonella spp. in consumption eggs in Poland. Int J Food Microbiol 2001, 64, 189-191.
• 22. Regulations (EC) No. 2160/2003 of the European Parliament and the Council of 17 November 2003 on the control of Salmonella and other specified food borne zoonotic agents. Off J Europ Union 2003, L 325.
• 23. Regulation of the Minister of Agriculture and Rural Development of 12 March 2004 on the requirements concerning the production of eggs and marketed egg products. O J 2004 No. 52, Item 521.
• 24. Regulation of the Minister of Agriculture and Rural Development of 12 March 2004 on the detailed veterinary requirements concerning the poultry and hatched eggs. O J 2004 No. 219, Item 2225.
• 25. Sheehan B., Oort R. van: Salmonella control: protecting eggs and people. World Poult 2006, 22, 42-44.
• 26. Żórawski C., Skwarek P., Pawiak R.: Microbiological methods used for investigation of chemicals disinfectants applied for veterinary purposes. Edited by the National Veterinary Research Institute, Pulawy, Poland, 1989.