Effect of unilateral striatal 6-hydroksydopamine lesion on ventilatory response to hypoxia in the rat

• Autorzy: Budzinska K. , Andrzejewski K.
• Języki publikacji: EN

Abstrakty

EN

Motor disorders present in patients with Parkinson’s disease (PD) can be mimicked in animals by striatal injection of 6-hydroxydopamine (6-OHDA) that causes a destruction of the dopaminergic nigrostriatal neurons. Since respiratory disturbances accompany PD, the aim of this study was to investigate whether 6-OHDA lesion in the striatum could elicit changes in ventilation and in ventilatory response to hypoxia. Experiments were performed on adult rats that received a microinjection of 6-OHDA or vehicle into the right striatum stereotaxically. Before and 14 days post striatal injection conscious rats were placed in whole body plethysmograph to measure ventilatory parameters as tidal volume, frequency of breathing and minute ventilation during eupnoea and ventilatory response to 3 minutes breathing with 8% hypoxic gas mixture. The hypoxic test was preceded by a behavioral cylinder test to check whether the 6-OHDA lesion was effective in evoking a motor deficit. After the 6-OHDA injection the cylinder test demonstrated a clear preference of the use of the forelimb ipsilateral to the 6-OHDA lesion during rising on the wall of cylinder. The pattern of lung ventilation after the lesion resembled that of the control animals. Breathing with 8% oxygen evoked characteristic hypoxic hyperventilatory response; however in 6-OHDA-lesioned animals mean tidal volume increased more in response to hypoxia than in controls and attained significantly higher amplitude during the first minute of the hypoxic exposure. In conclusion, studies revealed that an increase in chemical drive for respiration due to hypoxia changed the hypoxic ventilatory response in 6-OHDA model of PD. Since dopamine is thought to exert an inhibitory influence on respiratory hypoxic response, an increase of hypoxic hyperventilation following 6-OHDA injection might result from a deficit of dopamine due to degeneration of dopaminergic neurons evoked by 6-OHDA.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.60-61

Twórcy

autor

Budzinska K.

• Department of Respiratory Research, Mossakowski Medical Research Center PAS, Warsaw, Poland

autor

Andrzejewski K.

• Department of Respiratory Research, Mossakowski Medical Research Center PAS, Warsaw, Poland

Uwagi

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