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2009 | 31 | 3 |

Tytuł artykułu

Effect of different media composition on the micropropagation of Erica andevalensis, a metal accumulator species growing in mining areas (SW Spain)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We describe a micropropagation and acclimatization protocol of Erica andevalensis Cabezudo & Rivera, using stem pieces collected from wild plants. This species always grows in soils enriched with metals such as Fe, Pb, Zn and in acid pH (2.5–4). Among the different media and hormone concentration tested, MS medium with ammonium nitrate concentration reduced to a quarter, 2 mg l⁻¹ indole-acetic acid (IAA) and 0.5 mg l⁻¹ kinetin was found to yield the best growth response in the plants. This medium produced an average of 3.6 ± 1.2 shoots and 16.9 ± 8.3 verticils per explant after 30 days of the onset of the culture. MS media achieved rooting without phytohormones. Rooted plantlets were successfully transferred to plastic containers with autoclaved perlite, watered with MS half-strength, and then to pots with commercial substrate after 2 weeks. New plants were produced in about 100 days. As far as we know, this is the first time that a replicable and complete micropropagation protocol for E. andevalensis has been developed. The plants looked healthy with no visual detectable phenotypic variations. The protocol provides a successful technique that could be used for the conservation of the species, as well as new researches on phenolics and triterpenoids compounds found in plant extracts.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

31

Numer

3

Opis fizyczny

p.661-666,fig.,ref.

Twórcy

  • Department of Environmental Biology and Public Health, University of Huelva, Huelva, Spain
  • Avda. Tres de Marzo, S/N, 21071 Huelva, Spain
  • Department of Environmental Biology and Public Health, University of Huelva, Huelva, Spain
autor
  • Department of Environmental Biology and Public Health, University of Huelva, Huelva, Spain

Bibliografia

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  • Aparicio A (1999) Erica andevalensis. In: Blanca G, Cabezudo B, Hernández-Bermejo JE, Herrera CM, Molero-Mesa J, Muñoz J, Valdés B (eds) Libro rojo de la Flora Amenazada de Andalucía. Consejería de Medio Ambiente, Junta de Andalucía, Sevilla, pp 119–122
  • Aparicio A, García-Martín F (1996) The reproductive biology and breeding system of Erica andevalensis Cabezudo & Rivera (Ericaceae), and endangered edaphic endemic of southwestern Spain. Flora 191:345–351
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  • Cabezudo B, Rivera J (1980) Notas taxonómicas y corológicas sobre la flora de Andalucîa Occidental.2.Erica andevalensis Cabezudo & Rivera, SP. NOV. Lagascalia 9:223–226
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  • Heras MA, Hidalgo PJ, Navarro F, Córdoba F (2002) Contenido de metales pesados en Erica andevalensis, un brezo endémico de la Península Ibérica. In: Ramos Castellanos P, Márquez Moreno MC (eds) Avances en calidad ambiental. Ediciones Universidad Salamanca, pp 329–334
  • IUCN Red List Categories and Criteria (2001) version 3.1. IUCN Species Survival. Commission, IUCN, Gland, Switzerland and Cambridge, UK
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  • Martín-Cordero C, Reyes M, Ayuso MJ, Toro MV (2001) Cytotoxic triterpenoids from Erica andevalensis. Z Naturforsch [C] 56:45–48
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  • Nelson EC, McClintock D, Small D (1985) The natural habitat of Erica andevalensis in south-werstern Spain. Kew Mag 2:324–330
  • Reyes Ruiz M, Martín Cordero C, Ayuso González MJ, Toro Sainz MV, Alarcón de la Lastra C (1996) Antiulcer activity in rats by flavonoids of Erica andevalensis Cabezudo-Rivera. Phytother Res 10:300–303. doi:10.1002/(SICI)1099-1573(199606)10:4<300::AID-PTR844>3.0.CO;2-Z
  • Rodríguez N, Amils R, Jiménez-Ballesta R, Rufo L, Fuente V (2007) Heavy metal content in Erica andevalensis: an endemic plant from the extreme acidic environment of Tinto River and its soils. Arid Land Res Manage 21:51–67. doi:10.1080/15324980601074578
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  • Viémont JD, Beaujard F (1983) Les bruyères in vitro. II. Elongation et organogenèse lors de la croissance rythmique che Erica x darleyensis. Bull Soc Bot Fr 130:317–324
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Typ dokumentu

Bibliografia

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