SRC kinase mRNA transcription changes in testosterone-induced rat ventral prostate lobes under the influence of Epilobium angustifolium extract

• Autorzy: Kujawski R. , Bartkowiak-Wieczorek J. , Bogacz A. , Karasiewicz M. , Mikolajczak P. L. , Czerny B. , Mrozikiewicz P. M.

Warianty tytułu

PL

Zmiany liczby transkryptów kinazy SRC w brzusznym płacie prostaty szczurów indukowanych hormonalnie pod wpływem wyciągu z ziela Epilobium angustifolium

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the influence of standardized crude aqueous Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of SRC kinase mRNA - a representatives of non-genomics xenobiotics signaling pathway in prostate ventral lobes of testosterone-induced, castrated rats. We have shown that in all analyzed groups induced by testosterone an elevation of SRC kinase mRNA transcription was observed, in comparison to control animals (not receiving the testosterone), (p<0.05). Finasteride in rats induced by testosterone caused the strongest inhibition of SRC mRNA transcription (p<0.05). In rats receiving testosterone and the plant extract a ca. 90% decrease of mRNA level was observed vs. testosterone-induced animals (p<0.05), while in testosterone-induced animals receiving concomitantly E. angustifolium extract and finasteride the observed reduction reached 87.3% (p<0.05). We did not observed, however, any positive feedback between studied plant extract and finasteride in the inhibitory activity (p<0.05). Further experimental studies should be performed in order to the understanding the molecular basis of interactions, the efficacy and safety of tested plant extract.

PL

Celem pracy było zbadanie wpływu standaryzowanego ekstraktu z ziela Epilobium angustifolium L. [100 mg/kg/dzień, p.o.] na poziom transkrypcji mRNA kinazy SRC - przedstawiciela androgenozależnego, niegenomowego szlaku sygnalizacji komórkowej w brzusznym płacie prostat indukowanych hormonalnie, kastrowanych szczurów. We wszystkich grupach szczurów indukowanych testosteronem zaobserowaliśmy wzrost liczby transkryptów kinazy SRC, w porównaniu do zwierząt kontrolnych (p<0.05). U szczurów indukowanych hormonalnie finasteryd wykazał najsilniejsze właściwości hamujące transkrypcję mRNA (p<0.05). U zwierząt otrzymujących testosteron wraz z wyciągiem z E. angutifolium stwierdziliśmy obniżenie liczby badanych transkryptów o blisko 90%, w porownaniu ze zwierzętami z grupy kontrolnej, indukowanymi testosteronem (p<0.05). U szczurów otrzymujących jednocześnie testosteron i ekstrakt roślinny łącznie z finasterydem redukcja poziomu mRNA kinazy SRC sięgała 87.3% (p<0.05). Nie zaobserwowaliśmy dodatniego sprzężenia w inhibicji transkrypcji badanego mRNA u szczurów otrzymujących badany ekstrakt wraz z finasterydem (p<0.05). Istnieje potrzeba przeprowadzenia dalszych badań zmierzających do wyjaśnienia molekularnego podłoża interakcji, mechanizmu działania oraz skuteczności badanego wciągu roślinnego.

Słowa kluczowe

EN

Src kinase; mRNA; transcription change; testosterone; rat; ventral prostate lobe; prostate; Epilobium angustifolium; plant extract

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2013
• Tom: 59
• Numer: 4
• Opis fizyczny: p.60-71,fig.,ref.

Twórcy

autor

Kujawski R.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Bartkowiak-Wieczorek J.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Bogacz A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Karasiewicz M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Mikolajczak P. L.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Chair and Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland

autor

Czerny B.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Department of General Pharmacology and Pharmacoeconomy, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland

autor

Mrozikiewicz P. M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

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Identyfikatory

DOI

10.2478/hepo-2013-0024