Year-round blueberry scorch virus detection in highbush blueberry

• Autorzy: Paduch-Cichal E. , Chodorska M. , Kalinowska E. , Komorowska B.

Warianty tytułu

PL

Wykrywanie wirusa oparzeliny borówki wysokiej w różnych okresach roku

• Języki publikacji: EN

Abstrakty

EN

Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].

PL

Celem przeprowadzonych badań było wykrywanie i identyfikacja wirusa oparzeliny borówki wysokiej (Blueberry scorch virus, BlScV) w różnych organach pobieranych z krzewów borówki wysokiej odmian Bluecrop i Herbert rosnących na plantacji produkcyjnej zlokalizowanej w centralnej Polsce. Badania byáy prowadzone w okresie od maja 2010 do kwietnia 2011 r. przy użyciu testu serologicznego DAS-ELISA. Próby materiału roślinnego (pąki kwiatowe, kwiaty, liście, kora) pobierano indywidualnie z krzewów kaĪdej z badanych odmian. Ustalono, że wykrywanie wirusów w krzewach odmian Bluecrop i Herbert zależało od testowanego organu oraz terminu, w którym przeprowadzono test. Obecność BlScV w krzewach badanych odmian potwierdzono przy pomocy techniki RT-PCR z wykorzystaniem starterów amplifikujących fragment 5’ genu kodującego polimerazą RNA zależną od RNA.

Słowa kluczowe

EN

plant cultivar; blueberry; blueberry scorch virus; scorch disease; plant disease; detection; Northern highbush blueberry; viral disease

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 3
• Opis fizyczny: p.3-11,fig.,ref.

Twórcy

autor

Paduch-Cichal E.

• Department of Plant Pathology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Chodorska M.

• Department of Plant Pathology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Kalinowska E.

• Department of Plant Pathology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Komorowska B.

• Research Institute of Horticulture, Skierniewice, Poland

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