Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 days of cold storage

• Autorzy: Iwanowska A. , Iwanska E. , Grzes B. , Mikolajczak B. , Pospiech E. , Rosochacki S. , Juszczuk-Kubiak E. , Lyczynski A.

Warianty tytułu

PL

Zmiany zachodzące w białkach i kruchości młodej wołowiny zależnie od rasy i wieku ubijanych zwierząt

• Języki publikacji: EN

Abstrakty

EN

The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar part of the longissimus dorsi (LD) muscle of Polish Holstein-Friesian (PHF) Black-and-White variety, Polish Red (PR), Hereford (H) and Limousine (L) bulls slaughtered at the age of 6, 9 and 12 months. Muscle analyses were carried out 45 min and 48, 96 and 240 h postslaughter, separating proteins with the assistance of SDS-PAGE (electrophoresis in polyacrylamide gel using SDS). To identify titin, desmin and troponin T (Tn-T), their antibodies and western blotting were employed. The breed occurred to be the key factor affecting proteins’ changes in the muszle tissue. The process of protein degradation in PHF was similar to that found in H while in PR – to that occurring in L bulls, despite the genotype differences between them. The greatest differences in protein changes were found between the meat obtained from bulls at the age of 6 vs. 12 months. During meat cold storage, day 10 turned out to be critical with regard to the degradation of almost all proteins. The drop of the content of titin, desmin and Tn-T was observed, simultaneously with the increase in their degradation products. The highest myofibrillar protein degradation observed on day 10 of cold storage proves these changes.

PL

Analizowano zmiany następujące w białkach i kruchości młodej wołowiny przechowywanej w chłodni, zależnie od genotypu i wieku ubitych zwierząt. Badania objęły część piersiową i lędźwiową mięśnia najdłuższego grzbietu (LD) byczków czterech ras – holsztyno-fryzyjskiej odmiany czarnobiałej (PHF), polskiej czerwonej (PR), hereford (H) i limousine (L), ubijanych w wieku 6, 9 i 12 miesięcy. Identyfikacji białek dokonano w 45 min i dalej w 48, 96 i 240 godzinie po uboju, za pomocą elektroforezy w żelu poliakrylamidowym z wykorzystaniem SDS (SDS-PAGE). W identyfikacji titiny,desminy i troponiny T (Tn-T) zastosowano przeciwciała tych białek i immunoblotting. Stwierdzono, że głównym czynnikiem wpływającym na udział badanych białek w tkance mięśniowej była rasa (a zatem genotyp) buhajków. Zaobserwowano podobieństwa w procesie degradacji białek między buhajkami PHF a H oraz między PR a L, mimo że reprezentują one różne genotypy. Uwzględniając oddziaływanie wieku,największe różnice w przemianach białek stwierdzono między mięsem uzyskanym od byczków 6- a 12-miesięcznych. Podczas składowania chłodniczego dzień 10 okazał się krytyczny pod względem degradacji większości białek. Zmniejszył się udział titiny, desminy i Tn-T, natomiast wzrósł udział produktów ich degradacji. Potwierdzeniem większych zmian degradacyjnych białek miofibryli była najlepsza kruchość mięsa w 10 dniu przechowywania.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2010
• Tom: 28
• Numer: 1
• Opis fizyczny: p.13-25,fig.,ref.

Twórcy

autor

Iwanowska A.

• Institute of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland

autor

Iwanska E.

• Institute of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland

autor

Grzes B.

• Institute of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland

autor

Mikolajczak B.

• Institute of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland

autor

Pospiech E.

• Institute of Meat Technology, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland
• Meat and Fat Research Institute in Poznan, Głogowska 239, 60-111 Poznan, Poland

autor

Rosochacki S.

• Institute of Genetics and Animal Breeding, Jastrzebiec, 05-552 Wolka Kosowska, Poland
• Chair of Sanitary Biology and Biotechnology, Bialystok Technical University, Wiejska 45e, 15-351 Bialystok, Poland

autor

Juszczuk-Kubiak E.

• Institute of Genetics and Animal Breeding, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Lyczynski A.

• Department of Animal Origin Materials, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland

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