Changes in blood cytokine concentrations in horses after long-distance endurance rides

• Autorzy: Cywinska A. , Turlo A. , Witkowski L. , Szarska E. , Winnicka A.
• Języki publikacji: EN

Abstrakty

EN

Long-distance endurance rides involve strenuous effort, which induces numerous changes in the horse’s body, including the exercise-induced acute-phase response (APR). Such a reaction has also been reported in humans and dogs after exertion, but it varies depending on the species and the type of exercise. In horses, the exerciseinduced APR is manifested as an increase in serum amyloid A (SAA) after the effort, but the mechanism and regulation of this process has not been clearly understood. The aim of this study was therefore to determine the changes in the concentration of cytokines that are believed to regulate this type of reaction. Twelve horses competing in 120 km and 160 km endurance rides were included in the study. The routine haematological and biochemical blood test, as well as the measurements of SAA, IL-1, IL-4, IL-6, IL-10, and TNFα, were carried out before and after competitions. Typical haematological changes and increases in SAA levels were accompanied by increases in IL-6 and IL-10 concentrations, which were also positively correlated before the effort. Taking into account the sampling time, it is postulated that the exercise-induced APR is promoted by type 1 cytokines. It has also been concluded that the exercise-induced APR in horses in regular training is accompanied by strong a anti-inflammatory response, which prevents clinical disorders after long-distance rides. Then, the overall “anti-inflammatory state” defined previously in race horses is also likely to occur in endurance horses.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 09
• Opis fizyczny: p.568-571,fig.,ref.

Twórcy

autor

Cywinska A.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Turlo A.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Witkowski L.

• Laboratory of Veterinary Epidemiology and Economic Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Szarska E.

• Military Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Winnicka A.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

Bibliografia

• 1. Cappelli K., Barrey E., Capomaccio S., Felicetti M., Silvestrelli M., Supplizi A. V.: Exercise influence on gene-expression profile in endurance horse PBMC. Ital. J. Anim. Sci. 2009, 8 (Suppl. 2), 730.
• 2. Cywinska A., Gorecka R., Szarska E., Witkowski L., Dziekan P., Schollenberger A.: Serum amyloid A as a potential indicator of the status of endurance horses. Equine Vet. J. 2010, 42, (Suppl 38), 23-27.
• 3. Cywińska A., Szarska E., Gorecka R., Witkowski L., Hecold M., Berezowski A., Schollenberger A., Winnicka A.: Acute phase protein concentrations after limited distance and long distance endurance rides in horses. Res. Vet. Sci. 2012, 93, 1402-1406.
• 4. Cywinska A., Witkowski L., Szarska E., Schollenberger A., Winnicka A.: Serum amyloid A (SAA) concentration after training sessions in Arabian race and endurance horses. BMC Vet. Res. 2013, 9, 91. doi: 10.1186/1746-6148-9-91.
• 5. Eckersall P. D.: Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Vet. Med. Rev. 2000, 151, 577-584.
• 6. Fallon K. E.: The acute phase response and exercise: the ultramarathon as prototype exercise. Clin. J. Sport Med. 2001, 11, 38-43.
• 7. Gruys E., Toussaint M. J. M., Niewold T. A., Koopmans S. J.: Acute phase reaction and acute phase proteins. Journal of Zhejiang University SCIENCE 2005, 11, 1045-1056.
• 8. Helge J. W., Stallknecht B., Pedersen B. K., Galbo H., Kiens B., Richter E. A.: The effect of graded exercise on IL-6 release and glucose uptake in human skeletal muscle. J. Physiol. 2003, 546, 299-305.
• 9. Hinchcliff H., Kaneps A., Geor R.: Equine Sports Medicine and Surgery. Saunders 2004, 937-1013.
• 10. Horohov D. W., Sinatra S. T., Chopra R. K., Jankowitz S., Betancourt A., Bloomer R. J.: The effect of exercise and nutritional supplementation on proinflammatory cytokine expression in young racehorses during training. J. Equine Vet. Sci. 2012, 32, 805-815.
• 11. Khan F. A., Khan M. F.: Inflammation and acute phase response. Int. J. App. Biol. Pharm. Technol. 2010, 1, 313-321.
• 12. Kilicarslan A., Uysal A., Roach E. C.: Acute Phase Reactants. Acta Med. 2013, 2, 2-7.
• 13. Liburt N. R., Adams A. A., Betancourt A., Horohov D. W., McKeever K. H.: Exercise-induced increases in inflammatory cytokines in muscle and blood of horses. Equine Vet. J. 2010, 42, (Suppl 38), 280-288.
• 14. Niedźwiedź A., Kubiak K., Nicpoń J.: Plasma total antioxidant status in horses after 8-hours of road transportation. Acta Vet. Scand. 2013, 55, doi: 10.1186/1751-0147-55-58.
• 15. Niedźwiedź A., Zawadzki M., Filipowski H., Nicpoń J.: Influence of 8-hour road transportation on selected physiological parameters in horses. Bull. Vet. Inst. Pulawy 2012, 56, 193-197.
• 16. Peeling P., Dawson B., Goodman C., Landers G., Trinder D.: Athletic induced iron deficiency: new insights into the role of inflammation, cytokines and hormones. Eur. J. App. Physiol. 2008, 103, 381-391.
• 17. Pedersen B. K.: The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays Biochem. 2006, 42, 105-117.
• 18. Petersen H. H., Nielsen J. P., Heegaard P. M. H.: Application of acute phase protein measurements in veterinary clinical chemistry. Vet. Res. 2004, 35, 163-187.
• 19. Scharhag J., Meyer T., Gabriel H. H. W., Schlick B., Faude O., Kindermann W.: Does prolonged cycling of moderate intensity affect immune cell function? Br. J. Sports Med. 2005, 39, 171-177.
• 20. Steensberg A., Toft A. D., Schjerling P., Halkjaer-Kristensen J., Pedersen B. K.: Plasma interleukin-6 during strenuous exercise: role of epinephrine. Am. J. Physiol. Cell Physiol. 2001, 281, C1001-1004.
• 21. Streltsova J. M., McKeever K. H., Liburt N. R., Gordon M. E., Filho H. M., Horohov D. W., Rosen R., Franke W.: Effect of orange peel and black tea extracts on markers of performance and cytokine markers of inflammation in horses. Equine Comp. Exerc. Physiol. 2006, 3, 121-130.
• 22. Suzuki K., Peake J., Nosaka K., Okutsu M., Abbiss C. R., Surriano R., Bishop D., Quod M. J., Lee H., Martin D. T., Laursen P. B.: Changes in markers of muscle damage, inflammation and HSP70 after an Ironman triathlon race. Eur. J. App. Physiol. 2006, 98, 525-534.
• 23. Szarska E.: Investigations of blood parameters for evaluating of health status and training effects in race and sport horses. Zeszyty Naukowe AR, Wroclaw 2003, 471, 1-115.
• 24. Tizard I. R.: Veterinary immunology, an introduction. 8th ed., Saunders Elsevier 2009.
• 25. Wakshlag J. J., Kraus M. S., Gelzer A. R., Downey R. L., Vacchani P.: The influence of high-intensity moderate duration exercise on cardiac troponin I and C-reactive protein in sled dogs. J. Vet. Int. Med. 2010, 24, 1388-1392.
• 26. Wakshlag J. J., Stokol T., Geske S. M., Greger C. E., Angle C. T., Gillette R. L.: Evaluation of exercise-induced changes in concentrations of C-reactive protein and serum biochemical values in sled dogs competing in a long-distance endurance race. Am. J. Vet. Res. 2010, 71, 1207-1213.
• 27. Woods J. A., Vieira V. J., Keylock K. T.: Exercise, inflammation, and innate immunity. Immunol. Allergy Clin. North Am. 2009, 29, 381-393.

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