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2014 | 63 | 3 |

Tytuł artykułu

Could a step in the isolation of mycobacteria from sputum samples be eliminated by new decontamination kits?

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The efficacy of two mycobacterial homogenization, decontamination and concentration kits, Mycoprosafe and Decomics, were compared. A total of 146 sputum samples were examined in this study; 46 (31.5%) mycobacterial isolates were recovered with Mycoprosafe, while 39 (26.7%) mycobacterial isolates were recovered with Decomics. These results, although very preliminary, indicate that Mycoprosafe and Decomics are similar in terms of ease of use and price. However, Decomics offers several advantages, including the need for less equipment, a more rapid detection time, and an easy shipping process; thus, pre-processing can be done anywhere.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

63

Numer

3

Opis fizyczny

p.369-371,ref.

Twórcy

autor
  • Department of Medical Microbiology, School of Medicine, Sakarya University, Sakarya, Turkey
autor
  • Department of Medical Microbiology, School of Medicine, Sakarya University, Sakarya, Turkey
autor
  • Department of Medical Microbiology, School of Medicine, Sakarya University, Sakarya, Turkey

Bibliografia

  • Ganoza C.A., J.N. Ricaldi, J. Chauca, G. Rojas, C. Munayco, J. Agapito, J.C. Palomino and H. Guerra. 2008. Novel hypertonic saline-sodium hydroxide (HS-SH) method for decontamination and concentration of sputum samples for Mycobacterium tuberculosis microscopy and culture. J. Med. Microbiol. 57: 1094–1098.
  • Kubica G.P., W.E. Dye, M.L. Cohn and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 89: 284–286.
  • Kurtoğlu M.G., M. Ozdemir, R. Keşli, B. Ozkalp and B. Baysal. 2011. Tüberküloz Şüpheli Hastalardan Mycobacterium tuberculosis Kompleks İzolasyon Oranı ve Suşların BACTECTM NAP ve İmmünokromatografik TB Ag MPT64 RapidTMTestleri ile Tanımlanması. Mikrobiyol. Bul. 45(2): 266–273.
  • Maciel E.L., T.N. Prado, R.L. Peres, M. Palaci, J.L. Johnson and R. Dietze. 2009. Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary. TB. J. Bras. Pneumol. 35(5): 460–463.
  • Martin M.E., C.L. Horton, A.L. Sheffner and J.D. Solonon. 1968. Direct comparison of the N-acetyl-L-cysteine-sodium hydroxide and the trisodium phosphate digestion methods for the culture of mycobacteria. Appl. Microbiol. 16: 506–508.
  • Peres R.L., E.L. Maciel, C.G. Morais, F.C.K. Ribeiro, S.A. Vinhas, C. Pinheiro, R. Dietze, J.L. Johnson, K. Eisenach and M. Palaci. 2009. Comparison of two concentrations of NALC-NaOH for decontamination of sputum for mycobacterial culture. Int. J. Tuberc. Lung. Dis. 13(12): 1572–1575.
  • Ratnam S., F.A. Stead and M. Howes. 1987. Simplified acetylcysteine-alkali digestion-decontamination procedure for isolation of mycobacteria from clinical specimens. Clin. Microbiol. 25(8): 1428–1432.
  • Rivas C., C. Coitinho, V. Dafond, M. Corbo and M. Baldjian. 2010. Performance of the Ogawa-Kudoh method for isolation of mycobacteria in a laboratory with large-scale workload. Rev. Argent. Microbiol. 42(2): 87–90.
  • Yassin M.A. and L.E. Cuevas. 2003. How many sputum smears are necessary for case finding in pulmonary tuberculosis? Tropical Medicine and International Health 8 (10): 927–932.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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