Analysis of ovary DNA methylation during delayed fertilization in hazel using the methylation-sensitive amplification technique

• Autorzy: Cheng Y. , Wang J. , Liu J. , Zhao Y. , Geng W. , Zhang H.
• Języki publikacji: EN

Abstrakty

EN

Hazel (Corylus avellana L.) produces edible nuts of great economic value and has unique delayed fertilization characteristics. The ovary in hazel starts to develop only after pollination, and a growth signal from pollen tubes is thought to be the key factor that triggers this process. DNA methylation may play an important role in the epigenetic activation of ovary development in hazel; however, changes in DNA methylation during the delayed fertilization process remain unknown. In this study, we assessed DNA methylation changes during pre- and postpollination stages using the methylation-sensitive amplification polymorphism (MSAP) technique. A total of 760 50- CCGG-30 amplified sites were detected by MSAP assay. The total methylation ratio of hazel was relatively high, ranging from 44.61 to 48.68 %, while after pollination, it decreased slightly by approximately 4 %. MSAP bands were significantly different between pre- and post-pollination stages, and a total of 15 differentially amplified bands (L01–L15) were cloned for sequence analysis. Most of the fragments were homologous to gene exons or mitochondrial sequences. Quantitative reverse transcription polymerase chain reaction showed that the gene-body methylation of three differentially amplified bands (L08, L10, and L13) was closely associated with their gene expression level. Bisulfite sequencing of L08 showed that cytosine methylation and demethylation changes varied in different pre- and post-pollination stages. In conclusion, epigenetic changes probably represent an important mechanism that initiates ovary and ovule development after pollination in hazel in which preferential gene-body methylation is a key feature.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 231 [12 p.], fig.,ref.

Twórcy

autor

Cheng Y.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

autor

Wang J.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

autor

Liu J.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

autor

Zhao Y.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

autor

Geng W.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

autor

Zhang H.

• College of Life Sciences, Jilin Normal University, Siping, 136000, Jilin Province, China

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Identyfikatory

DOI

10.1007/s11738-015-1984-7