A comparison of the efficiency of molecular and conventional methods in the diagnosis of cutaneous leishmaniosis

• Autorzy: Khoshnood S. , Mohaghegh M.A. , Ghafori K. , Taheri M. , Tavalla M. , Ghorbani M. , Hejazi S.H.
• Języki publikacji: EN

Abstrakty

EN

Leishmaniosis is one of the most important vector borne diseases. Among different forms of the disease, cutaneous leishmaniosis (CL) is the most common. Determining the method of definitive diagnosis for the disease has been the aim of various studies. Therefore this study afforded an opportunity to investigate this subject. To diagnose CL in 150 suspected patients referred to Mehran and Dehloran health centers during June 2018 to November 2019, two polymerase chain reaction (PCR) methods were performed and compared with the in vitro culture and microscopic evaluation of stained slides. The smears were stained with Giemsa for microscopy and cultured in Novy-Nicolle-McNeal (NNN) blood agar for promastigote growth. For semi-nested PCR and PCR-RFLP, the tissue and serosity from the lesions were used for DNA extraction. The semi-nested PCR technique using minicircle kDNA gene showed the highest positivity rates among all diagnostic assays with 114/150 (76%) of the samples and was used as reference standard, followed by the PCR-RFLP test using ITS1 gene with 112/150, (74.7%) positivity rates, microscopy with 101/150 (67.3%) and then culture 72/150 (48%). microscopy and culture methods together improved overall positivity rates to 68.7% (103/150). The all positive samples using molecular technique were identified as Leishmania major. The highest sensitivity (98.3%), specificity (100%), accuracy (98.8%), negative predictive value (94.7%) and κ coefficient (0.96=almost perfect) was observed by comparing PCR-RFLP and semi-nested PCR. kDNA-semi-nested PCR and ITS1-PCR-RFLP presented an interesting alternative to conventional methods for the identification of CL and improved its diagnostic value significantly in suspected patients with negative direct smears.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2021
• Tom: 67
• Numer: 1
• Opis fizyczny: p.39-44,fig.,ref.

Twórcy

autor

Khoshnood S.

• Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences; Hezar Jerib Street, Isfahan 81746-73461, Isfahan, Iran

autor

Mohaghegh M.A.

• Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences; Razi Street, Torbat Heydariyeh 33787-95196, Iran
• Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences; Razi Street, Torbat Heydariyeh 33787-95196, Iran

autor

Ghafori K.

• Department of Geography, Faculty of Humanities, University of Zanjan; University Blvd., Zanjan 45371-38791, Iran

autor

Taheri M.

• Healthcare Center Laboratory, Ahvaz Jundishapur University of Medical Sciences; University Square, Golestan Blvd., Ahvaz 15794-61357, Iran

autor

Tavalla M.

• Department of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences; University Square, Golestan Blvd., Ahvaz 15794-61357, Iran

autor

Ghorbani M.

• Department of Public Health, School of Health, Torbat Heydariyeh University of Medical Sciences; Razi Street, Torbat Heydariyeh 33787-95196, Iran
• Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences; Razi Street, Torbat Heydariyeh 33787-95196, Iran

autor

Hejazi S.H.

• Department of Medical Parasitology and Mycology, School of Medicine, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences; Hezar Jerib Street, Isfahan 81746-73461, Isfahan, Iran

Identyfikatory

DOI

10.17420/ap6701.310