In vitro slow growth storage of Senecio macrophyllus shoots

• Autorzy: Trejgell A. , Kaminska M. , Tretyn A.
• Języki publikacji: EN

Abstrakty

EN

Advances in biotechnology, especially in the field of in vitro culture techniques, led to the development of procedures that can be used as an excellent tool in plant conservation. The present study describes in vitro conservation of Senecio macrophyllus by slow-growth storage. Various sugar treatments, concentration of abscisic acid (ABA), light intensity and type of containers were tested Viability and proliferation rate of shoots were evaluated 4 weeks after regrowth. The results obtained showed that polycarbonate boxes were a better type of containers for storage of S. macrophyllus shoots than glass vessels. Light negatively affected culture viability, multiplication rate and rooting response. In the case of Senecio macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on medium without ABA. Shoots of S. macrophyllus were effectively stored for 6 months. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 234 [9 p.], fig.,ref.

Twórcy

autor

Trejgell A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland

autor

Kaminska M.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland

autor

Tretyn A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland

Bibliografia

• Bairu MW, Stirk WA, Van Staden J (2009) Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tiss Org Cult 98:239–248
• Bonnier FJM, Van Tuyl JM (1997) Long term in vitro storage of lily: effects of temperature and concentration of nutrients and sucrose. Plant Cell Tiss Org Cult 49:81–87
• Burritt DJ (2008) Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation-dehydration requires pretreatment with both ABA and proline. Plant Cell Tiss Org Cult 95:209–215
• Capuana M, Di Lonardo S (2013) In vitro conservation of chestnut (Castanea sativa) by slow growth. In Vitro Cell Dev Biol-Plant 49:605–610
• Dörffling K, Dörffling H, Luck E (2009) Improved frost tolerance and winter hardiness in proline overaccumulating winter wheat mutants obtained by in vitro-selection is associated with increased carbohydrate, soluble protein and abscisic acid (ABA) levels. Euphytica 165:545–556
• Gollagunta V, Adelberg JW, Rieck J, Rajapakse N (2005) Sucrose in storage media and cultivar affects post-storage regrowth of in vitro Hosta propagules. Plant Cell Tiss Org Cult 80:191–199
• Grout BWW (1995) Low temperature storage of plant tissue cultures. In: Aiken-Christie J et al (eds) Automtion and environmental control in plant tissue culture. Kluwer, Dordrecht, pp 517–538
• Hausman JF, Neys O, Kevers C, Gaspar T (1994) Effect of in vitro storage at 4ºC on survival and proliferation of poplar shoots. Plant Cell Tiss Org Cult 38:65–67
• Kobayashi F, Takumi S, Nakamura C (2008) Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat. J Plant Physiol 165:224–232
• Koçİ, Akdemir H, Onay A, Çiftçi YÖ (2014) Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets. Acta Physiol Plant 36:2373–2384
• Kovalchuk I, Lyudvikova Y, Volgina M, Reed BM (2009) Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell Tiss Organ Cult 96:127–136
• Lambardi M, Roncasaglia R, Previati A, De Carlo A, Dradi G, Da Re F, Calamai L (2006) In vitro slow growth storage of fruit rootstock inside gas-tight or gas-permeable containers. Acta Hort 725:483–488
• Lata H, Moraes RM, Bertoni B, Pereira AMS (2010) In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In Vitro Cell Dev Biol-Plant 46:22–27
• Maryam A, Tariq R, Chuadhary S, Azmat R, Javed S, Khanam S (2014) A review: role of tissue culture (in vitro) techniques in the conservation of rare and endangered species. Pac J life Sci 2:93–103
• Moges AD, Kara NS, Shibli RS (2003) Slow growth in vitro preservation of African Violet (Saintpaulia ionantha Wendl.). Adv Hort Sci 17:223–230
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:437–497
• Orlikowska T (1992) Effect of in vitro storage at 4ºC on survival and proliferation of two apple rootstocks. Plant Cell Tiss Org Cult 31:1–7
• Pruski K, Kozai T, Lewis T, Astatkie T, Nowak J (2000) Sucrose and light effect on in vitro cultures of potato, chokecherry and saskatoon berry during low temperature storage. Plant Cell Tiss Org Cult 63:215–221
• Rao NK (2004) Plant genetic resources: advancing conservation and use through biotechnology. Afr J Biotechnol 3:136–145
• Reed BM (1992) Cold storage of strawberries in vitro: a comparison of three storage system. Fruit Var J 46:98–102
• Reed BM (2002) Photoperiod improves long-term survival of in vitrostored strawberry plantlets. HortScience 37:811–814
• Reed BM, Sarasan V, Kane M, Bunn E, Pence VC (2011) Biodiversity conservation and conservation biotechnology tools. In Vitro Cell Dev Biol Plant 47:1–4
• Renau-Morata B, Arrillaga I, Segura J (2006) In vitro storage of cedar shoot cultures under minimal growth conditions. Plant Cell Rep 25:636–642
• Romano A, Martins-Loução MA (1999) In vitro cold storage of cork oak shoot cultures. Plant Cell Tiss Org Cult 59:155–157
• Rybczyński JJ, Mikuła A (2006) Engagement of biotechnology in the protection of threatened plant species in Poland. Biodiv Res Conserv 3–4:361–368
• Sarasan V (2010) Importance of in vitro technology to future conservation programmes worldwide. Kew Bull 65:549–554
• Sarkar D, Naik PS (1998) Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica 102:275–280
• Shibli RS, Shatnawi MA, Subaih WS, Ajlouni MM (2006) In vitro conservation and cryopreservation of plant genetic resources: a review. World J Agric Sci 2:372–382
• Trejgell A, Michalska M, Tretyn A (2010) Micropropagation of Senecio macrophyllus M. Bieb. Acta Biol Crac 52:67–74
• Wilen RW, Gusta LV, Lei B, Abrams SR, Ewan BE (1994) Affects of abscisic acid (ABA) and ABA analogs on freezing tolerance, low-temperature growth, and flowering in rapeseed. J Plant Growth Regul 13:235–241
• Withers LA, Engelmann (1997) In vitro conservation of plant genetic resources. In: Altman A (ed) Biotechnology in agriculture. Marcel Dekker, New York, pp 57–88
• Zarzycki K, Szela˛g Z (2006) Red list of the vascular plants in Poland. In: Mirek Z (ed) Red list of plants and fungi in Poland. W. Szafer Institute of Botany Polish Academy of Sciences, Kraków, pp 9–20

Identyfikatory

DOI

10.1007/s11738-015-1983-8