Campylobacter fetus is internalized by bovine endometrial epithelial cells

• Autorzy: Campos-Muzquiz L.G. , Mendez-Olvera E.T. , Arellano-Reynoso B. , Martinez-Gomez D.
• Języki publikacji: EN

Abstrakty

EN

Campylobacter fetus is an important venereal pathogen of cattle that causes infertility and abortions. It is transmitted during mating, and it travels from the vagina to the uterus; therefore, an important cell type that interacts with C. fetus are endometrial epithelial cells. Several virulence factors have been identified in the genome of C. fetus, such as adhesins, secretion systems, and antiphagocytic layers, but their expression is unknown. The ability of C. fetus to invade human epithelial cells has been demonstrated, but the ability of this microorganism to infect bovine endometrial epithelial cells has not been demonstrated. Bovine endometrial epithelial cells were isolated and challenged with C.fetus. The presence of C. fetus inside the endometrial epithelial cells was confirmed by the confocal immunofluorescence. C. fetuswas not internalized when actin polymerization was disturbed, suggesting cytoskeleton participation in an internalization mechanism. To evaluate the intracellular survival of C. fetus, a gentamicin protection assay was performed. Although C. fetus was able to invade epithelial cells, the results showed that it did not have the capacity to survive in the intracellular environment. This study reports for the first time, the ability of C. fetus to invade bovine endometrial epithelial cells, and actin participation in this phenomenon.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2019
• Tom: 68
• Numer: 2
• Opis fizyczny: p.217-224,fig.,ref.

Twórcy

autor

Campos-Muzquiz L.G.

• Doctorado en Ciencias Agropecuarias, Universidad Autonoma Metropolitana, Xochimilco, Mexico

autor

Mendez-Olvera E.T.

• Doctorado en Ciencias Agropecuarias, Universidad Autonoma Metropolitana, Xochimilco, Mexico

autor

Arellano-Reynoso B.

• Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Ciudad de Mexico, Mexico

autor

Martinez-Gomez D.

• Doctorado en Ciencias Agropecuarias, Universidad Autonoma Metropolitana, Xochimilco, Mexico

Bibliografia

• Ali A, Soares SC, Santos AR, Guimarães LC, Barbosa E, Almeida SS, Abreu VAC, Carneiro AR, Ramos RTJ, Bakhtiar SM, etal.Campylobacter fetus subspecies: comparative genomics andprediction of potential virulence targets. Gene. 2012 Oct;508(2): 145–156. doi:10.1016/j.gene.2012.07.070 Medline
• Bacon DJ, Szymanski CM, Burr DH, Silver RP, Alm RA, Guerry P. A phasevariable capsule is involved in virulence of Campylobacter jejuni 81-176. Mol Microbiol. 2001 May;40(3):769–777. doi:10.1046/j.1365-2958.2001.02431.x Medline
• Baker NT, Graham LL. Campylobacter fetus translocation across Caco-2 cell monolayers. Microb Pathog. 2010 Nov;49(5):260–272. doi:10.1016/j.micpath.2010.06.008 Medline
• Chiapparrone ML, Morán PE, Echevarría HM, Soto P, Paolic-chi FA, Catena M.Campylobacter fetus subsp. venerealis adhesion to MDBK cells. Rev Argent Microbiol. 2014;46(3):269–270. doi:10.1016/S0325-7541(14)70081-1 Medline
• Chiapparrone ML, Soto P, Catena M. Characterization of the Campylobacter fetus subsp. venerealis adhesion to bovine sperm cells. Int. J. Morphol. 2016;34(4):1419–1423. doi:10.4067/S0717-95022016000400040
• Duell BL, Cripps AW, Schembri MA, Ulett GC. Epithelial cell coculture models for studying infectious diseases: Benefits and limitations. J Biomed Biotechnol. 2011;2011:852419. doi:10.1155/2011/852419 Medline
• Eisenreich W, Heesemann J, Rudel T, Goebel W. Metabolic adaptations of intracellullar bacterial pathogens and their mam-malian host cells during infection (“Pathometabolism”). Microbiol Spectr. 2015 Jun 25;3(3). doi:10.1128/microbiolspec.MBP-0002-2014 Medline
• Elsinghorst EA. Measurement of invasion by gentamicin resis-tance. In: Clark VL, Bavoil PM, editors. Bacterial Pathogenesis Part B: Interaction of Pathogenic Bacteria with Host Cells. San Diego (USA): Academic Press; 1994. p.405–420.doi:10.1016/0076-6879(94)36030-8
• Farage MA, Miller KW, Gerberick GF, Saito FH, Ledger WJ, Witkin SS. Innate immunity in the lower female mucosal tract. J Steroids Horm Sci. 2011;02(02):106. doi:10.4172/2157-7536.1000106Funato K, Beron W, Yang CZ, Mukhopadhyay A, Stahl PD. Recon-stitution of phagosome-lysosome fusion in streptolysin O-per-meabilized cells. J Biol Chem. 1997 Jun 27;272(26):16147–16151. doi:10.1074/jbc.272.26.16147 Medline
• Graham LL. Campylobacter fetus adheres to and enters INT 407 cells. Can J Microbiol. 2002 Nov;48(11):995–1007.doi:10.1139/w02-096 Medline
• Haeger JD, Hambruch N, Dantzer V, Hoelker M, Schellander K, Klisch K, Pfarrer C. Changes in endometrial ezrin and cytokeratin 18 expression during bovine implantation and in caruncular endometrial spheroids in vitro. Placenta. 2015 Aug;36(8):821–831.doi:10.1016/j.placenta.2015.06.001 Medline
• Hall GA, Jones PW. A study of the pathogenesis of experimental Salmonella dublin abortion in cattle. J Comp Pathol. 1977 Jan;87(1): 53–65. doi:10.1016/0021-9975(77)90079-2 Medline
• Hu L, Kopecko DJ.Campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells. Infect Immun. 1999 Aug;67(8):4171–4182. Medline
• Iraola G, Forster SC, Kumar N, Lehours P, Bekal S, García-Peña FJ, Paolicchi F, Morsella C, Hotzel H, Hsueh PR, etal. Dis- tinct Campylobacter fetus lineages adapted as livestock pathogens and human pathobionts in the intestinal microbiota. Nat Commun. 2017 Dec;8(1):1367. doi:10.1038/s41467-017-01449-9 Medline
• Irons PC, Schutte AP, van der Walt ML, Bishop GC. Genital campylobacteriosis in cattle. In: Coetzer JAW, Tustin RC, editors. Infectious diseases of livestock with special reference to southern Africa. Cape Town (South Africa): Oxford University Press; 2004. p.159–1468.
• Kelly DJ. Complexity and versatility in the physiology and meta-bolism of Campylobacter jejuni. In: Nachamkin I, Szymanski CM, editors. Campylobacter. Washington, DC (USA): ASM Press; 2008. p.41–62.Kienesberger S, Sprenger H, Wolfgruber S, Halwachs B, Thallin-ger GG, Perez-Perez GI, Blaser MJ, Zechner EL, Gorkiewicz G.Comparative genome analysis of Campylobacter fetus subspecies revealed horizontally acquired genetic elements important for virulence and niche specificity. PLoS One. 2014 Jan 9;9(1):e85491.doi:10.1371/journal.pone.0085491 Medline
• Konkel ME, Garvis SG, Tipton SL, Anderson DE Jr, Ciep- lak W Jr. Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol Microbiol. 1997 Jun;24(5):953–963.doi:10.1046/j.1365-2958.1997.4031771.x Medline
• Konkel ME, Joens LA. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect Immun. 1989 Oct;57(10):2984–2990.Medline
• Konkel ME, Kim BJ, Rivera-Amill V, Garvis SG. Bacterial secreted proteins are required for the internalization of Campylobacter jejuni into cultured mammalian cells. Mol Microbiol. 1999 May; 32(4):691–701. doi:10.1046/j.1365-2958.1999.01376.x Medline
• Lastovica AJ, Skirrow MB. Clinical significance of Campylobacterand related species other than Campylobacter jejuni and C.coli. In: Nachamkin I, Blaser MJ, editors. Campylobacter. Washington, DC (USA): ASM Press; 2000. p.89–120.
• Liu X, Gao B, Novik V, Galán JE. Quantitative proteomics of intracellular Campylobacter jejuni reveals metabolic reprogram-ming. PLoS Pathog. 2012 Mar 8;8(3):e1002562.doi:10.1371/journal.ppat.1002562 Medline
• Louwen R, Nieuwenhuis EES, van Marrewijk L, Horst-Kreft D, de Ruiter L, Heikema AP, van Wamel WJB, Wagenaar JA, Endtz HP, Samsom J, etal.Campylobacter jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide structures. Infect Immun. 2012 Sep;80(9): 3307–3318. doi:10.1128/IAI.06270-11 Medline
• McSweegan E, Walker RI. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148 Medline.
• Monteville MR, Yoon JE, Konkel ME. Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization. Microbiology. 2003 Jan 01;149(1):153–165.doi:10.1099/mic.0.25820-0 Medline
• Moolhuijzen PM, Lew-Tabor AE, Wlodek BM, Agüero FG, Comerci DJ, Ugalde RA, Sanchez DO, Appels R, Bellgard M.Genomic analysis of Campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets. BMC Microbiol. 2009;9(1):86. doi:10.1186/1471-2180-9-86 Medline
• Mooney A, Byrne C, Clyne M, Johnson-Henry K, Sherman P, Bourke B. Invasion of human epithelial cells by Campylobacter upsaliensis. Cell Microbiol. 2003 Nov;5(11):835–847.doi:10.1046/j.1462-5822.2003.00325.x Medline
• Mularoni A, Mahfoudi A, Beck L, Coosemans V, Bride J, Nicollier M, Adessi GL. Progesterone control of fibronectin secre-tion in guinea pig endometrium. Endocrinology. 1992 Nov;131(5): 2127–2132. doi:10.1210/endo.131.5.1425413 Medline
• Munson L, Chandler SK, Schlafer DH. Cultivation of bovine fetal and adult endometrial epithelial cells. J Tiss Cult Met. 1988.11(3): 129–133. doi:10.1007/BF01404265
• Nachamkin I, Szymanski C, Blaser MJ.Campylobacter. Washington, DC (USA): ASM Press; 2008. 749 p.Oelschlaeger TA, Guerry P, Kopecko DJ. Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. Proc Natl Acad Sci USA. 1993 Jul 15;90(14):6884–6888. doi:10.1073/pnas.90.14.6884 Medline
• Salama SM, Newnham E, Chang N, Taylor DE. Genome map of Campylobacter fetus subsp. fetus ATCC 27374. FEMS Microbiol Lett. 1995 Oct;132(3):239–245.doi:10.1111/j.1574-6968.1995.tb07840.x Medline
• Skarzynski DJ, Miyamoto Y, Okuda K. Production of prostag-landin F(2alpha) by cultured bovine endometrial cells in response to tumor necrosis factor alpha: cell type specificity and intracellular mechanisms. Biol Reprod. 2000 May 01;62(5):1116–1120. doi:10.1095/biolreprod62.5.1116 Medline
• Smith T, Taylor MS. Some morphological and biological characters of the spirilla (Vibrio fetus, n. sp.) associated with disease of the fetal membranes in cattle. J Exp Med. 1919 Oct 1;30(4):299-311.doi:10.1084/jem.30.4.299
• Trüper HG; Judicial Commission of the International Committee on Systematics of Prokaryotes. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int J Syst Evol Microbiol. 2005 Jan 01;55(1):519–520.doi:10.1099/ijs.0.63579-0 Medline
• Truttmann MC, Rhomberg TA, Dehio C. Combined action of the type IV secretion effector proteins BepC and BepF promotes invasome formation of Bartonella henselae on endothelial and epithelial cells. Cell Microbiol. 2011 Feb;13(2):284–299.doi:10.1111/j.1462-5822.2010.01535.x Medline
• Véron M, Chatelain R. Taxonomic study of the genus Campylo-bacter Sebald and Véron and the designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Véron. Int J Syst Bacteriol. 1973 Apr 01;23(2):122–134. doi:10.1099/00207713-23-2-122
• Watson RO, Galán JE.Campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes. PLoS Pathog. 2008;4(1):e14. doi:10.1371/journal.ppat.0040014 Medline
• Yang LY, Pei ZH, Fujimoto S, Blaser MJ. Reattachment of surfacearray proteins to Campylobacter fetus cells. J Bacteriol. 1992 Feb;174(4):1258–1267.doi:10.1128/jb.174.4.1258-1267.1992 Medline

Identyfikatory

DOI

10.33073/pjm-2019-022