Influence of cadmium on protein profile of flax varieties (Linum usitatissimum L.)

• Autorzy: Szalata M. , Wrobel T. , Luwanska A. , Szalata M. , Wielgus K.

Warianty tytułu

PL

Wpływ kadmu na zmianę profili białkowych odmian lnu (Linum usitatissimum L.)

• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the evaluation of the influence of cadmium on protein profile of flax varieties (Linum usitatissimum L.). Linola and Norlin explants were cultured on control medium Dorothy and with addition of 25 and 75 mg/l Cd(NO3)2. Extracts were separated on DEAE-Cellulose (20 mM Tris-HCl buffer, 0.2-1 M NaCl). Protein content was evaluated by measuring the absorbance at wavelengths 280 and 254 nm. Linola was characterized by proteins occurrence in fractions eluted by 0.4 and 0.6 M NaCl at 25 mg/l of Cd(NO3)2, at 75 mg/l and additionally by 0.3 M NaCl. Norlin showed response in the form of proteins appeared at 0.2, 0.4, 0.5 and 0.6 M NaCl gradient at 25 mg/l of cadmium, at higher concentration in fractions eluted by 0.2, 0.5 and 0.6 M NaCl. Electrophoretic analysis showed an increase in the protein bands intensity above 60 kDa and under 52 kDa in extracts from flax cultivated with Cd(NO3)2. Studies showed appearance of new proteins during stress condition.

PL

Celem badań była ocena wpływu kadmu na zmianę profili białkowych odmian lnu (Linum usitatissimum L.). Eksplantaty Linola i Norlin hodowano na podłożu kontrolnym Dorota oraz z dodatkiem 25 i 75 mg/l Cd(NO3)2. Ekstrakty rozdzielano na kolumnie DEAE-Celuloza (20 mM Tris-HCl bufor, gradient 0,2-1 M NaCl). Zawartość białek oceniano przez pomiar absorbancji przy długości fali 280 i 254 nm. Linola charakteryzowała się pojawieniem białek we frakcji wymywanej 0,4 i 0,6 M NaCl przy niższym stężeniu kadmu, natomiast przy 75 mg/l azotanu kadmu dodatkowo we frakcjach wymywanych 0,3 M NaCl. Norlin wykazała obecność białek we frakcjach wymywanych 0,2, 0,4, 0,5 i 0,6 M NaCl w obecności 25 mg/l Cd(NO3)2 w podłożu, przy większym stężenie kadmu pojawiły się białka we frakcjach wymywanych 0,2, 0,5 oraz 0,6 M NaCl. Analiza elektroforetyczna wykazała wzrost intensywności pasm białek w zakresie powyżej 60 kDa i poniżej 52 kDa pod wpływem kadmu w obu odmianach lnu. Przedstawione badania potwierdziły pojawienie się nowych białek w warunkach stresu kadmowego.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2014
• Tom: 60
• Numer: 3
• Opis fizyczny: p.66-76,fig.,ref.

Twórcy

autor

Szalata M.

• Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland

autor

Wrobel T.

• Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland

autor

Luwanska A.

• Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland

autor

Szalata M.

• Department of Biochemistry and Biotechnology, Poznan University of Life Science, Dojazd 11, 60-632 Poznan, Poland

autor

Wielgus K.

• Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland

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Identyfikatory

DOI

10.2478/hepo-2014-0017