Assorted response of mutated variants of vanilla planifolia Andr. towards drought

• Autorzy: Chandran S. , Puthur J. T.
• Języki publikacji: EN

Abstrakty

EN

The mutated variants of Vanilla planifolia Andr. exhibited much variability in their morphology as well as their growth rate. While all the variants and control recorded a decrease in the leaf chlorophyll content and O₂ evolution rate on being subjected to drought stress, in V₃ alone, the decrease in the O₂ evolution rate did not vary much from its unstressed genotypes. The limited distribution of stomata in V₃ (1.65) as compared to other variants and control plants ensured sufficient gas exchange for an efficient photosynthesis, at the same time helps to counter the water loss effectively. The high level of lipid peroxidation in V₄ and V₅ points out towards its inefficiency in utilizing the incident light when exposed to drought, which was otherwise found to be optimal under normal conditions, whereas, the MDA level was lesser in V₃ (only 22% more than watered) as compared to other variants. Proline and carotenoids were found to have a significant role in the detoxification of the free radicals in V₃ as compared to the role of peroxidase, whereas in other variants and control plants, peroxidase was found to have a prominent role in free radical scavenging. The enhanced synthesis of proteins observed in stressed plants of C₁, V₂ and V₃ might help to alleviate the stress-related damage to the system. The enhanced pool of free amino acids in these plants supplements the enhanced rate of protein synthesis observed in them. Total carbohydrates were found to increase in plants subjected to water stress, which contributes towards the osmotic adjustment. The results of this study denote the different level of drought tolerance of mutated variants of Vanilla planifolia Andr.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 5
• Opis fizyczny: p.1023-1029,fig.,ref.

Twórcy

autor

Chandran S.

• Department of Botany, St. Thomas College, Pala, Mahatma Gandhi University, Arunapuram, Kottayam 686 574, Kerala, India

autor

Puthur J. T.

• Department of Botany, St. Thomas College, Pala, Mahatma Gandhi University, Arunapuram, Kottayam 686 574, Kerala, India
• Department of Botany, University of Calicut, CU Campus PO, Tehnipalam, Calicut, Kerala, India

Bibliografia

• Ali M, Jenson CR, Mogensen VO, BahrunA(1999) Drought adaptation of field grown wheat in relation to soil physical condition. Plant Soil 208:149–159. doi:10.1023/A:1004535819197
• An-Lhout F, Zunzunegui FA, Diaz Barradas MC, Tinado R, Clavijio A, Garcia Novo F (2001) Comparison of proline accumulation in two Meditteranean shrubs subjected to natural and experimental water deficit. Plant Soil 230:175–183. doi:10.1023/A:1010387610098
• Anonymous (2002) Vanilla status paper. Spices Board, Govt. of India
• Arnon DI (1949) Copper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15. doi:10.1104/pp.24.1.1
• Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 82:1227–1238
• Asada AK (1984) Chloroplast formation of active oxygen and its scavenging. Methods Enzymol 105:422–428. doi:10.1016/S0076-6879(84)05059-X
• Bates LS, Waldren RP, Teare IK (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205. doi:10.1007/BF00018060
• Bird RP, Draper HH (1984) Comparative studies on different methods of MDA determination. Methods Enzymol 105:299–305. doi: 10.1016/S0076-6879(84)05038-2
• Chandrasekhara RP, Vijnanabhaiah SW (1993) Drought induced lipid peroxidation; defensive mechanism in upland rice (Oryza sativa) seeds during germination. Adv Plant Sci 6:229–230
• Chiang HH, Dandekar AM (1995) Regulation of proline accumulation in Arabidopsis thaliana (L). Heynh; during development and in response to desiccation. Plant Cell Environ 18:1280–1290. doi:10.1111/j.1365-3040.1995.tb00187.x
• Corcuera LT, Hintz M, Pahlich E (1989) Effect of polyethylene glycol on protein extraction and enzyme activation in potato cell cultures. Phytochem 28:569–1591. doi:10.1016/S0031-9422(00) 97801-4
• Dimerevska K, Simova-Stoilova L, Vassileava V, Vaseva I, Grigorova B, Feller U (2008) Drought induced leaf protein alteration in sensitive and tolerant wheat variants. Gen. Appli. Plant Physiol 34(1–2):79–102
• Dubois MK, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Calorimetric method for determination of of sugars and related substance. Anal Chem 28:350–356. doi:10.1021/ac60111a017
• Hare PD, Cress WA (1997) Metabolic implications of stress induced proline accumulation in plants. Plant Growth Regul 21:79–102. doi:10.1023/A:1005703923347
• Hare PD, Cress WA, Van staden J (1998) Directing the roles of osmolyte accumulation during stress. Plant Cell Environ 21:535–553. doi:10.1046/j.1365-3040.1998.00309.x
• Hare PD, Cress WA, Van Staden J (1999) Proline synthesis, degradation; a model system for elucidating stress related signal transduction. J Exp Bot 50:413–434. doi:10.1093/jexbot/50.333.413
• Heath RL, Parker L (1968) Photoperoxidation in isolated chloroplasts. stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198. doi:10.1016/0003-9861(68)90654-1
• Hensdry GAF, Price AH (1993) Stress indicators chlorophyll and carotenoids. In: Hendry GAF, Grime JP (eds) Methods in comparative plant ecology. Chamman and Hall, London, pp 148–152
• Holbrook NN, Putz FE (1996) From epiphyte to tree; difference in leaf structure and leaf water relation associated with transition in growth form in eight species of hemiepiphytes. Plant Cell Environ 19:631–642. doi:10.1111/j.1365-3040.1996.tb00398.x
• Ingram J, Bartel D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47:377–403. doi:10.1146/annurev.arplant.47.1.377
• Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis, the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349. doi:10.1146/annurev.pp.42.060191.001525
• Kuriakose LS, Grace TB, Nazeem PA (2005) Induction of variability in Vanilla through invitro techniques. Proceedings of national symposium on biotechnological interventions for improvement of horticultural crops: issues and strategies. ICAR 8:4–86
• Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficit in higher plants. Plant Cell Environ 25:275–294. doi:10.1046/j.0016-8025.2001.00814.x
• Lowrey OH, Rosenbough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–276
• Maluszynski M, Ahloowalia BS, Sigurbjornsson B (1995) Application of in vivo and in vitro mutation techniques for crop improvement. Euphytic 85:303–315. doi:10.1007/BF00023960
• Matysik J, Alia, Bhalu B, Mohanty P (2002) Molecular mechanism of quenching of reactive oxygen species by proline under stress in plants. Curr Sci 82:525–531
• Morgan JM (1984) Osmoregulation and water stress in higher plants. Ann Rev. Plant Physiol 35:299–319. doi:10.1146/annurev.pp.35. 060184.001503
• Nanjo T, Kobayashi K, Yoshiba Y, Sanada Y, Wada K, Tsukaya H, Kakubari Y, Yamaguchi-Schinozaki K (1999) Biological function of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana. Plant J 18:185–193. doi:10.1046/j.1365-313X.1999.00438.x
• Powles SB (1984) Photoinhibition induced by visible light. Annu Rev Plant Physiol 35:15–44. doi:10.1146/annurev.pp.35.060184.000311
• Puthur JT (2005) Influence of light intensity on growth and productivity of Vanilla planifolia Andr. J Gen Appl Plant Physiol 31(3–4):215–224
• Puthur JT, Krishnakumar V (2006) Studies on the role of metabolites and nutrients on flowering in Vanilla planifolia Andr. J Plant Crops 34(2):90–93
• Puthur JT, Prasanna R (2006) Photosynthetic characteristics and antioxidant mechanism in Vanilla planifolia Andr. exposed to varying intensities of solar radiation. J Plant Crops 34(3):606–611
• Rosen A (1957) A modified ninhydrin calorimetric analysis for amino acids. Arch Biochem Biophys 67:10–15. doi:10.1016/0003-9861 (57)90241-2
• Schneider K, Wells B, Schmelzer E, Salamini F, Bartels D (1993) Desiccation leads to the rapid accumulation of both cytosolic and chloroplastic proteins in the resurrection pant Craterostigma plantagineum Hochst. Planta 189:120–131. doi:10.1007/BF00201352
• Shanggum Z, Shao M, Dyckmani J (1999) Interaction of osmotic adjustment and photosynthesis in winter wheat under soil drought. J Plant Physiol 154:753–758
• Siedow JN (1991) Plant Lipoxygenace structure and function. Annu Rev Plant Physiol Plant Mol Biol 42:145–188. doi:10.1146/annurev.pp.42.060191.001045
• Smirnoff V, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. J Photochem 28:1057–1060. doi: 10.1016/0031-9422(89)80182-7
• Smith BM, Morissey PJ, Guenther JE, Nemson JA, Harrison AJ, Allen F, Melis A (1990) Response of the photosynthetic apparatus in Dunaliella salina to intertidal stress. Plant Physiol 93:1433–1440. doi:10.1104/pp.93.4.1433
• Strauss-Debenedeffi S, Bazzaz FA (1991) Plasticity acclimation to light in tropical moraceae of different successional positions. Oecologia 87:377–387. doi:10.1007/BF00634595
• Verbrugger N, Hua XJ, May M, Van Montagu M (1996) Environmental and developmental signals modulate proline homeostasis; evidence for a negative transcriptional regulation. Proc Natl Acad Sci USA 93:8787–8791. doi:10.1073/pnas.93.16.8787
• Vieira G (1996) Gap dynamics in managed Amazonian forest; structural and ecophysiological aspects. University of Oxford, Oxford, 162p
• Zaltko SZ, Ivan TY (2005) Effect of soil drought on photosynthesis and chlorophyll fluorescence in bean plant. Bulg. Plant Physiol 3–4:3–18
• Zhang J, Kirkham MB (1996) Enzymatic responses of the ascorbate glutathione cycle to drought in sorgham and sunflower plants. J. Plant Sci. 113:139–147. doi:10.1016/0168-9452(95)04295-4
• Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:243–273. doi:10.1146/annurev.arplant. 53.091401.143329

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