Single hibernating males of Myotis alcathoe were recorded in caves and mining adits at four localities in the Beskid Wyspowy Mts, Western Bieszczady Mts, Western Sudety Mts and Roztocze Upland. These are the first records of wintering individuals of the species in Poland and some of the first in Central Europe. Characters are described that may be useful for the identification of adult bats in winter roosts.