Halotolerance (ionic NaCl) and chaotolerance (ionic MgCl2) of the human pathogen Wallemia mellicola isolated, for the first time, from indoor air in Poland

• Autorzy: Chlebicki A. , Spisak W. , Saluga M.
• Języki publikacji: EN

Abstrakty

EN

We isolated the human pathogen Wallemia mellicola from the class Wallemiomycetes for the first time in Poland. The fungus was isolated from indoor dust in Cracow. The strain belonged to the genus Wallemia, as confirmed by molecular methods (i.e., ITS1-5.8S-ITS2 nrDNA sequencing). We compared the halotolerance and chaotolerance of W. mellicola to other those of halotolerant fungi (Talaromyces diversus and Aureobasidium pullulans) isolated from an anchialine cave. The W. mellicola strain tolerated up to 20% NaCl and up to 15% MgCl₂. As the concentration of NaCl in the culture medium increased, the colony diameter of W. mellicola decreased slightly. Dose–response curves for the two reference fungi T. diversus and A. pullulans revealed much lower tolerance of these fungi to increasing concentrations of NaCl. Our results indicated that W. mellicola is an advanced kosmotrope that is distinctly adapted to saline environments.

• Wydawca: -
• Czasopismo: Acta Mycologica
• Rocznik: 2018
• Tom: 53
• Numer: 1
• Opis fizyczny: Article 1104 [6p.], fig.,ref.

Twórcy

autor

Chlebicki A.

• W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland

autor

Spisak W.

• Research and Development Center “Alcor” Ltd, Kepska 12, 45-130 Opole, Poland

autor

Saluga M.

• W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31-512 Krakow, Poland

Bibliografia

• 1. Zalar P, de Hoog GS, Schroers HJ, Frank JM, Gunde-Cimerman N. Taxonomy and phylogeny of the xerophilic genus Wallemia (Wallemiomycetes and Wallemiales, cl. et ord. nov.) Antonie Van Leeuwenhoek. 2005;87:311–328. https://doi.org/10.1007/s10482-004-6783-x
• 2. Nguyen HDT, Jančič S, Meijer M, Tanney JB, Zalar P, Gunde-Cimerman N, et al. Application of the phylogenetic species concept to Wallemia sebi from house dust and indoor air revealed by multi-locus genealogical concordance. PLoS One. 2015;10(3):0120894. https://doi.org/10.1371/journal.pone.0120894
• 3. Jančič S, Nguyen HDT, Frisvad JC, Zalar P, Schroers HJ, Seifer KA, et al. The taxonomic revision of Wallemia sebi species complex. PLoS One. 2015;10(5):e0125933. https://doi.org/10.1371/journal.pone.0125933
• 4. Hoog GS, Guarro J, Figueras MJ. Atlas of clinical fungi [CD-ROM]. 3rd ed. Utrecht: Centraalbureau voor Schimmelcultures.
• 5. Zajc J, Liu Y, Dai W, Yang Z, Hu J, Gostinčar C, et al. Genome and transcriptome sequencing of the halophile fungus Wallemia ichthyophaga: haloadaptations present and absent. BMC Genomics. 2013;14:617. https://doi.org/10.1186/1471-2164-14-617
• 6. Padamsee M, Kumar TK, Riley R, Binder M, Boyd A, Calvo AM, et al. The genome of the xerotolerant mold Wallemia sebi reveals adaptations to osmotic stress and suggests cryptic sexual reproduction. Fungal Genet Biol. 2012;49(3):217–226. https://doi.org/10.1016/j.fgb.2012.01.007
• 7. Wood GM, Mann PJ, Lewis DF, Reid WJ, Moss MO. Studies on a toxic metabolite from the mould Wallemia. Food Addit Contam. 1990;7(1):69–77. https://doi.org/10.1080/02652039009373822
• 8. Frank M, Kingston E, Jeffery JC, Moss MO, Murray M, Simpson TJ, et al. Walleminol and Walleminone, novel caryophyllens from the toxigenic fungus Wallemia sebi. Tetrahedron Lett. 1999;40:133–136. https://doi.org/10.1016/S0040-4039(98)80039-7
• 9. Takahashi I, Maruta R, Ando K, Yoshida M, Iwasaki T, Kanazawa J, et al. UCA1064-B, a new antitumor antibiotic isolated from Wallemia sebi: production, isolation and structural determination. J Antibiot (Tokyo). 1993;46(8):1312–1314. https://doi.org/10.7164/antibiotics.46.1312
• 10. Desroches TC, McMullin DR, Miller JD. Extrolites of Wallemia sebi, a very common fungus in the built environment. Indoor Air. 2014;24(5):533–542. https://doi.org/10.1111/ina.12100
• 11. Guarro J, Gugnani HC, Sood N, Batra R, Mayayo F, Gené J, et al. Subcutaneous phaeohyphomycosis caused by Wallemia sebi in an immunocompetent host. J Clin Microbiol. 2008;46:1129–1131. https://doi.org/10.1128/JCM.01920-07
• 12. Sakamoto T, Urisu A, Yamada M, Matsuda Y, Tanaka K, Torii S. Studies on the osmophilic fungus Wallemia sebi as an allergen evaluated by skin prick test and radioallergosorbent test. Int Arch Allergy Appl Immunol. 1989;90:368–372. https://doi.org/10.1159/000235055
• 13. Zeng QY, Wetermark SO, Rasmunson-Lestander A, Wang XR. Detection and quantification of Wallemia sebi in aerosols by real-time PCR, conventional PCR, and cultivation. Appl Environ Microbiol. 2004;70:7295–7302. https://doi.org/10.1128/AEM.70.12.7295-7302.2004
• 14. Jančič S, Zalar P, Kocev D, Schroers HJ, Džerovski S, Gunde-Cimerman N. Halophily reloaded: new insights into the extremophilic life style of Wallemia with the description of Wallemia hederae sp. nov. Fungal Divers. 2016;76:97–118. https://doi.org/10.1007/s13225-015-0333-x
• 15. Särkinen T, Staats M, Richardson JE, Cowan RS, Bakker FT. How to open the treasure chest? Optimising DNA extraction from herbarium specimens. PLoS One. 2012;7(8):e43808. https://doi.org/10.1371/journal.pone.0043808
• 16. Healey A, Furtado A, Cooper T, Henry RJ. Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species. Plant Methods. 2014;10:21. https://doi.org/10.1186/1746-4811-10-21
• 17. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
• 18. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York, NY: AcademicPress; 1990. p. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
• 19. Zajc J, Džeroski S, Kocev D, Oren A, Sonjak S, Tkavc R, et al. Chaophilic and chaotolerant fungi: a new category of extremophiles? Front Microbiol. 2014;5:708. https://doi.org/10.3389/fmicb.2014.00708
• 20. Hashmi MH, Morgan-Jones G. Conidium ontogeny in hyphomycetes. The meristem arthrospores of Wallemia sebi. Can J Bot. 1973;51:1669–1671. https://doi.org/10.1139/b73-214
• 21. Madelin MF, Dorabjee S. Conidium ontogeny in Wallemia sebi. Transactions of the British Mycological Society. 1974;63:121–130. https://doi.org/10.1016/S0007-1536(74)80143-9
• 22. Moore RT. A note on Wallemia sebi. Antonie Van Leeuwenhoek. 1986;52:183–187. https://doi.org/10.1007/BF00429322

Identyfikatory

DOI

10.5586/am.1104