EN
Koziorowski, M., Stefańczyk-Krzymowska, S., Czarnocki, J. and Krzymowski, T.: Counter.,current transfer and badk transport of 3H-PGF₂ α in the cow's broad ligament vasculature ipsilateral and contralateral to the corpus luteum. Acta, physiol, poll., 1989, 40 (1): 96-103. Eight cowis of similar age (5-7 yeras) were chosen for the experiment. Isolated reproductive tract was supplied with autologous oxygenated and heated (40 ⁰C) blood through the uterine artery and ovaitian artery. 3H-PGF₂α in totial dose of 2 MBq (10⁷ cpm) was injected into each of the uterine lumen of isollaited organ. Blood samples were collected at 5 min intervals during .120 min of experiment using cannulae inserted into the branches of uterine arteries about 1 cm below the horns and from ovarian arteries inserted 0.5 cm below the ovaries. The concentration of 3H-PGF₂α found in blood plasma taken from uterine artery or from ovarian artery on the side wiith active corpus luteum (CL) was significantly lower (p < 0.001) compare with contralateral side to active CL. Radioactive PGF₂α found in.branches of uterine arteries on both lipsiilateral and contralateral side to CL was significantly higher i(p < 0.001) compare to ovarian artery Of the. same side.It is concluded that absorption of 3H-PGF₂α from uterine lumen into venous blood as well as its counter current transfer in area of broad ligament vasculature were reduced on the side of uterine horn with active CL probably as an effect of estrogen : progesteron ratio on vascular constriction in area of Uterine vasculature.