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2010 | 59 | 3 |

Tytuł artykułu

rDNA-based genotyping of clinical isolates of Candida albicans

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study presents an analysis of the restriction pattern of rDNA fragments of 95 C. albicans isolates previously classified on the basis of the presence of the intron in rDNA into genotypes A (62 isolates), B (28), and C (5). Most isolates (61) with genotype A were classified as "subtype a" and one as "subtype d" (Karahan and Akar; 2005). No differences were observed in the restriction patterns of the tested genotype B isolates. Similarly, most genotype C strains (4/5) showed the same restriction pattern. The results indicate low subtyping variations of the analyzed isolates, which is in contrast to published data obtained from a Turkish collection of yeasts.

Wydawca

-

Rocznik

Tom

59

Numer

3

Opis fizyczny

p.213-216,fig.,ref.

Twórcy

autor
  • Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland
  • Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland
  • Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland
autor
  • Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland

Bibliografia

  • Girish Kumar C.P., A. M. Hanafy, M. Katsu, Y. Mikami and T. Menon. 2006. Molecular analysis and susceptibility profiling of Candida albicans isolates from immunocompromised patients in South India. Mycopathologia 161: 153-159.
  • Gurbuz M. and I. Kaleli. 2010. Molecular analysis of Candida albicans isolates from clinical specimens. Mycopathologia 169: 261-267.
  • Jayaguru P. and M. Raghunathan. 2007. Group I intron renders differential susceptibility of Candida albicans to bleomycin. Mol. Biol. Rep. 34: 11-17.
  • Karahan Z.C. and N. Akar. 2005. Subtypes of genotype A Candida albicans isolates determined by restriction endonuclease and sequence analyses. Microbiol. Res. 160: 361-366.
  • Karahan Z.C., H. Guriz, H. Agirbasli, N. Balaban, J.S. Gocmen, D. Aysev and N. Akar. 2004. Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness. Mycoses. 47: 465-469.
  • McCullough M.J., K.V. Clemons and D.A. Stevens. 1999a. Molecular epidemiology of the global and temporal diversity of Candida albicans. Clin. Infect. Dis. 29: 1220-1225.
  • McCullough M.J., K.V. Clemons and D.A. Stevens. 1999b. Molecular and phenotypic characterisation of genotypic Candida albicans subgroups and comparison with Candida dubliniensis and Candida stellatoidea. J. Clin. Microbiol. 37:417-421.
  • Mercure S., S. Montplaisir and G. Lemay. 1993. Correlation between the presence of a self-splicing intron in the 25S rDNA of C. albicans and strains susceptibility to 5-fluorocytosine. Nucleic. Acids. Res. 21: 6020-6027.
  • Miletti-González K.E. and M.J. Leibowitz. 2008. Molecular characterization of two types of rDNA units in a single strain of Candida albicans. J. Eukaryot. Microbiol. 55: 522-529.
  • Millar B.C., J. Xu, R. McMullan, M.J. Walker, S. Hedderwick and J.E. Moore. 2005. Frequency and distribution of group I intron genotypes of Candida albicans colonizing critically ill patients. Br. J. Biomed. Sci. 62: 24-27.
  • Millar B.C., J.E. Moore, J. Xu, M.J. Walker, S. Hedderwick and R. McMullan. 2002. Genotypic subgrouping of clinical isolates of Candida albicans and Candida dubliniensis by 25S intron analysis. Lett. Appl. Microbiol. 35: 102-106.
  • Nawrot U, J. Skala, A. Noczynska, N. Potocka, K. Koczocik and E. Baran. 2004. Distribution of Ca.LSU intron and acid protease production by Candida albicans strains isolated from gastrointestinal tract of diabetes children. Pol. J. Microbiol. 53: 189-191.
  • Nawrot U., J. Skala, K. Włodarczyk, P-A Fonteyne, N. Nolard, J. Nowicka. 2008. Proteolytic activity of clinical Candida albicans isolates in relation to genotype and strain source. Pol. J. Microbiol. 57: 27-33.
  • O'Donnel K., E. Cigelnik, N.S. Weber and J.M. Trappe. 1997 Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis. Mycologia 89: 48-65.
  • Qi Q.G., T. Hu and X.D. Zhou. 2005. Frequency, species and molecular characterization of oral Candida in hosts of different age in China. J. Oral. Pathol. Med. 34: 352-356.
  • Tamura M., K. Watanabe, Y. Mikami, K. Yazawa and K. Nishimura. 2001. Molecular characterization of new clinical isolates of C. albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron. J. Clin. Microbiol. 39: 4309-4315.
  • Zhang Y., Z. Li, D.S. Pilch and M.J. Leibowitz. 2002. Pentamidine inhibits catalytic activity of group I intron Ca.LSU by altering RNA folding. Nucleic. Acids. Res. 30: 2961-2971.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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