Growth and ginsenoside production in Panax quinquefolium hairy roots cultivated in flasks and nutrient sprinkle bioreactor

• Autorzy: Kochan E. , Krolicka A. , Chmiel A.
• Języki publikacji: EN

Abstrakty

EN

Transformed roots of Panax quinquefolium were obtained after inoculation of sterile parts of plantlets from field cultivation with Agrobacterium rhizogenes ATCC 15834. Hairy roots grew in shaken flasks with liquid Gamborg medium supplemented with 30 g saccharose l⁻¹. Effective scale-up of a root culture was achieved in nutrient sprinkle bioreactor. Growth of biomass in shaken flask and in bioreactor increased 10-times and 5-times, respectively. Ginsenoside content exceeds 3 mg/g d.w. in flasks and 6 mg/g d.w. in bioreactor.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 4
• Opis fizyczny: p.1513-1518,fig.,ref.

Twórcy

autor

Kochan E.

• Chair of Biology and Pharmaceutical Biotechnology, Department of Biosynthesis of Drugs, Medical University in Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Krolicka A.

• Department of Biotechnology, Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland

autor

Chmiel A.

• Chair of Biology and Pharmaceutical Biotechnology, Department of Biosynthesis of Drugs, Medical University in Lodz, Muszynskiego 1, 90-151 Lodz, Poland

Bibliografia

• Aoyama T, Hirayama T, Tamamoto S, Oka A (1989) Putative start codon TTG for the regulatory protein VirG of the hairy-rootinducing plasmid pRiA4. Gene 78:173–178
• Atelle AS, Wu JA, Yuan CS (1999) Ginseng pharmacology: multiple constituents and multiple actions. Biochemical Pharmacol 58:1685–1693
• Chmiel A, Pawłowska B, Ledakowicz S (2001) The mist bioreactor for cultures of transformed roots. In: 28th international conference of Slovak Society of Chemical Engineering
• Court EW (2000) Ginseng the genus Panax. Medicinal and aromatic plants: industrial profiles, vol 15. Harwood Academic Publishers, Amsterdam
• Cusido RM, Mallol A, Morales C, Palazon J, Bonfill M, Pinol MT (1998) Ginsenoside production in Panax ginseng hairy roots. role of root morphology. Ginseng in Europe. In: Christian weber H, Zeuske D, Imhof S (eds) Proceedings of the 1st European Ginseng Congress, Marburg 1998, pp 189–198
• Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
• Inomata S, Yokoyama M, Gozu Y, Shimizu T, Ynagi M (1993) Growth pattern and ginsenoside production of Agrobacterium—transformed Panax ginseng roots. Plant Cell Rep 12:681–686
• Jeong G-T, Park D-H, Ryu H-W, Lee W-T, Park K, Kang Ch-H, Hwang B, Woo J-Ch (2002) Optimum conditions for transformed Panax ginseng hairy roots in flask culture. Appl Biochem Biotechnol 98–100:1129–1139
• Kim J, Wyslouzil BE, Weathers PJ (2002) Secondary metabolism of hairy roots cultures in bioreactors. In Vitro Cell Dev Biol-Plant 38:1–10
• Kochan E, Kolodziej B, Gadomska G, Chmiel A (2008) Ginsenoside contents in Panax quinquefolium organs from field cultivation. Z Naturforsch C 63:91–95
• Królicka A, Staniszewska I, Bielawski K, Maliński E, Szafranek J, Łojkowska E (2001) Establishment of hairy root cultures of Ammi majus. Plant Sci 160:259–264
• Królicka A, Szpitter A, Stawujak K, Barański R, Gwizdek-Wiśniewska A, Skrzypczak A, Kamiński M, Łojkowska E (2010) Teratomas of Drosera capensis var. Alba as a source of naphthoquinone: ramentaceone. Plant Cell Tiss Organ Cult 103:285–292
• Lee SH, Jung BH, Kim SY, Lee EH, Chung BCh (2006) The antistress effect of ginseng total saponin and ginsenoside Rg3 and Rb1 evaluated by brain polyamine level under immobilization stress. Pharmacol Res 54:46–49
• Lee J, Jung E, Lee J, Huh S, Kim J, Park M, So J, Ham J, Jung K, Hyun Ch-G, Kim YS, Park D (2007) Panax ginseng induces human type I collagen synthesis through activation of Smad signaling. J Ethnopharmacol 109:29–34
• Lloyd AM, Barnson AR, Rogers SG, Byrne HG, Fraley RT, Hansch RB (1986) Transformation of arabidopsis thaliana with agrobacterium rhizogenes. Science 234:464–466
• Mallol A, Cusido RM, Palazon J, Bonfill M, Morales C, Pinol MT (2001) Ginsenoside production in different phenotypes of Panax ginseng transformed roots. Phytochemistry 57:365–371
• Mathur A, Gangwar A, Mathur AK, Verma P, Uniyal GC, Lal KR (2010) Growth kinetics and ginsenosides production in transformed hairy roots of American ginseng-Panax quinquefolium L. Biotechnol Lett 32:457–461. doi:10.1007/s10529-009-0158-3
• Palazon J, Mallol A, Eibl R, Lettenbauer Ch, Cusido RM, Pinol MT (2003) Growth and ginsenoside production in hairy root cultures of Panax ginseng using a novel bioreactor. Planta Med 69:344–349
• Pirtilla AM, Hirsikorpi M, Kamarainen T, Jaakola L, Hohtola A (2001) DNA isolation methods for medicinal and aromatic plants. Plant Mol Biol Rep 19:273a–273f
• Rai D, Bhatia G, Sen T, Palit G (2003) Anti-stress effect of Ginko biloba and Panax ginseng: a comparative study. J Pharmacol Sci 93:458–464
• Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
• Washida D, Shimomura K, Takido M, Kitanaka S (1998) Ginsenoside in hairy roots of Panax hybrid. Phytochemistry 49:2331–2335
• Washida D, Shimomura K, Nakajima Y, Takido M, Kitanaka S (2004) Auxins affected ginsenoside production and growth hairy roots in Panax hybrid. Biol Pharmaceutical Bull 27(5):657–660
• Woo S–S, Song J-S, Lee J-Y, In DS, Chung H-J, Liu JR, Choi D-W (2004) Selection of high ginsenoside producing ginseng hairy root lines using targeted metabolic analysis. Phytochemistry 65:2751–2761

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