Blastocystis isolates from a dog and their owners presenting with chronic diarrhoea. Dogs as reservoirs of Blastocystis: research in Poland and worldwide

• Autorzy: Kaczmarek A. , Rocka A. , Wesolowska M. , Golab E. , Salamatin R.
• Języki publikacji: EN

Abstrakty

EN

Blastocystis cf. hominis is an unicellular protozoan parasite commonly found in the gastrointestinal tract of humans and animals. Blastocystis is characterized by high morphological and genetic diversity. Studies based on the analysis of Blastocystis spp. small subunit ribosomal RNA genes (SSU rDNA) have identified 26 subtypes (ST) so far, including at least 10 isolated from humans (STs 1–9 and ST12). In 2017, stool samples from a dog and its two owners living in Gdynia, Poland were examined; all three were suffering from chronic diarrhoea. In addition, 30 faecal samples were also examined from 30 dogs kept in one of Warsaw’s hotels for animals. Stool specimens were analyzed using anaerobic cultivation at 37°C with a modified Jones’ medium and molecular methods (PCR). Phylogenetic analysis using Bayesian inference was performed. Vacuolar forms of Blastocystis were identified in the stool samples of the dog and its owners; Blastocystis were not detected in any sample from the dogs living in the animal hotel. Based on the phylogenetic analysis, the obtained isolates were classified as subtype ST3 (for Owner 1) and subtype ST7 (for Owner 2 and the dog). To the best of our knowledge, the present study is the first to evaluate the presence of Blastocystis in canines in Poland, including domestic dogs.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2020
• Tom: 66
• Numer: 4
• Opis fizyczny: p.573-579,fig.,ref.

Twórcy

autor

Kaczmarek A.

• Faculty of Medicine, Collegium Medicum, Cardinal Stefan Wyszynski University in Warsaw, Kazimierza Woycickiego 1/3, 01-938 Warsaw, Poland

autor

Rocka A.

• W. Stefanski Institute of Parasitology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818 Warsaw, Poland

autor

Wesolowska M.

• Department of Biology and Medical Parasitology, Wrocław Medical University, ul. J. Mikulicza-Radeckiego 9, 50-345 Wrocław, Poland

autor

Golab E.

• The National Institute of Public Health – National Institute of Hygiene, ul. Chocimska 24, 00-791 Warsaw, Poland

autor

Salamatin R.

• Faculty of Medicine, Collegium Medicum, Cardinal Stefan Wyszynski University in Warsaw, Kazimierza Woycickiego 1/3, 01-938 Warsaw, Poland
• Department of General Biology and Parasitology, Medical University of Warsaw, ul. Chałubinskiego 5, 02-004 Warsaw, Poland

Identyfikatory

DOI

10.17420/ap6604.300