The effect of cytokinin types and their concentration on in vitro multiplication of Clematis viticella (L.) and Clematis integrifolia ‘Petit Faucon’

• Autorzy: Parzymies M. , Dabski M.

Warianty tytułu

PL

Wpływ rodzaju i stężenia cytokinin na rozkrzewianie in vitro Clematis viticella (L.) i Clematis integrifolia ‘Petit Faucon’

• Języki publikacji: EN

Abstrakty

EN

Determination of the optimal types and concentrations of plant growth regulators as medium constituents is one of the most important factors of successful micropropagation. With the aim of optimization of in vitro multiplication of Clematis viticella and Clematis ‘Petit Faucon’ the effect of following cytokinins on growth and branching was studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ). The obtained results show that KIN at concentration of 10 mg·dm⁻³or 5 mg 2iP·dm⁻³ are the best for proliferation of Clematis viticella shoot tips while nodal parts produce more axillary shoots on the media with 2iP at concentration of 0.62 mg·dm⁻³. Shoot tip explants of Clematis ‘Petit Faucon’ produce the most axillary shoots in the presence of 20 mg 2iP·dm⁻³ or 10 mg KIN·dm⁻³ and nodal ones branch best on the media with addition of 1.25 mg 2iP·dm⁻³.

PL

Określenie rodzaju i stężenia regulatorów wzrostu w pożywce jest jednym z najważniejszych czynników udanego mikrorozmnażania. W celu optymalizacji rozmnażania in vitro Clematis viticella i Clematis integrifolia ‘Petit Faucon’ zbadano wpływ następujących cytokinin: kinetyny (KIN), izopentenyloadeniny (2iP), benzyloadeniny (BA) oraz tidiazuronu (TDZ) na wzrost i rozkrzewianie pędów Clematis viticella i Clematis ‘Petit Faucon’ in vitro. Stwierdzono, że do rozkrzewiania ekplantatów wierzchołkowych Clematis viticella najbardziej odpowiednią cytokininą jest KIN w stężeniu 10 mg·dm⁻³ lub 5 mg 2iP·dm⁻³, natomiast dla eksplantatów węzłowych lepsza okazała się 2iP w stężeniu 0,62 mg·dm⁻³. Ekplantaty wierzchołkowe Clematis ‘Petit Faucon’ najlepiej rozkrzewiają się w obecności 2iP w stężeniu 20 mg·dm⁻³ lub KIN w stężeniu 10 mg·dm⁻³, a węzłowe na pożywce zawierającej 2iP w stężeniu 1,25 mg·dm⁻³.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 1
• Opis fizyczny: p.81-91,ref.

Twórcy

autor

Parzymies M.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, Leszczynskiego 58, 20-068 Lublin, Poland

autor

Dabski M.

Bibliografia

• Bicknell R.A., Braun R.H., Evans A.C., Morgan E.R., 1996. Tissue culture of Ranunculus lyalii Hook. F. New Zealand J. Crop Hort. Sci., 24, 303–306.
• Bliss C.I., 1938. The transformation of percentages for use in the analysis of variance. The Ohio J. Sci., 38, 1, 9–12.
• Dąbski M., Parzymies M., 2006. Wpływ cytokinin na namnażanie powojnika całolistnego (Clematis integrifolia L.) in vitro. Zesz. Probl. Post. Nauk Roln. 510, 119–125.
• Giri C.C., Shyamkumar B., Anjaneyulu C., 2004. Progress in tissue culture, genetic transformation and applications of biotechnology of trees: an overview. Trees 18, 115–135.
• Grey-Wilson C., 2000. Clematis the genus. B. T. Batsford Ltd, London UK.
• Hosoki T., Kobayakawa H., Ohta K., 2003. Micropropagation of chocolate cosmos (Cosmos atrosanguineus) by repeated division of nodes/ axillary shoots and adventitious shoots from microshoots. Acta Hort. 625, 261–265.
• Huang L.C., Huang B.L., Murashige T., 1998. A micropropagation protocol for Cinnamomum camphora. In Vitro Cell Dev. Biol. Plant 34, 141–146.
• Ibrahim R., Debergh P.C., 2000. Improvement of adventitious bud formation and plantlet regeneration from in vitro leaflet explants of roses (Rosa hybrida L.). Acta Hort. 520, 271–275.
• Isnard S., Speck T., Rowe N.P., 2003. Mechanical architecture and development of Clematis: implications for canalised evolution of growth forms. New Phytologist 158, 543–559.
• Kim M-S., Klopfenstein N.B., Cregg B.M., 1998. In vitro and ex vitro rooting of micropropagated shoots using three green ash (Fraxinus pennsylvanica) clones. New Forests 16, 43–57.
• Kreen S., Svensson M., Rumpunen K., 2002. Rooting of clematis microshoots and stem cuttings in different substrates. Scientia Hort. 96, 351–357.
• Kyung-Ku Shim, Yoo-Mi Ha, 1997. New gold leaf cultivar of Forsythia koreana (‘Suwon Gold’) and its mass propagation in vitro. Acta Hort. 107, 64–69.
• Lu M-C., 2005. Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb, through high-frequency shoot tip culture. Scientia Hort. 107, 64–69.
• Mereti M., Grigoradiou K., Nanos G.D., 2002. Micropropagation of the strawberry tree, Arbutus unedo L. Scientia Hort. 93, 143–148.
• Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.
• Nesterowicz S., Kulpa D., Moder K., Kurek J., 2006. Micropropagation of an old specimen of common lilac (Syringa vulgaris L.) from the dendrological garden at Przelewice. Acta Sci. Pol. Hortorum Cultus 5(1), 27–35.
• Nobre J., Santos C., Romano A., 2000. Micropropagation of the Mediterranean species Viburnum tinus. Plant Cell Tiss. Org. Cult. 60, 75–78.
• Palacios N., Christou P., Leech M.J., 2002. Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultural stem explants. Plant Cell Rep. 20, 808–813.
• Raja Naika H., Krishna V., 2008. Plant regeneration from callus culture of Clematis gouriana Roxb. – A rare medicinal plant. Turkish J. Biology 32(2), 99–103.
• Sahoo Y., Chand P.K., 1998. Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation. Plant Cell Rep. 18, 301–307
• Sansberro P., Rey H., Mroginski L., 2003. In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae). Trees 17, 542–546.
• Vengadesan G., Ganapathi A., Amutha S., Selvaraj N., 2002. In vitro propagation of Acacia species – a review. Plant Science 163, 663–671.
• Zhang Q.X., Hu H.K., Wang A.X., Fang Y.M., 2011. Somatic embryogenesis and plant regeneration of clematis ‘Multi-Blue’. Propagation of Ornamental Plants 11(1), 21–27.