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2014 | 17 | 2 |
Tytuł artykułu

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
17
Numer
2
Opis fizyczny
p.367-369,ref.
Twórcy
autor
  • Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland
autor
  • Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland
autor
  • Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland
autor
  • National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
  • Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroclaw, Poland
autor
  • National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
Bibliografia
  • Becker A, Lutz-Wohlgroth L, Brugnera E, Lu ZH, Zimmermann DR, Grimm F, Grosse Beilage E, Kaps S, Spiess B, Pospischil A, Vaughan L (2007) Intensively kept pigs pre-disposed to chlamydial associated conjunctivitis. J Vet Med A Physiol Pathol Clin Med 54: 307-313.
  • Camenisch U, Lu ZH, Vaughan L, Corboz L, Zimmermann DR, Wittenbrink MM, Pospischil A, Sydler T (2004) Diagnostic investigation into the role of Chlamydiae in cases of increased rates of return to oestrus in pigs. Vet Rec 155: 593-596.
  • Englund S, Segerstad CH, Arnlund F, Westergren E, Jacobson M (2012) The occurrence of Chlamydia spp. in pigs with and without clinical disease. BMC Vet Res 8: 1-6.
  • Everett KD, Hornung LJ, Andersen AA (1999) Rapid detection of the Chlamydiaceae and other families in the order Chlamydiales: three PCR tests. J Clin Microbiol 37: 575-580.
  • Kauffold J, Melzer F, Henning K, Schulze K, Leiding C, Sachse K (2006) Prevalence of chlamydiae in boars and semen used for artificial insemination. Theriogenology 65: 1750-1758.
  • Rypuła K, Niemczuk K, Kumala A, Płoneczka-Janeczko K, Pejsak Z (2012) Epidemiologic aspects of Chlamydia spp. infections in swine. Med Weter 9: 517-520.
  • Schautteet K, Vanrompay D (2011) Chlamydiaceae infections in pig. Vet Res 42: 1-10.
  • Vanrompay D, Geens T, Desplanques A, Hoang TQT, De Vos L, Van Loock M, Huyck E, Miry C, Cox E (2004) Immunoblotting, ELISA and culture evidence for Chlamydiaceae in sows on 258 Belgian farms. Vet Microbiol 99: 59-66.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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