Genetic diversity and population structure assessment of Chinese Senna obtusifolia L. by molecular markers and morphological traits of seed

• Autorzy: Mao R. , Xia P. , Liu J. , Li X. , Han R. , Liu F. , Zhao H. , Liang Z.
• Języki publikacji: EN

Abstrakty

EN

Senna obtusifolia L. is an important medicinal plant in Asia. This study was the first report on the genetic diversity and population structure of S. obtusifolia which were collected from 47 geographic populations widespread in China. Inter-Simple Sequence Repeat (ISSR) and Start Codon Target Polymorphism (SCoT) combined with seeds morphological traits were used to investigate the relationship of 47 populations. 11 ISSR primers yielded 98 polymorphic bands with 81.67% polymorphism. 24 SCoT primers yielded 267 polymorphic bands with 89.59% polymorphism. The number of allele (Na), the number of effective allele (Ne), Nei’s diversity index (H), and Shannon’s information index (I) reflected a high level of genetic diversity of S. obtusifolia species. The greatest genetic distance (G D) existed between Southwest and Northwest (0.4022ISSR/0.5019SCoT), while the Eastern and Northern showed the least genetic distance (0.1751ISSR/0.2186SCoT). The genetic differentiation (Gst) was 0.4875ISSR/0.4434SCoT, and the gene flow (Nm) was 0.5256ISSR/0.6275SCoT, which indicated that gene exchange among four regions was limited. 47 samples were divided into four clusters mainly according to their geographic distribution through clustering and structure analysis. The analysis on the combined data of ISSR and SCoT showed more reliable and superior results than single analysis of ISSR and SCoT. This study explored the effectiveness of ISSR and SCoT markers to evaluate the genetic diversity and population structure of S. obtusifolia and provided useful information for S. obtusifolia germplasm research and breeding program.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 01
• Opis fizyczny: Article 12 [14p.], fig.,ref.

Twórcy

autor

Mao R.

• State and Local Joint Research Center of TCM Fingerprint and NP Library, College of Life Sciences, Northwest Aand F University, Yangling 712100, Shaanxi, China

autor

Xia P.

• School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China

autor

Liu J.

• State and Local Joint Research Center of TCM Fingerprint and NP Library, College of Life Sciences, Northwest Aand F University, Yangling 712100, Shaanxi, China

autor

Li X.

• State and Local Joint Research Center of TCM Fingerprint and NP Library, College of Life Sciences, Northwest Aand F University, Yangling 712100, Shaanxi, China

autor

Han R.

• State and Local Joint Research Center of TCM Fingerprint and NP Library, College of Life Sciences, Northwest Aand F University, Yangling 712100, Shaanxi, China

autor

Liu F.

• Shaanxi TASLY Plants Pharmaceutical Co. Ltd, Shangluo 726000, Shaanxi, China

autor

Zhao H.

• Shaanxi TASLY Plants Pharmaceutical Co. Ltd, Shangluo 726000, Shaanxi, China

autor

Liang Z.

• School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China

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Identyfikatory

DOI

10.1007/s11738-017-2586-3