Dimethyl fumarate prevents spatial working memory impairment and does not affect brain IL-6 expression in streptozotocininducted rat model of Alzheimer's disease

• Autorzy: Majkutewicz I. , Kurowska E. , Myslinska D. , Grembecka B. , Grzybowska M. , Podlacha M. , Rucinski J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Animal model of sporadic form of Alzheimer Disease (AD) evoked by intracerebroventricular (icv) injection of betacytotoxic drug, streptozotocin (STZ), reflects memory impairments, brain hypometabolism, cholinergic deficit, activation of microglia and neurodegeneration found in AD patients. Brain inflammation is important factor contributing to exacerbation of AD symptoms, but some studies show neuroprotective properties of pro-inflammatory cytokine IL-6. The aim of this study was to determine if dimethyl fumarate (DMF), which has anti-oxidative and immunosuppressive properties, can alleviate spatial working memory impairments in STZ-icv induced rat model of AD and change the expression of IL-6 in the brains. METHODS: Four experimental groups were separated: STZ DMF (n=8) – STZ-icv infused and fed with 0.4% DMF fodder for three weeks until spatial memory test of Morris, STZ CTR (n=8) – STZicv infused and fed with standard fodder, and VEH DMF and VEH CTR groups (n=10) – vehicle-icv infused and fed with 0.4% DMF or standard fodder, respectively. Morris water maze testing was performed for three days, with four trials per day with unchanged platform location, and rats were then sacrificed and brains subjected to immunofluorescent IL-6 labeling. RESULTS: The latency to reach the platform in each trial was significantly longer in the STZ CTR rats than in the remaining groups. STZ CTR was the only group which did not decrease the latency and the distance swum to platform in the consecutive trials. STZicv infused rats  (STZ CTR and STZ DMF groups) had also lower number of the IL-6 expressing cells in the hippocampus and the hypothalamus than control VEH CTR rats. CONCLUSION: Oral medication with DMF prevents spatial working memory impairment evoked by STZ-icv infusion, but has no influence on the central expression of IL-6. The study was financed by the National Science Centre Poland on the basis of decision DEC-2013/09/D/NZ4/01658.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S38

Twórcy

autor

Majkutewicz I.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Kurowska E.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Myslinska D.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Grembecka B.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Grzybowska M.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Podlacha M.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland

autor

Rucinski J.

• Department of Animal and Human Physiology, University of Gdansk, Gdansk, Poland