Micropropagation of Orchis catasetum-a rare and endangered orchid

• Autorzy: Baker A. , Kaviani B. , Nematzadeh G. , Negahdar N.

Warianty tytułu

PL

Mikrorozmnażanie Orchis catasetum-rzadkiej i zagrożonej orchidei

• Języki publikacji: EN

Abstrakty

EN

The application of modern biotechnology for mass propagation of rare and endangered species needs to develop a proper in vitro protocol. Here, a protocol was developed for high frequency in vitro multiplication of an endangered orchid, Orchis catasetum. Protocorms, as explants were cultured on Murashige and Skoog (MS) medium fortified with different concentrations of N6 -benzyladenine (BA), Į-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) either individually or in combination. A combination of 0.5 mg·L BA and 0.5 mg·L NAA was found to be suitable for maximum protocorm-like bodies (PLBs) regeneration (20.40 × plantlet⁻¹). The largest number of root (7.16 × plantlet⁻¹) and leaf (10.10 × plantlet⁻¹), also the highest plant height (114.20 mm × plantlet⁻¹) and root length (193.40 mm × plantlet⁻¹) were obtained on MS medium supplemented with 0.5 mg·L BA a long with 0.5 mg · L NAA. Plantlets with well-developed leaves and roots were transplanted to pots filled with perlite, wood pieces, ionolite and mineral cartridge shell (1:1:1:1), also perlite individually and transferred to the greenhouse. Upon ex vitro transfer, 100% of plants survived.

PL

Zastosowanie nowoczesnej biotechnologii do masowego rozmnażania rzadkich i zagrożonych gatunków wymaga opracowania właściwej procedury in vitro. W niniejszych badaniach opracowano metodę wysokiej częstotliwości rozmnażania in vitro zagrożonej orchidei Orchis catasetum. Protokormy, jako eksplanty, były hodowane na pożywce Murashige and Skoog (MS) wzmocnionej różnymi stężeniami N6 -benzyloadeniny (BA), kwasu 1-naftylooctowego (NAA) oraz kwasu indolilomasłowego (IBA), pojedynczo lub w kombinacji. Stwierdzono, ze kombinacja 0,5 mg · L BA i 0,5 mg · L NAA była odpowiednia do maksymalnej regeneracji PLB (20,40 × sadzonka⁻¹). Największą liczbę korzeni (7,16 × sadzonka⁻¹) i liści (10,10 × sadzonka⁻¹), a także największą wysokość roślin (114,20 mm × roślina⁻¹) i długość korzeni (193,40 mm × sadzonka⁻¹) uzyskano na pożywce MS uzupełnionej za pomocą 0,5 mg · L BA razem z 0,5 mg · L NAA. Sadzonki o dobrze rozwiniętych liściach i korzeniach były przeniesione do doniczek wypełnionych perlitem, trocinami, jonolitem i kompleksem minerałów (1:1:1:1), a także samym perlitem, po czym zostały przeniesione do cieplarni. Po przeniesieniu ex vitro 100% roślin przeżyło.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 2
• Opis fizyczny: p.197-205,fig.,ref.

Twórcy

autor

Baker A.

• Islamic Azad University, Rasht, Iran

autor

Kaviani B.

• Rasht Branch, Department of Horticultural Science, Islamic Azad University, Rasht, Iran

autor

Nematzadeh G.

• Agricultural Sciences and Natural Resources University, Sari, Iran

autor

Negahdar N.

• Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran

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