Identification and phylogenic analysis of Leishmania species among patients and reservoir hosts based on N-acetylglucosamine-1-phosphate transferase gene in Central Iran

• Autorzy: Farrokhi-Karibozorg M. , Hajjaran H. , Ghayour-Najafabadi Z. , Hejazi S.H. , Ataei-Pirkooh A. , Mohebali M.
• Języki publikacji: EN

Abstrakty

EN

Leishmaniosis, a vector-born disease that infects humans and other vertebrates, is the result of infection with Leishmania species belong to the family Trypanosomatidae. The present study was performed to determine the status of cutaneous leishmaniosis in Isfahan province. Samples were taken from the margin of skin ulcers of patients with suspected CL referred to the medical health centers in Isfahan province. Also, ear and snout samples were taken from the rodents. In total, 85 parasitologically positive samples were subjected to the PCR-RFLP method based on the nagt gene for identification of Leishmania species, also 11 samples were subjected to sequencing and phylogenetic analysis. For all positive samples, a 1450–1460 bp band of the nagt gene was amplified in PCR method. The digestion pattern of ACC1 enzyme in 79 of patients indicated L. major and in one sample was similar to L. tropica. Four rodent reservoirs distingue as L. major and one sample as L. turanica. Phylogenetic analysis confirmed the species identification and three haplotypes were reported. The results of the current study showed that L. major is the predominant species of Leishmania parasites in Isfahan province and the main reservoir of CL is Rhombomys opimus. Also, the nagt gene is a useful and practical marker for determining different species of Leishmania parasites as well as their phylogenetic analysis.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2022
• Tom: 68
• Numer: 3
• Opis fizyczny: p.461-471,fig.,ref.

Twórcy

autor

Farrokhi-Karibozorg M.

• Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

autor

Hajjaran H.

• Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

autor

Ghayour-Najafabadi Z.

• Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

autor

Hejazi S.H.

• Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
• Department of Parasitology and Mycology, Center for Research in Skin Diseases and Leishmaniasis, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

autor

Ataei-Pirkooh A.

• Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

autor

Mohebali M.

• Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Identyfikatory

DOI

10.17420/ap6803.452